Taraxerol protects the human hepatic L02 cells from hydrogen peroxide-induced apoptosis


  • Xiang-Yang Yao School of Food and Biology Engineering, Bengbu University, Bengbu 233030 http://orcid.org/0000-0003-2956-6713
  • Qin Bai School of Food and Biology Engineering, Bengbu University, Bengbu 233030




Apoptosis, Human hepatic L02 cell, Hydrogen peroxide, Taraxerol


Taraxerol is known to exhibit anti-inflammatory and anti-cancer activity. However, cytoprotective effect of taraxerol on hepatocytes has not been reported. In the present study, we investigated the hepatoprotective effect of taraxerol in the human hepatic L02 cells injured by hydrogen peroxide (H2O2). Taraxerol decreased H2O2-induced cell viability loss and lactate dehydrogenase release. Taraxerol also inhibited H2O2-induced cell apoptosis. Further, taraxerol attenuated H2O2-induced increase in cleaved-caspase-3 and cleaved-PARP. H2O2-activated p38 and JNK were also inhibited by taraxerol. These data suggest that taraxerol could protect the L02 cells against H2O2-induced apoptosis via suppression of p38 and JNK. Taraxerol may be an effective protective agent against oxidative stress-induced liver injury.

Video Clip of Methodology: 6 min 20 sec   Full Screen    Alternate


Download data is not yet available.
994 Read


Brewer TF, Garcia FJ, Onak CS, Carroll KS, Chang CJ. Chemical approaches to discovery and study of sources and targets of hydrogen peroxide redox signaling through NADPH oxidase proteins. Annu Rev Biochem. 2015; 84: 765-90.

Chen MC, Ye YY, Ji G, Liu JW. Hesperidin upregulates heme oxygenase-1 to attenuate hydrogen peroxide-induced cell damage in hepatic L02 cells. J Agric Food Chem. 2010; 58: 3330-35.

Cory S, Adams JM. Killing cancer cells by flipping the Bcl-2/Bax switch. Cancer Cell. 2005; 8: 5-6.

Gough DR, Cotter TG. Hydrogen peroxide: A Jekyll and Hyde signalling molecule. Cell Death Dis. 2011; 2: e213.

Groeger G, Quiney C, Cotter TG. Hydrogen peroxide as a cell-survival signaling molecule. Antioxid Redox Signal. 2009; 11: 2655-71.

Guicciardi ME, Gores GJ. Apoptosis as a mechanism for liver disease progression. Semin Liver Dis. 2010; 30: 402-10.

Huang C, Lin Y, Su H, Ye D. Forsythiaside protects against hydrogen peroxide-induced oxidative stress and apoptosis in PC12 cell. Neurochem Res. 2015; 40: 27-35.

Jang DS, Cuendet M, Pawlus AD, Kardono LB, Kawanishi K, Farnsworth NR, Fong HH, Pezzuto JM, Kinghorn AD. Potential cancer chemopreventive constituents of the leaves of Macaranga triloba. Phytochemistry 2004; 65: 345-50.

Li G, Kang J, Yao X, Xin Y, Wang Q, Ye Y, Luo L, Yin Z. The component of green tea, L-theanine protects human hepatic L02 cells from hydrogen peroxide-induced apoptosis. Eur Food Res Technol. 2011; 233: 427-35.

Li J, Li W, Huang M, Zhang X. Fisetin, a dietary flavonoid induces apoptosis via modulating the MAPK and PI3K/Akt signalling pathways in human osteosarcoma (U-2 OS) cells. Bangladesh J Pharmacol. 2015; 10: 820-29.

Liu Y, E Q, Zuo J, Tao Y, Liu W. Protective effect of cordyceps polysaccharide on hydrogen peroxide-induced mitochondrial dysfunction in HL-7702 cells. Mol Med Rep. 2013; 7: 747-54.

Mantzaris MD, Bellou S, Skiada V, Kitsati N, Fotsis T, Galaris D. Intracellular labile iron determines H2O2-induced apoptotic signaling via sustained activation of ASK1/JNK-p38 axis. Free Radic Biol Med. 2016; 97: 454-65.

McCubrey JA, Lahair MM, Franklin RA. Reactive oxygen species-induced activation of the MAP kinase signaling pathways. Antioxid Redox Signal. 2006; 8: 1775-89.

Sangeetha KN, Shilpa K, Jyothi Kumari P, Lakshmi BS. Reversal of dexamethasone induced insulin resistance in 3T3L1 adipocytes by 3beta-taraxerol of Mangifera indica. Phytomedicine 2013; 20: 213-20.

Sangeetha KN, Sujatha S, Muthusamy VS, Anand S, Nithya N, Velmurugan D, Balakrishnan A, Lakshmi BS. 3beta-taraxerol of Mangifera indica, a PI3K dependent dual activator of glucose transport and glycogen synthesis in 3T3-L1 adipocytes. Biochim Biophys Acta. 2010; 1800: 359-66.

Setzer WN, Shen X, Bates RB, Burns JR, McClure KJ, Zhang P, Moriarity DM, Lawton RO. A phytochemical investigation of Alchornea latifolia. Fitoterapia 2000; 71: 195-98.

Sharma K, Zafar R. Occurrence of taraxerol and taraxasterol in medicinal plants. Pharmacogn Rev. 2015; 9: 19-23.

Takasaki M, Konoshima T, Tokuda H, Masuda K, Arai Y, Shiojima K, Ageta H. Anti-carcinogenic activity of taraxacum plant. II. Biol Pharm Bull. 1999; 22: 606-10.

Tsao CC, Shen YC, Su CR, Li CY, Liou MJ, Dung NX, Wu TS. New diterpenoids and the bioactivity of Erythrophleum fordii. Bioorg Med Chem. 2008; 16: 9867-70.

Veal E, Day A. Hydrogen peroxide as a signaling molecule. Antioxid Redox Signal. 2011; 15: 147-51.

Wang H, Xue Z, Wang Q, Feng X, Shen Z. Propofol protects hepatic L02 cells from hydrogen peroxide-induced apoptosis via activation of extracellular signal-regulated kinases pathway. Anesth Analg. 2008; 107: 534-40.

Xing HY, Liu Y, Chen JH, Sun FJ, Shi HQ, Xia PY. Hyperoside attenuates hydrogen peroxide-induced L02 cell damage via MAPK-dependent Keap(1)-Nrf(2)-ARE signaling pathway. Biochem Bioph Res Co. 2011; 410: 759-65.

Yao X, Li G, Bai Q, Xu H, Lü C. Taraxerol inhibits LPS-induced inflammatory responses through suppression of TAK1 and Akt activation. Int Immunopharmacol. 2013; 15: 316-24.



How to Cite

Yao, X.-Y., and Q. Bai. “Taraxerol Protects the Human Hepatic L02 Cells from Hydrogen Peroxide-Induced Apoptosis”. Bangladesh Journal of Pharmacology, vol. 12, no. 2, Apr. 2017, p. Available Online: Apr 20, 2017, doi:10.3329/bjp.v12i2.30985.



Research Articles