MTT assay to evaluate the cytotoxic potential of a drug

Authors

DOI:

https://doi.org/10.3329/bjp.v12i2.30892

Keywords:

Cell viability, Cytotoxicity, Formazan, MTT assay

Abstract

Quantification of cell viability and proliferation form the fundamental for numerous in vitro assays in response to external factors. An MTT assay is a colorimetric assay based on assessing the cell metabolic activity. A549 Lung adenocarcinoma cell line was used to see the cytotoxic potential of a new drug for initial screening of apoptosis or necrosis. The biochemical mechanism behind the MTT assay involves NAD(P)H-dependent cellular oxidoreductase enzyme that converts the yellow tetrazolium MTT [3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] into insoluble (E,Z)-5-(4,5-dimethylthiazol-2-yl)-1,3-diphenylformazan (formazan). The formed formazan can be dissolved with dimethyl sulfoxide (DMSO) to give a purple color with characteristic absorption at 540 nm. Intensity of purple color is directly proportional to the cell number and thus indicating the cell viability.

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Author Biographies

Ashutosh Bahuguna, Department of Biotechnology, College of Engineering, Daegu University, Daegu

Post-doc

Mob: +82-10-48321341

Imran Khan, Department of Biotechnology, College of Engineering, Daegu University, Daegu

PhD scholar

Mobile: +821073549718

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University

Assistant Professor

Sun Chul Kang, Department of Biotechnology, College of Engineering, Daegu University, Daegu

Professor

References

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van Meerloo J, Kaspers GJL, Cloos J. Cell sensitivity assays: The MTT assay. Methods Mol Biol. 2011; 731: 237-45.

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Published

2017-04-08

How to Cite

Bahuguna, A., I. Khan, V. K. Bajpai, and S. C. Kang. “MTT Assay to Evaluate the Cytotoxic Potential of a Drug”. Bangladesh Journal of Pharmacology, vol. 12, no. 2, Apr. 2017, p. Available Online: Apr 8, 2017, doi:10.3329/bjp.v12i2.30892.

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Section

Visual Experiment