Centipede Scolopendra suppresses cell growth in human epidermoid carcinoma cell A431

Keywords: Centipede Scolopendra, EGFR, A431, MMP2, MMP9

Abstract

The aim of this study was to examine the anti-proliferation and anti-migration effect of Centipede Scolopendra extracts (CSE) on human epidermoid carcinoma cells (high-EGFR expression) A431 and elucidate the underlying signaling mechanisms. MTT and colony formation assays were used. Migration and invasion potential of A431 cells were examined by wound-healing assays and matrigel invasion chamber assays. To investigate the underlying molecular mechanisms, we used ELISA to analyze the expression of EGF, Western blotting to analyze the expression of MMP2 (matrix metalloproteinase 2), MMP9 and EGFR, PCR to analyze the mRNA expression of EGFR pretreated with CSE. The results showed that CSE effectively inhibited the proliferation of A431. Furthermore, CSE-mediated cell cycle arrest in S phase. We also observed that CSE treatment led to down-regulation of MMP2 and MMP9 and suppress the migration and invasion in A431. CSE exerted its anti-proliferation and anti-migration by targeting EGFR and related metastasis factors, thus could be a useful therapeutic candidate for high-EGFR expression cancer intervention.

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References

Cao YJ, He X, Wang N, He LC. Effects of imperatorin, the active component from Radix Angelicae (Baizhi), on the blood pressure and oxidative stress in 2K,1C hypertensive rats. Phytomedicine 2013; 20: 1048-54.

Goldman B, Mach A, Wurzel J. Epidermal growth factor promotes a cardiomyoblastic phenotype in human fetal cardiac myocytes. Exp Cell Res. 1996; 228: 237-45.

Huang SM, Harari PM. Epidermal growth factor receptor inhibition in cancer therapy: Biology, rationale and preliminary clinical results. Invest New Drugs. 1999; 17: 259-69.

Hynes NE. ErbB2: From an EGFR Relative to a central target for cancer therapy. Cancer Res. 2016; 76: 3659-62.

King KL, Cidlowski JA. Cell cycle regulation and apoptosis. Annu Rev Physiol. 1998; 60: 601-17.

Lee JH, Kim IW, Kim SH, Kim MA, Yun EY, Nam SH, Ahn MY, Kang D, Hwang JS. Anticancer activity of the antimicrobial peptide scolopendrasin VII derived from the Centipede, Scolopendra subspinipes mutilans. J Microbiol Biotechnol. 2015; 25: 1275-80.

Li J, Li Y, Feng ZQ, Chen XG. Anti-tumor activity of a novel EGFR tyrosine kinase inhibitor against human NSCLC in vitro and in vivo. Cancer Lett. 2009; 279: 213-20.

Liu ZC, Zhang R, Zhao F, Chen ZM, Liu HW, Wang YJ, Jiang P, Zhang Y, Wu Y, Ding JP, Lee WH. Venomic and transcriptomic analysis of Centipede Scolopendra subspinipes dehaani. J Proteome Res. 2012; 11: 6197-212.

Ma WN, Zhang DD, Zheng L, Zhan YZ, Zhang YM. Potential roles of Centipede Scolopendra extracts as a strategy against EGFR-dependent cancers. Am J Transl Res. 2015; 7: 39-52.

Magkou C, Nakopoulou L, Zoubouli C, Karali K, Theohari I, Bakarakos P, Giannopoulou I. Expression of the epidermal growth factor receptor (EGFR) and the phosphorylated EGFR in invasive breast carcinomas. Breast Cancer Res. 2008; 10: R49.

Merlino GT, Xu YH, Ishii S, Clark AJ, Semba K, Toyoshima K, Yamamoto T, Pastan I. Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells. Science 1984; 224: 417-19.

Nicholson RI, Gee JM, Harper ME. EGFR and cancer prognosis. Eur J Cancer. 2001; 37: S9-15.

Oliveira S, van Bergen en Henegouwen PM, Storm G, Schiffelers RM. Molecular biology of epidermal growth factor receptor inhibition for cancer therapy. Expert Opin Biol Ther. 2006; 6: 605-17.

Shimamura M, Hazato T, Ashino H, Yamamoto Y, Iwasaki E, Tobe H, Yamamoto K, Yamamoto S. Inhibition of angiogenesis by humulone, a bitter acid from beer hop. Biochem Bioph Res Co. 2001; 289: 220-24.

Sirkisoon SR, Carpenter RL, Rimkus T, Miller L, Metheny-Barlow L, Lo HW. EGFR and HER2 signaling in breast cancer brain metastasis. Front Biosci (Elite Ed). 2016; 8: 245-63.

Undheim EA, King GF. On the venom system of centipedes (Chilopoda), a neglected group of venomous animals. Toxicon 2011; 57: 512-24.

Wells A. EGF receptor. Int J Biochem Cell Biol. 1999; 31: 637-43.

Woodburn JR. The epidermal growth factor receptor and its inhibition in cancer therapy. Pharmacol Ther. 1999; 82: 241-50.

Yarden Y, Sliwkowski MX. Untangling the ErbB signalling network. Nat Rev Mol Cell Biol. 2001; 2: 127-37.

Zhan YZ, Zhang YM, Liu CC, Zhang J, Smith WW, Wang N, Chen YN, Zheng L, He LC. A novel taspine derivative, HMQ1611, inhibits breast cancer cell growth via estrogen receptor alpha and EGF receptor signaling pathways. Cancer Prev Res (Phila). 2012; 5: 864-73.

Published
2017-08-20
How to Cite
Zheng, L., H. He, X. Shen, and Y. Sun. “Centipede Scolopendra Suppresses Cell Growth in Human Epidermoid Carcinoma Cell A431”. Bangladesh Journal of Pharmacology, Vol. 12, no. 3, Aug. 2017, pp. 299-07, doi:10.3329/bjp.v12i3.32525.
Section
Research Articles