Chemical derivatization of pharmaceutical samples prior to Gas-Chromatography and Mass-Spectrometry analysis

Authors

  • Vivek K. Bajpai Department of Applied Microbiology and Biotechnology, Yeungnam University
  • Na-Hyung Kim Department of Oriental Pharmacy, Wonkwang University, Iksan-City, Jeonbuk
  • Kangmin Kim Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si-570-752, Joellabuk-do (Jeonbuk)

DOI:

https://doi.org/10.3329/bjp.v11i4.28588

Keywords:

Chemical derivatization, Gas-Chromatography, Mass-Spectrometry, Pharmaceutical

Abstract

Derivatization is the process by which a compound is chemically changed, that has properties more amenable to a particular analytical method. Some samples analyzed by Gas-chromatography and Mass-spectrometry (GC-MS) require derivatization in order to make them suitable for analysis. Especially the compounds that have poor volatility, poor thermal stability, or that can be adsorbed in the injector will exhibit non-reproducible peak areas, heights, and shapes. Hence, this study focused on step-by-step visual demonstration of chemical derivatization of pharmaceutical samples prior to GC-MS analysis for their better elution and detection.

Video Clip of Methodology:

Part 1: 5 min 8 sec   Full Screen   Alternate

Part 2: 6 min 13 sec   Full Screen   Alternate

Part 3: 53 sec   Full Screen   Alternate

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Author Biographies

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University

Assistant Professor

Na-Hyung Kim, Department of Oriental Pharmacy, Wonkwang University, Iksan-City, Jeonbuk

Professor

References

Farag MA, Rasheed DM, Kamal IM. Volatiles and primary metabolites profiling in two Hibiscus sabdariffa (roselle) cultivars via headspace SPME-GC-MS and chemometrics. Food Res Int. 2015; 78: 327-35.

Japelt KB, Nielsen NJ, Wiese S, Christensen JH. Metabolic fingerprinting of Lactobacillus paracasei: A multi-criteria evaluation of methods for extraction of intracellular metabolites. Anal Bioanal Chem. 2015; 407: 6095-104.

Knapp DR. Handbook of analytical derivatization reaction. Vol. 10. New York, Wiley and Sons, 1979, p 10.

Kuhnel E, Laffan DDP, Lloyd-Jones GC, Martinez del Campo T, Shepperson IR, Slaughter JL. Mechanism of methyl esterification of carboxylic acids by trimethylsilyldiazomethane. Angewandte Chem Int. 2007; 46: 7075-78.

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Published

2016-10-01

How to Cite

Bajpai, V. K., N.-H. Kim, and K. Kim. “Chemical Derivatization of Pharmaceutical Samples Prior to Gas-Chromatography and Mass-Spectrometry Analysis”. Bangladesh Journal of Pharmacology, vol. 11, no. 4, Oct. 2016, pp. 852-5, doi:10.3329/bjp.v11i4.28588.

Issue

Section

Visual Experiment