Effect of hexane extract of spinach in the removal of arsenic from rat

  • Badar Uddin Umar Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka 1000
Keywords: Arsenic, Hexane extract of spinach, Malondialdehye


Extensive search is going on for a cheap, easily available and effective remedy of chronic arsenic poisoning. The present study was designed to find the effects of hexane extract of spinach in the removal of arsenic from arsenic treated rat. Rats were fed arsenic trioxide through Ryles tube for one month then they were fed on hexane extract (1-4%) of spinach for another one month. Hexane extract of spinach decreased accumulated arsenic from rat liver, spleen, kidney, intestine, lungs and skin significantly. Besides, it reduced the oxidative stress caused by arsenic which was evident by decreased levels of malondialdehye (MDA) in the above tissues. Hexane extract decreases both arsenic level and MDA level in rat tissues in dose dependent manner, which is more effective at lower doses.


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Ahmad SA, Faruquee MH, Sayed MHSU, Khan MH, Jalil MA, Ahmed R, Hadi SA. Chronic arsenicosis: Management by vitamin A, E, C regimen. JPSOM. 1998; 17: 19-26.

Ahmad S, Kitchin KT, Cullen WR. Arsenic species that causes release of iron from ferritin and generation of activated oxygen. Arch Biochem Biophys. 2000; 382: 195-02.

Dey RK. Management protocol for arsenicosis cases. Arsenicosis case-detection, management and surveillance. Report of a regional consultation in New Delhi, India, 5-9 Nov. 2002.

Farber LJ. Mechanism of cell injury by activated oxygen species. Environ Health Perspect. 1994; 102: 17-24.

Guha Mazumder DN, Ghoshal UC, Saha J, Santra A, De, BK, Chatterjee A, Dutta S, Angle CR, Centeno JA. Randomized placebo-controlled trial of 2,3-dimercaptosuccinic acid in therapy of chronic arsenicosis due to drinking arsenic-contaminated subsoil water. J Toxicol Clin Toxicol. 1998; 36: 683-90.

Guha Majumder DN. Arsenic and liver disease. J Ind Med Assoc. 2001; 99: 311, 314-5, 318-20.

Guha Mazumder DN, De BK, Santra A, Ghosh N, Das S, Lahiri S, Das T. Randomized placebo-controlled trial of 2,3-dimercapto-1-propanesulfonate (DMPS) in therapy of chronic arsenicosis due to drinking arsenic-contaminated water. J Toxicol Clin Toxicol. 2001; 39: 665-74.

Hagen TM, Ingersoll RT, Lykkesfeldt J, Liu J, Wehr CM, Vinarsky V, Batholomew JC, Ames BN. (R)-?-Lipoic acid supplemented old rats have improved mitochondrial function, decreased oxidative damage and increased metabolic rate. FASEB J. 1999; 13: 411-18.

Kalia K, Flora SJ. Strategies for safe and effective therapeutic measures for chronic arsenic and lead poisoning. J Occup Health. 2005; 47: 1-21.

Khalequzzaman M, Faruque FS, Mitra AK. Assessment of arsenic contamination of groundwater and health problems in Bangladesh. Int J Environ Res Public Health. 2005; 2: 204-13.

Kokilavani V, Devi MA, Sivarajan K, Panneerselvam C. Combined efficacies of DL ? lipoic acid and meso 2,3 dimercaptosuccinic acid against arsenic induced toxicity in antioxidant systems of rats. Toxicol Lett. 2005; 160: 1-7.

Levander OA. Metabolic inter relationship between arsenic and selenium. Environ Health Perspect. 1977; 19: 159-64.

Lowry OH, Rosenbrough NJ, Farr AL and Randall RJ. Protein measurement with the Folin phenol reagent. The J of Biological Chemistry. 1951; 265-75.

Marafante E, Bertolero F, Edel J, Pietra R, Sabbioni E. Intracellular interaction and biotransformation of arsenic in rats and rabbits. Sci Total Environ. 1982; 24: 27-39.

Masella RT, Benedetto RD, Vari R, Filesi C, Giovannini C. Novel mechanism of natural antioxidant compounds in biological systems: Involvement of glutathione and glutathione related enzymes. J Nutr Biochem. 2005; 16: 577-86.

Misbahuddin M, Islam AZMM, Khandker S, Mahmud IA, Islam N, Anjumanara. Efficacy of spirulina extract plus zinc in patients of chronic arsenic poisoning: A randomized placebo-controlled study. Clinical Toxicol. 2006; 44: 135-41.

