Detection of cytosolic tRNA in mammal by Northern blot analysis

Authors

  • Alshammari Fanar Hamad Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749
  • Hye-Young Jeong Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749
  • Jong-Hun Han Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749
  • Irfan Ahmad Rather Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749

DOI:

https://doi.org/10.3329/bjp.v12i3.32501

Keywords:

Northern Blot, RNA, rRNA

Abstract

Out of entire cascade of technologies and strategies, Northern blot assay remains the most preferential approach for immediate and accurate evaluation of expressed RNA species. However, an abundance of tRNAs species under physiological conditions compared to other small RNAs makes it difficult to accurately evaluate their transcriptional alterations through traditional Northern blot assay. Here, we describe an efficient protocol for detecting subtle alterations in tRNA species in mammals by a modified Northern blot assay. This report also compares the chemical versus UV-based crosslinking of tRNA species to the surface of solid supports.

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Author Biography

Irfan Ahmad Rather, Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712749

Assistant Professor

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References

Alwine JC, Kemp DJ, Stark GR. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA. 1977; 74: 5350-54.

Gesteland RF, Cech TR, Atkins JF. (eds.) The RNA World. 3rd ed. Cold Spring Harbor Laboratory Press. 2006.

Laneve P, Giangrande A. Enhanced Northern blot detection of small RNA species in Drosophila melanogaster. J Vis Exp. 2014; 9: e51814.

Wei Y. Northern blot of tRNA in yeast. Bio-protocol. 2013; 3: e464.

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Published

2017-07-08

How to Cite

Hamad, A. F., H.-Y. Jeong, J.-H. Han, and I. A. Rather. “Detection of Cytosolic TRNA in Mammal by Northern Blot Analysis”. Bangladesh Journal of Pharmacology, vol. 12, no. 3, July 2017, pp. 243-50, doi:10.3329/bjp.v12i3.32501.

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Section

Visual Experiment