Effects of Alchemilla mollis and Alchemilla persica on the wound healing process

  • Burçin Ergene Öz Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100, Ankara http://orcid.org/0000-0001-6927-6652
  • Mert Ilhan Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara
  • Serkan Ozbilgin Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100, Ankara http://orcid.org/0000-0002-3945-6756
  • Esra Küpeli Akkol Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara
  • Özlem Bahadir Acikara
  • Gülçin Saltan Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100, Ankara
  • Hikmet Keles Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar
  • Ipek Süntar Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara
Keywords: Alchemilla mollis, Alchemilla persica, Anti-inflammatory, Wound healing

Abstract

Alchemilla mollis, is used in traditional medicine for the treatment of wounds and excessive menstruation. Aqueous methanol extracts of A. mollis and A. persica were evaluated for wound healing acivity by using linear incision and circular excision wound models along with hydroxyproline estimation and histopathological analysis. Anti-inflammatory effect was determined according to Whittle method. The extracts prepared from the aerial parts of A. mollis and A. persica exerted significant wound healing activity with the tensile strength values of 39.3% and 33.3%, respectively, and with the contraction values of 51.4% and 43.5%, respectively. Hydroxyproline estimation and histopathological analysis also confirmed the results. The extracts of A. mollis and A. persica showed significant anti-inflammatory activity with the values of 30.6% and 26.6% respectively. These results showed that A. mollis and A. persica possess significant wound healing and anti-inflammatory activities.

Video Clip of Methodology:

Circular excision wound model: 2 min   Full Screen   Alternate

Downloads

Download data is not yet available.
Abstract
1788
Download
842 Read
966 Feature
308

Author Biography

Ipek Süntar, Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler 06330, Ankara
Associate Professor

References

Akbulut S, Bayramoglu MM. The trade and use of some medical and aromatic herbs in Turkey. Stud Ethno-Med. 2013; 7: 67-77.

Baytop T. Therapy with medicinal plants in Turkey: Today and in future. Istanbul, Nobel Publishers, 1999.

D'Agostino M, Dini I, Ramundo E, Senatore F. Flavonoid glycosides of Alchemilla vulgaris L. Phytother Res. 1998; 12: 162-63.

Duckstein SM, Lotter EM, Meyer U, Lindequist U, Stintzing FC. Phenolic constituents from Alchemilla vulgaris L. and Alchemilla mollis (Buser) Rothm. at different dates of harvest. Z. Naturforsch C. 2012; 67: 529-40.

Ergene B, Bahadir Acikara Ö, Bakar F, Saltan G, Nebio?lu S. Anti-oxidant activity and phytochemical analysis of Alchemilla persica Rothm. J Fac Pharm Ankara. 2010; 39: 145-54.

ESCOP Monographs: The scientific foundation for herbal medicinal products. United Kingdom, 2003.

Getie M, Gebre-Mariam T, Rietz R, Neubert RH. Evaluation of the release profiles of flavonoids from topical formulations of the crude extract of the leaves of Dodonea viscosa (Sapindaceae). Pharmazie 2002; 57: 320-22.

Gruenwald J, Brendler T, Jaenicke C. Physicians desk reference for herbal medicines (PDR). USA, Medical Economics Company, 2000.

Kim SJ, Um JY, Lee JY. Anti-inflammatory activity of hyperoside through the suppression of nuclear factor-?B activation in mouse peritoneal macrophages. Am J Chin Med. 2011; 39: 171-81.

Kumarasamy Y, Nahar L, Cox PJ, Jaspars M, Sarker SD. Bio-activity of secoiridoid glycosides from Centaurium erythraea. Phytomedicine 2003; 10: 344-47.

Kupeli Akkol E, Demirel MA, Bahadir Acikara O, Süntar I, Ergene B, Ilhan M, Ozbilgin S, Saltan G, Keles H, Tekin M. Phytochemical analyses and effects of Alchemilla mollis (Buser) Rothm. and Alchemilla persica Rothm. in rat endometriosis model. Arch Gynecol Obstet. 2015; 292: 619-28.

