Cardioprotective activity of ethanolic extract of Callistemon lanceolatus leaves on doxorubicin-induced cardiomyopathy in rats
Keywords:Antioxidant, Blood pressure, Cardiac toxicity, Doxorubicin, ECG
The cardioprotective effect of ethanol extract of Callistemon lanceolatus leaves (100 and 200 mg/kg) against doxorubicin-induced cardiomyopathy was studied. Rats were pretreated with the extract for 7 days and simultaneous treatment with doxorubicin along with the extracts for the next 14 days. After 24 hours of last dose of doxorubicin, ECG, invasive blood pressure, serum markers (creatine kinase-MB, lactate dehydrogenase, serum transaminases), tissue antioxidant markers (catalase, superoxide dismutase) and extent of lipid peroxidation (malondialdehyde) were studied. The elevated ST segment, decreased blood pressure, increased level of serum enzymes and decreased level of tissue antioxidant markers were observed in doxorubicin treatment (p<0.01). While 200 mg/kg extract significantly reduced the elevated levels of the serum enzymes and restores the ECG and blood pressure to normal, also significantly increased the tissue antioxidant levels, while decreased the malondialdehyde level (p<0.01) when compared with the control. The histopathological study confirmed the cardioprotection.
Aebi H. Catalase. In: Methods of enzymatic analysis. Bergmeyer HU (ed). Verlag, Chemic Academic Press Inc, 1974, pp 673-85.
Ali N, Syed WAS, Ahmad B. Calcium channel blocking activity of fruits of Callistemon citrinus. J Chem Soc Pakistan. 2011; 33: 245-48.
Anonymous. The wealth of India raw material. New Delhi, Council of Scientific & Industrial Research, 1992, pp 63-65.
Billingham ME, Bristow MR, Glatstein E, Spina A, Tacconi MT, Veroni E. Adriamycin cardiotoxicity: Endomyocardial evidence of enhancement by irradiation. Am J Surg Pathol. 1977; 1: 17-23.
Bristo MR, Sageman WS, Scott RH. Acute and chronic cardiovascular effects of doxorubicin in dog. J Cardiovasc Pharmacol. 1980; 2: 487-515.
Chopra S, Pillai KK, Husain SZ, Girl DK. Propolis Protects against doxorubicin-induced myocardiopathy in rats. Exp Mol Pathol. 1995; 62: 190-98.
Chowdhary AK, Saha NK. Inhibition of urd bean leaf crinkle virus by different plant extracts. Indian Phytopathol. 1985; 38: 566-68.
Danesi R, Del Tacca M, Soldani G. Measurement of the ST segment as the most reliable electrocardiogram parameter for the assessment of adriamycin-induced cardiotoxicity in the rat. J Pharmacol Methods. 1986; 6: 251-59.
Dewar HA, Rowell NR, Smith AJ. Serum glutamic oxalacetic transaminase in acute myocardial infarction. Br Med J. 1958; 8: 1121-25.
Ferrari R. The role of mitochondria in ischemic heart disease. J Cardiovasc Pharmacol. 1996; 28: S1-S10.
Garba IH, Ubom GA. Total serum lactate dehydrogenase activity in acute Plasmodium falciparum malaria infection. Singapore Med J. 2005; 46: 632-34.
Geetha A, Devi CS. Effect of doxorubicin on heart mitochondrial enzymes in rats: A protective role for alphatocopherol. Indian J Exp Biol. 1992; 30: 615-18.
Gupta A, Gupta R. A survey of plants for anticholinesterase activity. Phytochemistry 1997; 46: 827-31.
Hanasaki Y, Ogawa S, Fukui S. The correlation between active oxygen scavenging and antioxidative effects of flavonoids. Free Radical Biol Med. 1994; 16: 845-50.
Herman EH, Zhang J, Chadwick DP, Ferrans V. Comparison of the protective effects of amifostine and dexrazoxane against the toxicity of doxorubicin in spontaneously hypertensive rats. Cancer Chemother Pharmacol. 2000; 45: 329-34.
Hoekman K, Van der WJF, Vermorken JB. Clinical and preclinical modulation of chemotherapy-induced toxicity in patients with cancer. Drugs 1999; 57: 133-56.
Jain AK, Dubey SK, Sikarwar MS and Jain SK. Hepatoprotective activity of methanolic extract of leaves of Callistemon lanceolatus. Internat J Plant Sci. 2007; 2: 185-86.
Jain D. Cardiotoxicity of doxorubicin and other anthracycline derivatives. J Nuclear Cardiolog. 2000; 7: 53-62.
Jeong W, Su HS, Kim N, Yang YT, Su SY, Lee C, Hwang BY, Lee D. Bioactive triterpenoids from Callistemon lanceolatus. Arch Pharm Res. 2009; 32: 845-49.
