In vitro free radical scavenging activity of Ixora coccinea L
Antioxidant activity of the methanol extract of Ixora coccinea L. was determined by DPPH free radical scavenging assay, reducing power and total antioxidant capacity using phosphomolybdenum method. Preliminary phytochemical screening revealed that the extract of the flower of I. coccinea possesses flavonoids, steroids and tannin materials. The extract showed significant activities in all antioxidant assays compared to the standard antioxidant in a dose-dependent manner and remarkable activities to scavenge reactive oxygen species (ROS) may be attributed to the high amount of hydrophilic phenolics. In DPPH radical scavenging assay the IC50 value of the extract was found to be 100.5 μg/mL while ascorbic acid had the IC50 value 58.9 μg/mL. Moreover, I. coccinea extract showed strong reducing power and total antioxidant capacity.
Alam MA, Nyeem MAB, Awal MA, Mostofa M, Alam MS, Subhan N, Rahman MM. Antioxidant and hepatoprotective action of the crude methanolic extract of the flowering top of Rosa damascena. OPEM. 2008; 8: 164-70.
Alam MA, Ghani A, Subhan N, Rahman MM, Haque MS, Majumder MM, Mazumder MEH, Akter RA, Nahar L, Sarker SD. Antioxidant and membrane stabilizing properties of the flowering tops of Anthocephalus cadamba. Nat Pro Commun. 2008; 3: 65-67.
Annapurna J, Amarnath PVS, Amar Kumar D, Ramakrishna SV, Raghavan KV. Antimicrobial activity of Ixora coccinea leaves. Fitoterapia 2003; 74: 29193.
Boynes JW. Role of oxidative stress in the development of complication in diabetes. Diabetes 1991; 40: 40511.
Braca A, Sortino C, Politi M, Morelli I, Mendez J. Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol. 2002; 79: 379-81.
Branen AL. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc. 1975; 52: 59-63.
Collier A, Wilson R, Bradley H, Thomson JA, Small M. Free radical activity in type 2 diabetes. Diabetes 1990; 7: 2730.
Duh PD, Tu YY, Yen GC. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum moifolium Ramat). LebensmWiss Technol. 1999; 32: 269-77.
Fernandez MA, De las Heras B, Garcia MD, Saenz MT, Villar A. New insights into the mechanism of action of the anti-inflammatory Triterpene lupeole. J Pharm Pharmacol. 2001; 53: 1533-39.
Ghani A. Medicinal plants of Bangladesh with chemical constituents and uses. Dhaka, The Asiatic Society of Bangladesh, 2003, p 267.
Gordon MH. The mechanism of the antioxidant action in vitro. In: Food antioxidants. Hudson BJF (ed.). London, Elsevier, 1990, pp 118.
Gyamfi MA, Aniya Y. Antioxidant properties of thonningianin A, isolated from the African medicinal herb, Thonningia sanguine. Biochem Pharmacol. 2002; 63: 1725-37.
Hasan MS, Ahmed MI, Mondal S, Uddin SJ, Masud MM, Sadhu SK, Ishibashi M. Antioxidant, antinociceptive activity and general toxicity study of Dendrophthoe falcata and isolation of quercitrin as the major component. OPEM. 2006; 6: 355-60.
Ismaili H, Sosa S, Brkic D, Fkih-Tetouani S, IIidrrissi A, Touati D, Aquino RP, Tubaro A. Topical anti-inflammatory activity of extracts and compounds from Thymus broussonettii. J Pharm Pharmacol. 2002; 54: 1137-40.
Ito N, Fukushima S, Hasegawa A, Shibata M, Ogiso T. Carcinogenicity of butylated anisole in F344 rats. J Natl Cancer Inst. 1983; 70: 343-47.
Jorgensen LV, Madsen HL, Thomsen MK, Dragsted LO, Skibsted LH. Regulation of phenolic antioxidants from phenoxyl radicals: An ESR and electrochemical study of antioxidant hierarchy. Free Radical Res. 1999; 30: 207-20.
Kayano S, Kikuzaki H, Fukutsuka N, Mitani T, Nakatani N. Antioxidant activity of prune (Prunus domestica L.) constituents and a new synergist. J Agric Food Chem. 2002; 50: 3708-12.
Latha PG, Panikkar KR. Chemoprotective effect of Ixora coccinea L. flowers on cisplatin induced toxicity in mice. Phytother Res. 2001; 15: 364-66.
Latha PG, Panikkar KR, Modulatory effects of Ixora coccinea flower on cyclophosphamide-induced toxicity in mice. Phytother Res. 1999, 13: 517-20.
Latha PG, Panikkar KR. Cytotoxic and antitumor principles from Ixora coccinea flowers. Cancer Lett. 1998; 130: 197-202.
Lim SN, Cheung PCK, Ooi VEC, Ang PO. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J Agric Food Chem. 2002; 50: 3862-66.
Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jap J Nutr. 1986; 44: 307-15.
Patocka J. Biologically active pentacyclic triterpenes and their current medicine signification. J Appl Biomed. 2003; 1: 7-12.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999; 269: 33741.
Ragasa CY, Tiu F, Rideout JA. New cycloartenol esters from Ixora coccinea. Nat Prod Res. 2004; 18: 319-23.
Ratnasooriya WD, Deraniyagala SA, Bathige SD, Goonasekara CL, Jayakody JR. Antinociceptive action of aqueous extract of the leaves of Ixora coccinea. Acta Biol Hung. 2005; 56: 21-34.
Ratnasooriya WD, Deraniyagala SA, Galhena G, Liyanage SSP, Bathige SDNK, Jayakody JRAC. Anti-inflammatory activity of the aqueous leaf extract of Ixora coccinea. Pharmaceutical Biol. 2005; 43: 147-52.
Repetto MG, Llesuy SF. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz J Med Biol Res. 2002, 35: 523-34.
Rice-Evans C, Sampson J, Bramley PM, Holloway DE. Why do we expect carotenoids to be antioxidants in vivo. Free Radical Res. 1997; 26: 381-98.
Sabu MC, Kuttan R. Antidiabetic activity of medicinal plants and its relationship with their antioxidant property. J Ethnopharmacol. 2002; 81: 15560.
Trease GE, Evans WC. Pharmacognosy, 12th ed. London, Baillieere Tindal, 1983.
Viturro C, Molina A. Schmeda-Hischmann G. Free radical scavengers from Mutisia friesiana (Asteraceae) and Sanicula graveolens (Apiaceae). Phytother Res. 1999; 13: 422-24.
Copyright (c) 2008 Moni Rani Saha, Md. Ashraful Alam, Raushanara Akter, Rumana Jahangir
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).