Milton AH, Hasan Z, Shahidullah SM, Sharmin S, Jakariya MD, Rahman M, Dear K, Smith W. Association between nutritional status and arsenicosis due to chronic arsenic exposure in Bangladesh. Int J Environ Health Res. 2004; 14: 99-108.

Mitra SR, Guha Mazumder DN, Basu A, Block G, Haque R, Samanta S, Ghosh N, Smith MMH, Ehrenstein OS, Smith AH. Nutritional factors and susceptibility to arsenic-caused skin lesions in West Bengal, India. Environ Health Perspect. 2004; 112: 1104-09.

Nasir M. Effects of exogenous selenium on arsenic accumulation and glutathione level in different tissues of rat. M Phil thesis, Dept of Pharmacology, BSMMU. 2002.

Pi J, Yamauchi H, Kumagai Y, Sun G, Yoshida T, Aikawa H, Rich CH, Shimojo N. Evidence for induction of oxidative stress caused by chronic exposure of Chinese residents to arsenic contained in drinking water. Environ Health Perspect. 2002; 110: 331-36.

Piamphongsant T. Management of arsenicosis. Arsenicosis case-detection, management and surveillance. Report of a regional consultation in New Delhi, India, 5-9 Nov. 2002.

Rabbani GH, Saha SK, Akhtar M, Marni F, Mitra AK, Ahmed S, Alauddin M, Bhattacharjee M, Sultana S, Chowdhury AKA. Antioxidants in detoxification of arsenic-induced oxidative injury in rabbits: Preliminary results. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2003; 38: 273-87.

Rahman M, Chowdhury U, Mukherjee S, Mondal B, Paul K, Lodh D. Chronic arsenic toxicity in Bangladesh and West Bengal, India: A review and commentary. J Toxicol Clin Toxicol. 2001; 39: 683-700.

Ramanathan K, Anusuyadevi M, Shila S, Panneerselvam C. Ascorbic acid and alpha-tocopherol as potent modulators of apoptosis on arsenic induced toxicity in rats. Toxicol Lett. 2005; 156: 297-306.

Ramos O, Carrizales L, Yanez L, Mejia J, Batres L, Ortiz D, Barriga DF. Arsenic increased lipid peroxidation in rat tissues by a mechanism independent of glutathione levels. Environ Health Perspect. 1995; 103: 85-88.

Saha SK, Sikder MS, Zakaria ASM, Ahmed K, Khan MMR, Rahman H, Ray PK, Raihan ASMA, Rahman MT, Hasan M. Chronic arsenic toxicity and liver damage Report of two cases with review of literature. Bangladesh Medical J. 1999; 145.

Saha SK. Effects of hexane extracts of spirulina in the removal of arsenic from isolated liver tissues of rat. Mymensingh Med J. 2005; 14: 191-95.

Schiller CM, Fowler BA, Woods JS. Effects of arsenic on pyruvate dehydrogenase activation. Environ Health Perspect. 1977; 19: 205-07.

Spallholz JE, Porte F La, Alauddin M, Chakravorty B, Marshall JR, Westfall PH, Ross LF, Feldmann J, Gailer J, George G, Ahmed S, Zuberi MI, Shea CR, Robertson JD, Umbaugh SE, Tchounwou PB, Pickering I. Selenium against arsenicosis in Bangladesh and West-Bengal (India). A proposed, placebo-controlled, randomized, double-blind phase-III clinical trial to assess the efficacy of selenium dietary supplements in countering signs of arsenic toxicity in the context of Bangladesh arsenic crisis. 2006; 52.

Tabassum NE. Effect of ?-lipoic acid on the removal of arsenic from arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol. 2006; 1: 27-32.

Uddin MK. Effects of zinc and iron supplementation on arsenic accumulation in different tissues of rat. M Phil thesis, Department of Pharmacology, BSMMU, 2002.

WHO. Arsenic. Environmental Health Criteria 18. Geneva, World Health Organization, 1981.

Xu DP, Wells WW. ? lipoic acid dependent regeneration of ascorbic acid from dehydroascorbic acid in rat liver mitochondria. J Bioenerg Biomembr. 1996; 28: 77-85.

Yoshida T, Yamauchi H, Sun GF. Chronic health effects in people exposed to arsenic via the drinking water: dose-response relationship in review. Toxicol Appl Phrmacol. 2004; 198: 243-52.

How to Cite
Umar, B. “Effect of Hexane Extract of Spinach in the Removal of Arsenic from Rat”. Bangladesh Journal of Pharmacology, Vol. 2, no. 1, 1, pp. 27-34, doi:10.3329/bjp.v2i1.497.
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