Estimation of vitamin B12 in plasma by High Performance Liquid Chromatography

Authors

  • Fahmida Hasnat Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000
  • Habiba Akhter Bhuiyan Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000
  • Mir Misbahuddin Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000 http://orcid.org/0000-0003-4667-1005

DOI:

https://doi.org/10.3329/bjp.v12i3.33108

Keywords:

High Performance Liquid Chromatography, Plasma, Vitamin B12

Abstract

High performance liquid chromatography was used to develop and validate the detection of vitamin B12 in blood plasma sample. The mobile phase consists of a mixture of 0.025% trifluroacetic acid in deionized water and 30% methanol. The mixture used was adjusted to pH 2.9 and the flow rate was adjusted to 0.5 mL/min. The separation was achieved using C18 column maintained at 30ºC temperature and detection of vitamin B12 was conducted at maximum wavelength 230 nm.

Video Clip of Methodology:

Estimation of vitamin B12 in plasma by HPLC: 4 min 29 sec   Full Screen   Alternate

Downloads

Download data is not yet available.
Abstract
2873
Download
2448

Author Biography

Mir Misbahuddin, Department of Pharmacology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000

Professor

Cell: 880-1849396067; email: dgdabd@gmail.com

ORCHID ID: 0000-0003-4667-1005

Google Scholar Citation Index

 

References

Gentili A, Caretti F, D'Ascenzo G, Marchese S, Perret D, Di Corcia D, Rocca LM. Simultaneous determination of water?soluble vitamins in selected food matrices by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom. 2008; 22: 2029-43.

Girdwood RH. Rapid estimation of the serum vitamin B12 level by a microbiological method. BMJ. 1954; 2(4894): 954-56.

Giorgi MG, Howland K, Martin C, Bonner B. A novel HPLC method for the concurrent analysis and quantitation of seven water-soluble vitamins in biological fluids (plasma and urine): A validation study and application. Sci World J. 2012.

Hancewicz TM, Petty C. Quantitative analysis of vitamin a using Fourier transform Raman spectroscopy. Spectrochim Acta A Mol Spectrosc. 1995; 51: 219398.

Heudi O, Kilinc T, Fontannaz P. Separation of water-soluble vitamins by reversed-phase high performance liquid chromatography with ultraviolet detection: Application to polyvitaminated premixes. J Chromatogr A. 2005; 1070: 49-56.

Karmi O, Zayed A, Baraghethi S, Qadi M, Ghanem R. Measurement of vitamin B12 concentration: A review on available methods. IIOAB J. 2011; 2: 23-32.

Klejdus B, Petrlová J, Pot?il D, Adam V, Mikelová R, Vacek J, Kizek R, Kubá? V., Simultaneous determination of water-and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection. Anal Chim Acta. 2004; 520: 57-67.

Liu BS, Gao J, Yang GL. Determination of vitamin B12 concentration by fluorescence quenching with acridine orange-rhodamine 6G energy transfer system. Guang Pu Xue Yu Guang Pu Fen Xi. 2005; 25: 108082.

Mayer E, Gardiner DJ, Hester RE. Resonance Raman spectra of vitamin B12 and dicyanocobalamin. Biochim Biophys Acta Gen Sub. 1973; 297: 568-70.

Sami R, Li Y, Qi B, Wang S, Zhang Q, Han F, Ma Y, Jing J, Jiang L. HPLC analysis of water-soluble vitamins (B2, B3, B6, B12, and C) and fat-soluble vitamins (E, K, D, A, and ?-carotene) of okra (Abelmoschus esculentus). J Chem. 2014.

Shabangi M, Sutton JA. Separation of thiamin and its phosphate esters by capillary zone electrophoresis and its application to the analysis of water-soluble vitamins. J Pharm Biomed Anal. 2005; 38: 66-71.

Shaik MM, Gan SH. Rapid resolution liquid chromatography method development and validation for simultaneous determination of homocysteine, vitamins B6, B9, and B12 in human serum. Indian J Pharmacol. 2013; 45: 159-67.

Song Z, Hou S. Sub-picogram determination of vitamin B12 in pharmaceuticals and human serum using flow injection with chemiluminescence detection. Anal Chim Acta. 2003; 488: 7179.

Stefova M, Stafilov T, Stojanosk, K, CepreganovaKrstic B. Determination of vitamin B12 in multivitamin tablets by high performance liquid chromatography. Anal Lett. 1997; 30: 2723-31.

Yin C, Cao Y, Ding S, Wang Y. 2008. Rapid determination of water-and fat-soluble vitamins with microemulsion electrokinetic chromatography. J Chromatogr A. 2008; 1193: 172-77.

Downloads

Published

2017-07-12

How to Cite

Hasnat, F., H. A. Bhuiyan, and M. Misbahuddin. “Estimation of Vitamin B12 in Plasma by High Performance Liquid Chromatography”. Bangladesh Journal of Pharmacology, vol. 12, no. 3, July 2017, pp. 251-5, doi:10.3329/bjp.v12i3.33108.

Issue

Section

Visual Experiment