Effect of folic acid and tetrahydrofolate on tissue arsenic level in rat


  • Md. Faizur Rahman Department of Pharmacology, Faculty of Basic Sciences, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka 1000
  • Mir Misbahuddin Department of Pharmacology, Faculty of Basic Sciences, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka 1000




Arsenic, Folic acid, Tetrahydrofolate


Quest is still going on for a cheap, effective, and easily available remedy for chronic arsenic toxicity. This study was designed to investigate the effects of folic acid and tetrahydrofolate in lowering the arsenic burden in tissues. Rats received arsenic at 700 µg/day by gavage for 28 days except the control group. Arsenic accumulation was significantly lowered (p<0.05), in liver, kidney, heart, lung and skin in both the folic acid and tetrahydrofolate-treated groups compared to arsenic only treated group. The oxidative stress induced by arsenic treatment was reduced as evident by the reduction in rise of malondialdehyde level in both groups. But folic acid was found to be more efficacious compared to tetrahydrofolate. 


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Balaghi M, Wagner C. Folate deficiency inhibits pancreatic amylase secretion in rats. Am J Clin Nutr. 1995; 61: 90-96.

Baldwin DR, Marshall WJ. Heavy metal poisoning and its laboratory investigation. Ann Clin Biochem. 1999; 36: 267-300.

Blundell G, Jones BG, Rose FA, Tudball N. Homocysteine mediated endothelial cell toxicity and its amelioration. Atherosclerosis 1996; 122: 163-72.

Chowdhury NJA, Misbahuddin M, Rahman MS. Corn extracts lower tissue arsenic level in rat. Bangladesh Med Res Counc Bull. 2009; 35: 21-25.

Clifford AJ, Heid MK, Muller HG, Bills ND. Tissue distribution and prediction of total body folate of rats. J Nutr. 1990; 120: 1633-39.

Gamble MV, Liu X, Ahsan H, Pilsner JR, Ilievski V, Slavkovich V, Parvez F, Chen Y, Levy D, Factor-Litvak P, Graziano JH. Folate and arsenic metabolism: A double-blind, placebo-controlled folic acid-supplementation trial in Bangladesh. Am J Clin Nutr. 2006; 84: 1093-101.

Gamble MV, Liu X, Ahsan H, Pilsner JR, Ilievski V, Slavkovich V, Parvez F, Chen Y, Levy D, Factor-Litvak P, Graziano JH. Folate, homocysteine, and arsenic metabolism in arsenic-exposed individuals in Bangladesh. Environ Health Perspect. 2005; 113: 1683-88.

Gomez-Caminero A, Howe P, Hughes M, Kenyon E, Lewis DR, Moore M. Properties and analytical procedures: Arsenic and arsenic compound. Environmental health criteria 224. International programme on chemical safety. Geneva, World Health Organization, 2001, pp 9-27.

Huang RF, Hsu YC, Lin HL, Yang FL. Folate depletion and elevated plasma homocysteine promote oxidative stress in rat livers. J Nutr. 2001; 131: 33-38.

Jacob RA, Wu MM, Henning SM, Swendseid ME. Homocysteine increases as folate decreases in plasma of healthy men during short-term dietary folate and methyl group restriction. J Nutr. 1994; 124: 1072-80.

Kamaluddin M, Misbahuddin M. Zinc supplement on tissue arsenic concentration in rats. Bang Med Res Coun Bull. 2006; 32: 87-91.

Lindgren A, Vahter M, Dencker L. Autoradiographic studies on the distribution of arsenic in mice and hamsters administered 74As-arsenite or -arsenate. Acta Pharmacol Toxicol. 1982; 51: 253-65.

Majumdar KK, Guha Mazumder DN, Ghose N, Ghose A, Lahiri S. Systemic manifestations in chronic arsenic toxicity in absence of skin lesions in West Bengal. Indian J Med Res. 2009; 129: 75-82.

Meharg AA, Rahman MM. Arsenic contamination of Bangladesh paddy field soils: Implications for rice contribution to arsenic consumption. Environ Sci Technol. 2003; 37: 229-34.

Misbahuddin M. Consumption of arsenic through cooked rice. Lancet. 2003; 361: 435-36.

Misbahuddin M, Islam AZ, Khandker S, Ifthaker-Al-Mahmud, Islam N, Anjumanara. Efficacy of spirulina extract plus zinc in patients of chronic arsenic poisoning: A randomized placebo-controlled study. Clin Toxicol (Phila). 2006; 44: 135-41.

Mitra SR, Mazumdar DN, Basu A, Block G, Haque R, Samanta S, Ghosh N, Smith MM, von Ehrenstein OS, Smith AH. Nutritional factors and susceptibility to arsenic-caused skin lesions in West Bengal, India. Environ Health Perspect. 2004; 112: 1104-09.

Nandi D, Patra RC, Swarup D. Effect of cysteine, methionine, ascorbic acid and thiamine on arsenic-induced oxidative stress and biochemical alterations in rats. Toxicology 2005; 211: 26-35.

Ramos O, Carrizales L, Yanez L, Mejia J, Batres L, Ortiz D, Diaz-Barriga F. Arsenic increased lipid peroxidation in rat tissues by a mechanism independent of glutathione levels. Environ Health Perspect. 1995; 103: 85-88.

Saha B. Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol 2006; 1: 68-71

Steinmaus C, Carrigan K, Kalman D, Atallah R, Yuan Y, Smith AH. Dietary intake and arsenic methylation in a U.S. population. Environ Health Perspect. 2005; 113: 1153-59.

Tabassum NE. Effect of alpha-lipoic acid on the removal of arsenic from arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol. 2006; 1: 27-32.

Tondel M, Rahman M, Magnuson A, Chowdhury IA, Faruquee MH, Ahmad SA. The relationship of arsenic levels in drinking water and the prevalence rate of skin lesions in Bangladesh. Environ Health Perspect. 1999; 107: 727-29.

Umar BU. Effect of hexane extract of spinach in the removal of arsenic from rat. Bangladesh J Pharmacol. 2007; 2: 27-34.

Wang TS, Huang H. Active oxygen species are involved in the induction of micronuclei by arsenite in XRS-5 cells. Mutagenesis 1994; 9: 253-57.

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How to Cite

Rahman, M. F., and M. Misbahuddin. “Effect of Folic Acid and Tetrahydrofolate on Tissue Arsenic Level in Rat”. Bangladesh Journal of Pharmacology, vol. 5, no. 1, May 2010, pp. 25-29, doi:10.3329/bjp.v5i1.4909.



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