Lodhi S, Pawar RS, Jain AP, Singhai AK. Wound healing potential of Tephrosia purpurea (Linn.) Pers. in rats. J Ethnopharmacol. 2006; 108: 204-10.

Makau JN, Watanabe K, Kobayashi N. Anti-influenza activity of Alchemilla mollis extract: Possible virucidal activity against influenza virus particles. Drug Discov Ther. 2013; 7: 189-95.

Neagu E, Paun G, Albu C, Radu GL. Assessment of acetyl-cholinesterase and tyrosinase inhibitory and anti-oxidant activity of Alchemilla vulgaris and Filipendula ulmaria extracts. J Taiwan Inst Chem E. 2015; 52: 1-6.

Shetty S, Udupa S, Udupa L. Evaluation of anti-oxidant and wound healing effects of alcoholic and aqueous extract of Ocimum sanctum Linn in rats. Evid Based Complement Alternat Med. 2008; 5: 95-101.

Shrivastava R, Cucuat N, John GW. Effects of Alchemilla vulgaris and glycerine on epithelial and myofibroblast cell growth and cutaneous lesion healing in rats. Phytother Res. 2007; 21: 369-73.

Shrivastava R, John GW. Treatment of aphthous stomatitis with topical Alchemilla vulgaris in glycerine. Clin Drug Investig. 2006; 26: 567-73.

Spanos GA, Wrolstad RE. Influence of processing and storage on the phenolic composition of thompson seedless grape juice. J Agric Food Chem. 1990; 38: 1565-71.

Suguna L, Singh S, Sivakumar P, Sampath P, Chandrakasan G. Influence of Terminalia chebula on dermal wound healing in rats. Phytother Res. 2002; 16: 227-31.

Süntar I, Akkol EK, Keles H, Oktem A, Baser KHC, Yesilada E. A novel wound healing ointment: A formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge. J Ethnopharmacol. 2011; 134: 89-96.

Süntar IP, Kupeli Akkol E, Yilmazer D, Baykal T, Kirmizibekmez H, Alper M, Yesilada E. Investigations on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol. 2010; 127: 468-77.

Süntar I, Kupeli Akkol E, Keles H, Yesilada E, Sarker SD, Arroo R, Baykal T. Efficacy of Daphne oleoides subsp. kurdica used for wound healing: Identification of active compounds through bioassay guided isolation technique. J Ethnopharmacol. 2012; 141: 1058-70.

Trendafilova A, Todorova M, Gavrilova A, Vitkova A. Flavonoid glycosides from Bulgarian endemic Alchemilla achtarowii Pawl. Biochem Syst Ecol. 2012; 43: 156-58.

Trendafilova A, Todorova M, Nikolova M, Gavrilova A, Vitkova A. Flavonoid constituents and free radical scavenging activity of Alchemilla mollis. Nat Prod Commun. 2011; 6: 1851-54.

Valentova K, Vrba J, Bancirova M, Ulrichova J, Kren V. Isoquercitrin: Pharmacology, toxicology, and metabolism. Food Chem Toxicol. 2014; 68: 267-82.

Whittle BA. The use of changes in capillary permeability in mice to distinguish between narcotic and non-narcotic analgesics. Br J Pharmacol. 1964; 22: 246-53.

Yarnel E, Abascal K. Multiphasic herbal prescribing for menstruating women. Alternat Complement Ther. 2009; 15: 126-34.

Yesilada E, Kupeli E. Clematis vitalba L. aerial part exhibits potent anti-inflammatory, anti-nociceptive and anti-pyretic effects. J Ethnopharmacol. 2007; 110: 504-15.

Published
2016-06-02
How to Cite
Ergene Öz, B., M. Ilhan, S. Ozbilgin, E. Küpeli Akkol, Özlem Bahadir Acikara, G. Saltan, H. Keles, and I. Süntar. “Effects of Alchemilla Mollis and Alchemilla Persica on the Wound Healing Process”. Bangladesh Journal of Pharmacology, Vol. 11, no. 3, June 2016, pp. 577-84, doi:10.3329/bjp.v11i3.26024.
Section
Research Articles