Kalender O, Eylem T, Nurcan D. Protective effect of carnosine on adriamycin-induced oxidative heart damage in rats. Anadolu Kardiyol Derg. 2010; 11: 310.
Khandelwal KR. Practical pharmacognosy, techniques and experiments. 12th ed. Pune, Nirali Prakashan, 2004, pp 152-56.
Kim JH, Byun JC, Bandi AKR, Hyun CG, Lee NH. Compounds with elastase inhibition and free radical scavenging activities from Callistemon lanceolatus. J Med Plants Res. 2009; 3: 914-20.
Korshunov SS, Skulachev VP, Starkov AA. High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria. FEBS Lett. 1997; 416: 15-18.
Kumar S, Kumar V, Prakash O. Antidiabetic, hypolipidemic and antioxidant activities of Callistemon lanceolatus leaves extract. J Herbs Spices Med Plants. 2011; 17: 144-53.
Marklund S. Pyrogallol autooxidation. In: Greenwald RA. Handbook of methods for oxygen radical research. Boca raton, CRC Press, 1985, pp 243-47.
Minotti G, Menna P, Salvatorelli E, Cairo G, Gianni L. Anthracyclines: Molecular advances and pharmacologic developments in antitumor activity and cardiotoxicity. Pharmacol Res. 2004; 56: 185-229.
Monti EE, Prosperi R, Supino Bottiroli G. Free radical- dependant DNA lesions are involved in the delayed cardiotoxicity-induced by adriamycin in the rat. Anticancer Res. 1995; 15: 193-97.
Morin D, Barthelemy S, Zini R, Labidalle S, Tillement JP. Curcumin induces the mitochondrial permeability transition pore mediated by membrane protein thiol oxidation. FEBS Lett. 2001; 495: 131-36.
Okhawa H, Ohishi N, Yaki K. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-58.
Olson HM, Young DM. Electrolyte and morphologic alterations of myocardium in adriamycin treated rabbits. Am J Pathol. 1974; 77: 439-54.
Rossi F, Filippelli W, Russo S, Filippelli A, Berrino L. Cardiotoxicity of doxorubicin: Effects of drugs inhibiting the release of vasoactive substances. Pharmacol Toxicol. 1994; 75: 99-107.
Saad SY, Najjar TA, AL-Rikabi AC. The preventive role of deferoxamine against acute doxorubicin-induced cardiac, renal and hepatic toxicity in rats. Pharmacol Res. 2001; 43: 211-18.
Sharma RK, Kotoky R, Bhattacharyya PR. Volatile oil from the leaves of Callistemon lanceolatus D. C. grown in north-eastern India. Flavour Fragr J. 2006; 21: 239-40.
Singal PK, Iliskovic N. Doxorubicin-induced cardiomyopathy. N Engl J Med. 1998; 339: 900-5.
Siveski-Iliskovic N, Kaul N, Singal PK. Probucol promotes endogeneous antioxidants and provides protection against adriamycin-induced cardiacmyopathy in rats. Circulation 1994; 89: 2829-35.
Steinhertz LJ, Steinhertz PG, Tan CTC, Heller G, Murphy L. Cardiac toxicity 4 to 20 years after completing anthracycline therapy. J Am Med Assoc. 1991; 266: 1672-77.
Sudhakar M, Rao CV, Rao AL, Raju DB. Antinociceptive and anti-inflammatory effects of the standardized oil of Indian Callistemon lanceolatus leaves in experimental animals. Acta
Pharmaceutica Turcica. 2004; 46: 131-39.
Van Acker SABE, Kramer K, Voest EE, Grimbergen JA, Zhang J, Van der Vijg WJF, Bast A. Doxorubicininduced cardiotoxicity monitored by ECG in freely moving mice. Cancer Chemother Pharmacol. 1996; 38: 95-101.
Wakade AS, Sha AS, Kulkarni MP, Juvekar AR. Protective effect of Piper longum L on oxidative stress induced injury and cellular abnormality in adriamycin-induced cardiotoxicity in rats. Indian J Exp Biol. 2008; 46: 528-33.
Weinberg LE, Singal PK. Refractory heart failure and age-related differences in adriamycin-induced myocardial changes in rats. Can J Physiol Pharmacol. 1987; 65: 1957-65.
You JS, Huang HF, Chang YL, Lee YS. Sheng-Mai-San reduces adriamycininduced cardiomyopathy in Rats. Am J Chinese Med. 2006; 34: 295-305.
Young RC, Myers CE. Adriamycin: The role of lipid peroxidation in cardiac toxicity and tumor response. Science 1977; 19: 165-67.
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