Antimicrobial peptides from the hemolymph of the prawn Macrobrachium rosenbergii
Keywords:Antimicrobial activity, Antimicrobial peptide, Prawn
The study was carried out to find the antimicrobial activity of hemolymph of Macrobrachium rosenbergii and to evaluate the antimicrobial compounds. The highest inhibition against Staphylococcus aureus, Lactobacillus vulgaris and Klebsiella pneumonia (12 mm) and antifungal activity was observed only against Fusarium moniliforme (11 mm). Antimicrobial peptide was characterized in molecular size ranging from 22 to 91KDa with antimicrobial activity against various infectious pathogens. Hemolymph plays a vital role in the disease prevention in crustaceans and there is no report on antimicrobial activities of the prawn M. rosenbergii.
Bartlett TC, Cuthbertson BJ, Shepard E, Chapman R, Gross P, Warr G. Crustins, homologues of an 11.5-kDa antibacterial peptide, from two species of penaeid shrimp, Litopenaeus vannamei and Litopenaeus setiferus. Mar Biotechnol. 2002; 4: 278-93.
Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol. 1966; 45: 493-96.
Chiou T, Jen-Leih Wu, Thomas T, Chen L, Jenn-Kan Lu. Molecular cloning and characterization of cDNA of penaeidine: Antimicrobial peptide from tiger shrimp (Penaeus monodon). Marine Biotechnol. 2007; 204: 1013-20.
Chisholm JRS, Smith VJ. Antibacterial activity in hemocytes of the shore crab, Carcinus maenas (L). J Mar Biol Assoc UK. 1992; 72: 529-42.
Cuthbertson BJ, Büllesbach EE, Gross PS. Discovery of synthetic penaeidin activity against antibiotic-resistant fungi. Chem Biol Drug Des. 2006; 68: 12027.
Cuthbertson BJ, Shepard EF, Chapman RW, Gross PS. Diversity of the penaeidin antimicrobial peptides in two shrimp species. Immunogenetics 2002; 54: 44245.
Destoumieux D, Bulet P, Loew D, Dorsselaer AV, Rodriguez J, Bachere E. Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus vannamei (Decapoda). J Biol Chem. 1997; 272: 28398-406.
Destoumieux D, Bulet P, Strub JM, Dorsselaer AV, Bachere E. Recombinant expression and range of activity of penaeidins, antimicrobial peptides from penaeid shrimp. Eur J Biochem. 1999; 266: 335-46.
Destoumieux D, Saulnier D, Garnier J, Jouffrey C, Bulet P, Bachere E. Crustacean immunity: Antifungal peptides are generated from the C terminus of shrimp hemocyanin in response to microbial challenge. J Biol Chem. 1999; 276: 47070-77.
Engstrom Y. Insect immune gene regulation. In: Molecular mechanisms of immune response in insects. Brey PT, Hultmark D (eds). London, Chapman and Hall Publishers, 1999, pp 211-44.
Ferrandon D, Jung AC, Criqui MC, Lemaitre B, Uttenweiler-Joseph S, Michaut L, Reichhart J, Hoffmann JA. A drosomycin- GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. EMBOJ. 1998; 17: 1217-27.
Haug T, Kjuul AK, Stensvag K, Sandsdalen E, Styrvold OB. Antibacterial activity in four marine crustacean decapods. Fish Shellfish Immunol. 2002; 12: 37185.
Hoffmann JA, Reichhart JM. Drosophila immunity. Trends Cell Biol. 1997; 7: 309316.
Iwanaga, S. The molecular basis of innate immunity in the horseshoe crab. Curr Opin Immunol. 2002; 14: 8795.
Kawabata SI, Nagayama R, Hirata M, Shigenaga T, Agarwala KL, Saito T, Cho J, Nakajima H, Takagi T, Iwanaga S. Tachycitin, a small granular component in horseshoe crab hemocytes is an antimicrobial protein with chitin-binding activity. J Biochem. 1996; 120: 125360.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T. Nature 1970; 227; 680-85.
Lowry OH, Rosenbrough JN, Farr AL, Randall RJ. Protein measurement with folin-phenol reagent. J Biol Chem. 1951; 193: 26575. PMid:14907713
Mori K, Stewart JE. The hemolymph bactericidin of the american lobster (Homarus americanus) adsorption and activation. J Fish Res Board Canada. 1978; 5: 150407.
Munoz M, Vandenbulcke F, Garnier J, Gueguen Y, Bulet P, Saulnier D, Bachère E. Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic Vibrio. Cell Mol Life Sci. 2004; 61: 961-72.
Pan JY, Zhang YL, Peng XX. Dodecamer is required for agglutination of Litopenaeus vannamei hemocyanin with bacterial cells and red blood Cells. Mar Biotechnol. 2008; 301: 39-54.
Rameshkumar G, Ravichandran S, Kaliyavarathan G, Ajithkumar TT. Antimicrobial peptide from the crab, Thalamita crenata (Latreille, 1829). World J Fish Marine Sci. 2009; 1: 74-79.
Ravichandran S, Wahidulla S, DSouza L, Rameshkumar G. Antimicrobial lipids from the lemolymph of brachyuran crabs. Appl Biochem Biotechnol. 2010; 162: 1039-51.
Schnapp D, Kemp GD, Smith VJ. Purification and characterization of a proline- rich antibacterial peptide, with sequence similarity to bactenecin-7, from the hemocytes of the shore crab, Carcinus maenas. Eurobian J Biochem. 1996; 240: 532-39.
Smith VJ, Soderhall K. ?-1,3-Glucan activation of crustacean hemocytes in vitro and in vivo. Biol Bull. 1983; 164: 299-314.
Soderhall K, Cerenius L. Role of the prophenoloxidase-activating system in invertebrate immunity. Curr Opin Immunol. 1998; 10: 23-28.
Stewart JE, Zwicker BM. Natural and induced bactericidal activities in the hemolymph of the lobster, Homarus americanus: Products of hemocyte-plasma interaction. Can J Microbiol. 1972; 18: 1499-509.
Supungul P, Klinbunga S, Pichyangkura R, Jitrapakdee S, Hirono I, Aoki T, Tassanakajon A. Identification of immune-related genes in hemocytes of black tiger shrimp (Penaeus monodon). Mar Biotechnol. 2002; 35: 4487-94.
White KN, Ratcliffe NA, Rossa M. The antibacterial activity of haemocyte clumps in the gills of the shore crab, Carcinus maenas. J Mar Biol Assoc UK. 1985; 65: 857-70.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).