Visual demonstration of transmission electron microscopy for intracellular observation of a single bacterial cell
Keywords:Bacteria, Intracellular, Transmission electron microscopy
Transmission electron microscopy (TEM) has a profound impact on knowledge and understanding of bacteria and other microbial populations. The 1000-fold improvement in resolution provided by TEM has allowed visualization of bacterial cells. Of all the types of microscopy exploited to date, TEM is the one with the most advantageous resolution limit and therefore it is a very efficient technique for deciphering the cell architecture and relating it to function. This visual experiment aims to provide an overview of the most important technique, TEM that we can apply to a biological sample, tissue or cells, to observe it with an internal structure, from the most conventional to the latest generation. Processes and concepts are defined, and the advantages and disadvantages of the TEM technique are assessed along with the image and information that we can obtain by using each one of them.
Video Clips of Methodology:
Agar AW, Alderson RH, Chescoe D, Glauert AM. Principles and practice of electron microscope operation. In: Practical methods in electron microscopy. Glauert AM (ed). London, Elsevier Science and Technology, 1974.
Cortadellas N, Garcia A, Fernández E. Transmission electron microscopy in cell biology: Sample preparation techniques and image information. Handbook of instrumental techniques from CCiTUB. TEM Cell Biol. 2010, pp 1-11.
Glauert AM, Lewis PR. Biological specimen preparation for transmission electron microscopy. New Jersey, Princeton University Press, 1988.
Grossart HP, Steward GF, Martinez J, Azam F. A simple, rapid method for demonstrating bacterial flagella. Appl Environ Microbiol. 2000; 66: 363236.
Hayat MA. Principles and techniques of electron microscopy: Biological applications. Cambridge, Cambridge University Press, 2000.
Kodaka H, Armfield AY, Lombard GL, Dowell VR. Practical procedure for demonstrating bacterial flagella. J Clin Microbiol. 1992; 16: 948-52.
Mascorro JA, Bozzola JJ. Processing biological tissues for ultrastructural study. In: Electron microscopy. Methods and protocols. Methods in Molecular Biology. John Kuo (ed). Human Press, 2007.
Porter JR, Thomulka KW, Smith RA. Demonstrating bacterial flagella. Am Biol Technol. 1992; 54: 108-11.
Ruska E. The development of the electron microscope and of electron microscopy. Biosci Rep. 1987; 7: 607-29.
Shukla S. Studying antimicrobial-induced morphostructural damage of bacteria by Scanning Electron Microscope. Bangladesh J Pharmacol. 2015; 10: 870-74.
Vale FF, Correia, AC, Matos, B, Moura Nunes JF, Alves de Matos AP. Applications of transmission electron microscopy to virus detection and identification. Microscopy: Science, technology, applications and education. Méndez-Vilas A, Díaz J (eds). 2010, pp 128-36.
Willey J, Sherwood L, Woolverton C. Prescott, Harley and Klein's Microbiology. 7th ed. London, McGraw-Hill, 2008.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).