Study of an endophytic fungus from Aquilaria malaccensis Lamk


  • Mohammad Shoeb Department of Chemistry, University of Dhaka, Dhaka 1000
  • Shahanara Begum Department of Chemistry, University of Dhaka, Dhaka 1000
  • Nilufar Nahar Department of Chemistry, University of Dhaka, Dhaka 1000



Aquilaria malaccensis, Antibacterial, Coumarin, Endophytic, HPLC


Nine endophytic fungal strains labeled as AL-1, AL-2, AS-1, AS-2, AS-3, AS-4, AR-1, AR-2 and AR-4 were isolated from the medicinal plant Aquilaria malaccensis Lamk. Three compounds 1,7-dihydroxy-3-methoxyanthraquinone, propyl p-methoxy phenyl ether and  6-methoxy-7-O-(p-methoxyphenyl)-coumarin were isolated from the ethyl acetate extract of the strain AL-2 by silica gel column chromatography and HPLC. The structures of isolated compounds were elucidated by spectroscopic techniques i.e. UV, IR, 1D and 2D NMR. Extracts of the strain AL-2 were assessed for antibacterial activities against Gram positive and Gram negative bacteria and general toxicity against brine shrimp lethality assay. The ethyl acetate extract showed only mild activity against two Gram positive bacteria (Bacillus cereus and Staphylococcus aureus).


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Author Biographies

Mohammad Shoeb, Department of Chemistry, University of Dhaka, Dhaka 1000

Associate Professor

Nilufar Nahar, Department of Chemistry, University of Dhaka, Dhaka 1000



Aly AH, Edrada-Ebel RA, Wray V, Müller WEG, Kozytska S, Hentschel U, Proksch P, Ebel R. Bioactive metabolites from the endophytic fungus Ampelomyces sp. isolated from the medicinal plant Urospermum picroides. Phytochemistry 2008; 69: 1716-25.

Atta-ur-Rahman, Choudhary MI, Thomsen WJ. Bioassay techniques for drug development. Harwood Academic Publishers, 1999, pp 16-17.

Debbab A, Aly AH, Edrada-Ebel RA, Wray V, Müller WEG, Totzke F, Zirrgiebel U, Schächtele C, Kubbutat MHG, Lin WH, Mosaddak M, Hakiki A, Proksch P, Ebel R. Bioactive metabolites from endophytic fungus Stemphylium globuliferum isolated from Mentha pulegium. J Nat Prod. 2009; 72: 626-31.

Kwak JH, Lee KB, Schmitz, FJ. Four new coumarin derivatives from Artemisia keiskeana. J Nat Prod. 2001; 64: 1081-83.

Meyer BN, Ferrigni RN, Putnam JE, Jacobsen LB, Nichols DE, Mclaughlin JL. Brine shrimp- A convenient general bioassay for active plants constituents. Planta Med. 1982; 45: 31-34.

Petrini O. Fungal endophytes of tree leaves. In: Microbial ecology of leaves. New York, Springer-Verlag, 1991, p 179.

Schulz B, Wanke U, Drager S, Aust HJ. Endophytes from herbaceous plants and shrubs: Effectiveness of surface sterilization methods. Mycological Res. 1993; 97: 1447-50.

Strobel G, Yang X, Sears J, Kramer R, Sidhu RS, Hess WM. Taxol from Pestalotiopsis microspora, and endophytic fungus of Taxus wallachiana. Microbiology 1996; 142: 435-40.

Strobel GA, Miller RV, Miller C, Condron M, Teplow DB, Hess WM. Cryptocandin, a potent antimycotic from the endophytic fungus Cryptosporiopsis cf. quercina. Microbiology. 1999; 145: 1919-26.

Strobel G, Daisy B, Castillo U, Harper J. Natural products from engophytic microorganisms. J Nat Prod. 2004; 67: 257-68.

Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc. 1971; 93: 2325-27.

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How to Cite

Shoeb, M., S. Begum, and N. Nahar. “Study of an Endophytic Fungus from Aquilaria Malaccensis Lamk”. Bangladesh Journal of Pharmacology, vol. 5, no. 1, May 2010, pp. 21-24, doi:10.3329/bjp.v5i1.4687.



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