Isolation and purification of plant secondary metabolites using column-chromatographic technique

Authors

  • Vivek K. Bajpai Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749 http://orcid.org/0000-0003-0423-6195
  • Rajib Majumder Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749
  • Jae Gyu Park Pohang Technopark, Pohang Center for Evaluation of Biomaterials, 394, Jigok-ro, Nam-gu, Pohang, Gyeongbuk

DOI:

https://doi.org/10.3329/bjp.v11i4.28185

Keywords:

Column chromatography, Metabolite, Plant

Abstract

Chromatographic techniques have significant role in natural products chemistry as well as contribute dramatically in the discovery of novel and innovative compounds of pharmaceutical and biomedical importance. This study focused on step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatographic involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions; and e) Analysis of each fractions using thin layer chromatography. However, depending on nature of research, compounds can be further purified using high performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectral analyses.

Video Clips of Methodology:

Part 1: 3 min 45 sec   Full Screen   Alternate

Part 2: 6 min 21 sec   Full Screen   Alternate

Part 3: 4 min 45 sec   Full Screen   Alternate

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Author Biographies

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749

Assistant Professor

Google Scholar Citations

Rajib Majumder, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749

Researcher

Jae Gyu Park, Pohang Technopark, Pohang Center for Evaluation of Biomaterials, 394, Jigok-ro, Nam-gu, Pohang, Gyeongbuk

Scientist

References

Bajpai VK, Kang SC. Isolation and characterization of biologically active secondary metabolites from Metasequoia glyptostroboides Miki ex Hu. J Food Safety. 2011; 31: 276-83.

El-Shemy HA, Aboul-Enein AM, Aboul-Enein KM, Fujita K. Willow leaves extracts contain antitumor agents effective against three cell types. PLoS ONE 2007; 2: e178.

Li AN, Li S, Zhang YJ, Xu XR, Chen YM, Li HB. Resources and biological activities of natural polyphenols. Nutrients 2014; 6: 6020-47.

Russell W, Duthie G. Plant secondary metabolites and gut health: The case for phenolic acids. Proc Nutr Soc. 2011; 70: 389-96.

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Published

2016-10-01

How to Cite

Bajpai, V. K., R. Majumder, and J. G. Park. “Isolation and Purification of Plant Secondary Metabolites Using Column-Chromatographic Technique”. Bangladesh Journal of Pharmacology, vol. 11, no. 4, Oct. 2016, pp. 844-8, doi:10.3329/bjp.v11i4.28185.

Issue

Section

Visual Experiment