In vitro antiviral activity of BanLec against herpes simplex viruses type 1 and 2

Authors

  • Arisha Taj Mahaboob Batcha Research Scholar, Department of Biochemistry, Dr.N.G.P Arts and Science College, Coimbatore, Tamil Nadu.
  • Ashish Wadhwani Assistant Professor and Head, 2Department of Pharmaceutical Biotechnology, JSS Academy of Higher Education & Research, JSS College of Pharmacy, Ooty-643001, Tamil Nadu, India. https://orcid.org/0000-0001-8102-9486
  • Gowri Subramaniam Professor, Department of Biochemistry,Dr.N.G.P Arts and Science College, Coimbatore, Tamil Nadu. – 641048,India https://orcid.org/0000-0001-6725-4492

DOI:

https://doi.org/10.3329/bjp.v15i1.42320

Keywords:

Antiviral, BanLec, Herpes simplex, Musa paradisiaca

Abstract

The present study evaluates the antiviral activity of banana lectin (BanLec) against herpes simplex virus type 1 and 2 (HSV-1 and HSV-2). Lectin was isolated from the ripen pulp of bananas (Musa paradisiaca). The study showed that lectin exhibited hemagglutination activity towards human erythrocytes A, B, AB and O group. The molecular weight of BanLec using SDS gel-electrophoresis was found to be 14,000-30,000 Da. Cytotoxicity of BanLec on the Vero cell lines showed an inhibitory concentration of 172.7 µg/mL. BanLec was virucidal and showed no cytotoxicity at the concentration tested. The lectin showed a dose-dependent antiviral activities, inhibiting HSV-1 by 16.0 µg/mL with selectivity index 10.8 and HSV-2 inhibition by 67.7 µg/mL with selectivity index 2.6. These results corroborate that BanLec could be a rich source of potential antiviral compound for HSV-1 when compared to HSV-2.

Video Clip of Methodology:

Hemaglutination test: 11 min 29 sec:   Click to watch

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Published

2020-03-08

How to Cite

Mahaboob Batcha, A. T., A. Wadhwani, and G. Subramaniam. “In Vitro Antiviral Activity of BanLec Against Herpes Simplex Viruses Type 1 and 2”. Bangladesh Journal of Pharmacology, vol. 15, no. 1, Mar. 2020, pp. 11-18, doi:10.3329/bjp.v15i1.42320.

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Section

Research Articles