Inotropic and cardioprotective effects of Daucus carota Linn. on isoproterenol-induced myocardial infarction

Authors

  • P. Muralidharan Department of Pharmacology and Toxicology, C. L. Baid Metha College of Pharmacy, Jyothi Nagar, Thoraipakkam, Chennai 97, Tamil Nadu
  • G. Balamurugan Department of Pharmacology and Toxicology, C. L. Baid Metha College of Pharmacy, Jyothi Nagar, Thoraipakkam, Chennai 97, Tamil Nadu
  • Pavan Kumar Department of Toxicology, Hi-tech City, Madhapur, Andhra Pradesh 500081

DOI:

https://doi.org/10.3329/bjp.v3i2.849

Keywords:

Cardioprotective, Daucus carota, Inotropic, Isoproterenol

Abstract

Daucus carota Linn. tubers were extracted with water and analyzed for its inotropic and cardioprotective effects by measuring various biochemical parameters at the test doses of 250 and 500 mg/kg. Isoproterenol (5.25 mg/kg and 8.5 mg/kg) was administered subcutaneously on 29th and 30th day respectively in order to induce myocardial infarction. Cardiac tonicity was estimated by evaluating  Na+K+ATPase, Mg2+ATPase and Ca2+ATPase levels in heart. The levels of Na+K+ATPase and Mg2+ATPase were decreased and that of Ca2+ATPase was increased in extract-treated group significantly (p<0.001).  Cardioprotection was assessed by estimating serum aspartate transaminase, alanine transaminase, lipid peroxidase, and lactate dehydrogenase levels and cardiac total protein and lipid peroxidase, and lactate dehydrogenase. The levels altered by isoproterenol were restored significantly by the administration of the extract. The result of the study implies that D. carota is a potential source to protect heart from myocardial infarction and to maintain its tonicity.

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Author Biographies

P. Muralidharan, Department of Pharmacology and Toxicology, C. L. Baid Metha College of Pharmacy, Jyothi Nagar, Thoraipakkam, Chennai 97, Tamil Nadu

Professor and Head

G. Balamurugan, Department of Pharmacology and Toxicology, C. L. Baid Metha College of Pharmacy, Jyothi Nagar, Thoraipakkam, Chennai 97, Tamil Nadu

Research Scholar

References

Akera T, Brody TM. The role of Na+ K+ ATPase in the ionotropic actions of digitalis. Pharmacol Rev. 1997; 29: 187-220.

Alam H. Varley Practical clinical biochemistry. 6th ed. Gove luck, 2002, pp 522-23.

Allard JP, Royall D, Kurian R, Muggli R, Jeejeebhoy K. Effects of carotene supplementation on lipid peroxidation in humans. Am J Clin Nutr. 1994; 59: 588-90.

Beller GA, Smiy TW, Abelmann WH, Haber E. Digitalis intoxication, a prospective clinical study with serum level correlations. New Engl J Med. 1971; 284: 989-97.

Bishayee A, Sarkar A, Chaterjee M. Hepatoprotective activity of carrot against carbon tetrachloride intoxication in mouse liver. J Ethanopharmacol. 1995; 47: 69-74.

Bonting SL. Sodium-potassium activated adenosine triphosphate and cation transport. In: Membrane and ion transport. Bittar EE (ed). London, Wiley Interscience, 1970.

Burton GN, Ingold KV. ?-carotene, an unusual type of lipid antioxidant. Science 1984; 24: 569-76.

Chaterjee A, Prakesh S. The treatise of Indian medicinal plants. Vol 3, India, Publication and Information Directorate (CSIR), 1995, pp 38-40.

Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. India, CSIR, 1992, p 50.

Ecobichon DJ. The basis of toxicology testing. New York, CRC Press, 1997, pp 43-86.

Goto A, Yamada K, Yagi N. Physiological and pharmacology of endogenous digitalis like factors. Pharmacol Rev. 1992; 44: 377- 99.

Hjerten S, Pan H. Purification and characterization of two forms of low affinity Ca2+ATPase from erythrocyte membranes. Biochem Biophys Acta. 1983; 728: 281-88.

Kitada Y, Narimatsu A, Suzuki R. Does the positive ionotropic action of novel cardiotonic agent involve mechanism other than cyclic AMP. J Pharmacol. 1987; 243: 639-45.

Knekt P, Reunanen A, Jarvinen R, Seppanen R, Heliovaara M, Aroma A. Antioxidant vitamin intake and coronary mortality in a longitudinal population study. Am J Epidemiol. 1994; 139: 1180-89.

Kritikar KR, Basu BD, Indian medicinal plants. Vol 2. 2nd ed. Bombay, International Book Distributors, 1981, p 1229.

Lowry OH, Rosebrough NJ, Farr AL. Protein measurements with the Folin phenol reagent. J Biol Chem. 1951: 193: 265-68.

Mohur AF, Cooke Y. Simple method of measuring serum level of glutamate oxaloacetic acid and glutamate pyruvate transaminase in routine laboratories. J Clin Path. 1979; 193: 265-68.

Nieland A. Lactic acid dehydrogenase of heart muscle. In: Methods in enzymology. Vol 1. New York, Academic Press, 1955, p 394.

Ohinisi T, Suzuki T, Suzuki Y, Ozawa K. Comparative study of plasma membrane Mg2+ATPase in normal regenerating and malignant cells. Biochem Biophys Acta. 1982; 684: 67-74.

Okhawa H, Onishi N. Assay of lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351.

Prabhu S, Mallika Jainu K, ShyamalaDevi CS. Cardio protective effect of magnifera on isoproterenol-induced myocardial infarction in rats. Indian J Exper Biol. 2006; 44: 109-25.

Prochezhian E, Ansari SH. Analgesic and anti-inflammatory activity of volatile oil from Daucus carota Linn. Indian J Nat Prod. 2000; 16: 24-26.

Reitman S, Frankel S. A colorimetric method of measuring serum level of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase. Am J Clin Path. 1957; 28: 56.

Rona G, Chappel CJ, Balazs T, Gaudry R. An infarct like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. Arch Path. 1959; 76: 443-55.

Senthil Kumar H, Anandan R, Santhosh Kumar M. Cardioprotective effect of Picorrhiza kurrooa against isoproterenol-induced myocardial stress in rats. Fitotherapia 2001; 72: 402.

Suchalata S, ShyamalaDevi CS. Protective effect of Terminalia chebula against experimental myocardial injury induced by isoproterenol. Indian J Exp Biol. 2004; 42: 174.

Additional Files

Published

2008-06-08

How to Cite

Muralidharan, P., G. Balamurugan, and P. Kumar. “Inotropic and Cardioprotective Effects of Daucus Carota Linn. On Isoproterenol-Induced Myocardial Infarction”. Bangladesh Journal of Pharmacology, vol. 3, no. 2, June 2008, pp. 74-79, doi:10.3329/bjp.v3i2.849.

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Section

Research Articles