α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots

Authors

  • Songül Karakaya Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240, Turkey http://orcid.org/0000-0002-3268-721X
  • Hilal Özbek Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240, Turkey http://orcid.org/0000-0002-2378-1896
  • Sefa Gözcü Department of Pharmacognosy, Faculty of Pharmacy, Erzincan University, Erzincan 24100, Turkey http://orcid.org/0000-0002-0735-4229
  • Zühal Güvenalp Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240, Turkey http://orcid.org/0000-0002-8803-8147
  • Hafize Yuca Department of Pharmacognosy, Faculty of Pharmacy, Atatürk University, Erzurum 25240, Turkey
  • Hayri Duman Department of Biology, Faculty of Science, Gazi University, Ankara 06500, Turkey
  • Cavit Kazaz Department of Chemistry, Faculty of Science, Atatürk University, Erzurum 25240, Turkey http://orcid.org/0000-0002-5249-0895
  • Ceyda Sibel Kiliç Department of Pharmaceutical Botany, Faculty of Pharmacy, Ankara University, Ankara 06100, Turkey

DOI:

https://doi.org/10.3329/bjp.v13i1.33668

Keywords:

α-Amylase, Ferulago blancheana, Ferulago pachyloba, Ferulago trachycarpa, α-Glucosidase

Abstract

Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC50 values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC50 = 4.9 mg/mL). Grandivitinol (IC50 = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species.

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Published

2018-02-05

How to Cite

Karakaya, S., H. Özbek, S. Gözcü, Z. Güvenalp, H. Yuca, H. Duman, C. Kazaz, and C. S. Kiliç. “α-Amylase and α-Glucosidase Inhibitory Activities of the Extracts and Constituents of Ferulago Blancheana, F. Pachyloba and F. Trachycarpa Roots”. Bangladesh Journal of Pharmacology, vol. 13, no. 1, Feb. 2018, pp. 35-40, doi:10.3329/bjp.v13i1.33668.

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Section

Research Articles