Dietary shochu kasu alleviates fatty liver induced by orotic acid

Authors

  • Yohanes Buang Department of Chemistry, Faculty of Science and Engineering, Nusa Cendana University-Indonesia
  • Pius D. Ola Department of Chemistry, Faculty of Science and Engineering, Nusa Cendana University, Kupang
  • Teruyoshi Yanagita Laboratory of Applied Biochemistry, Department of Applied Biological Science, Saga University

DOI:

https://doi.org/10.3329/bjp.v5i1.5921

Keywords:

Fatty liver, Orotic acid, Triacylglycerol, Shochu kasu

Abstract

The effects of dietary shochu kasu (5%) on orotic acid-induced fatty liver were studied in rats. Liver triglyceride content of orotic acid-fed rats increased by 5-fold in comparison to basal group.  Shochu kasu reduced triglyceride content by two-third.  The activities of fatty acid synthase and phosphatidate phosphohydrolase significantly decreased that was approximately by 35% and 20%, respectively. The serum lipid levels however were nearly unchanged. The alleviation of fatty liver in rats with shochu kasu, therefore, was associated with the inhibition of fatty acid synthase and phosphatidate phosphohydrolase activities.

Downloads

Download data is not yet available.
Abstract
741
Download
684 Read
147

References

Barlett GR. Phosphorous assay in column chromatography. J Biol Chem. 1959; 234: 466-68.

Buang Y, Wang YM, Cha JY, Nagao K, Yanagita T. Dietary phosphatidylcholine alleviates fatty liver induced by orotic acid. Nutrition 2005; 21: 867-73.

Buang Y, Cha JY, Nagao K, Wang YM, Inoue N, Yanagita T. Alleviation of fatty liver by ?-linolenic acid. J Nutr Sci Vitaminol. 2004; 50: 272-76.

Cha JY, Mameda Y, Yamamoto Y, Oogami K, Yanagita T. Association between hepatic triglycerol accumulation induced by administering orotic acid and enhanced phophatidate phosphohydrolase activity in rats. Biosci Biotechnol Biochem. 1998; 62: 508-13.

Cotrim HP, Parana R, Braga E, Lyra L. Nonalcoholic steatohepatitis and hepatocellular carcinoma: Natural history? Am J Gastroenterol. 2000; 95: 3018-19.

Dixon JB, Bhathal PS, OBrien PE. Nonalcoholic fatty liver disease: Predictors of nonalcoholic steatohepatitis and liver fibrosis in the severely obese. Gastroenterology 2001; 121: 91-100.

Fletcher MJ. A colorimetric method for estimating serum triglycerides. Clin Chim Acta 1968; 22: 393-97.

Folch J, Lees M, Sloane-stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957; 226: 497-509.

Harrison SA, Diehl AM. Fat and the liver: A molecular overview. Semin Gastrointest Dis. 2002; 13: 3-16.

Ikeda I, Cha JY, Yanagita T, Nakatani N, Oogami K, Imaizumi K, Yazawa K. Effect of dietary ?-linolenic, eicosapentaenoic, and docosahexaenoic acids on hepatic lipogenesis and ?-oxidation in rats. Biosci Biotechnol Biochem. 1998; 62: 675-80.

Lehninger LA, Nelson DL, Cox MM. Principles of biochemistry. 2nd ed. USA, Worth Publishers, 1993.

Lowry OH, Rosebrough NJ, Farr AL, Randal RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265-75.

Pottenger LA, Getz GS. Serum lipoprotein accumulation in the livers orotic acid-fed rats. J lipid Res. 1971; 12: 450-59.

Sherlock S, Dooley J. Diseases of the liver and biliary system. Oxford, Blackwell Science, 1997.

Starkel P, Sempaoux C, Leclercq I, Herin M, Debby C, Desager JP, Horsmans Y. Oxidative stress, KLF6 and transforming growth factor-? upregulation differentiate non-alcoholic steatohepatitis progressing to fibrosis from uncomplicated steatosis in rats. J Hepatol. 2003; 39: 538-46.

Tsushin NK. The beverage and food statistics monthly. Tokyo, Nikkan Keizai Tsushin, 2005, p 133.

Walton PA, Possmayer F. Mg2-dependent phosphatidate phosphohydrolaseof rat lung: Development of an assay employing a defined chemical substrate which reflects the phosphohydrolase activity measured using membrane-bound substrate. Anal Biochem. 1985; 151: 479-86.

Yamasaki T, Aki T, Shinozaki M, Taguchi M, Kawamoto S, Ono K. Utilization of shochu distillery wastewater for production of polyunsaturated fatty acids and xanthophylls using thraustochytrid. J Biosc Bioeng. 2006; 102: 323-27.

Moon YS, Latasa MJ, Griffin MJ, Sul HS. Suppression of fatty acid synthase promoter by polyunsaturated fatty acids. J Lipid Res. 2002; 43: 691-98.

Yokoyama S, Hiramatsu J, Hayakawa K. Production of ?-aminobutyric acid from alcohol distillery less by lactobacillus brevis IFO-12005. J Biosci Bioeng. 2002; 93: 95-97.

Yokoyama S, Tarumi S. Production and some properties of low-salt seasoning from shochu distillery waste. Seibutsu-Kogaku 2001; 79: 211-17.

Published

2010-09-16

How to Cite

Buang, Y., P. D. Ola, and T. Yanagita. “Dietary Shochu Kasu Alleviates Fatty Liver Induced by Orotic Acid”. Bangladesh Journal of Pharmacology, vol. 5, no. 1, Sept. 2010, pp. 57-61, doi:10.3329/bjp.v5i1.5921.

Issue

Section

Research Articles