Enhanced antitumor activity of cucurbitacin B combined with cerulenin in osteosarcoma

Authors

  • Zhe Zhang Zhongnan Hospital of Wuhan University, Wuhan 430071, China
  • Ying Shen Xianning Central Hospital, Xianning 437100, China
  • Jian Wu Xianning Central Hospital, Xianning 437100, China
  • Lin Cai Zhongnan Hospital of Wuhan University, Wuhan 430071, China http://orcid.org/0000-0002-2358-7567

DOI:

https://doi.org/10.3329/bjp.v10i4.24601

Keywords:

Cerulenin, Cucurbitacin B, Osteosarcoma, Tumor

Abstract

In the present study, the effect of cucurbitacin B and cerulenin combination on osteosarcoma was investigated to develop an effective treatment regimen. The IC50 values of cerulenin and cucurbitacin B combination in the proportion of 1:1, 1:2 and 2:1 were  3.5, 7.2 and 8.6 μg/mL, respectively. The combination index (CI) values of <0.95 for inhibition of growth and <0.93 for inhibition of SENP5 expression clearly indicated synergism between the two. The Q-value for combination of 1 μg/mL cerulenin and 1 μg/mL cucurbitacin B was 1.2. AI calculated for tumor tissues treated with a combination of cucurbitacin B and cerulenin (26.2 ± 8.4%) was higher than that of the tissues treated separately with cucurbitacin B (14.5 ± 6.8%) or cerulenin (12.6 ± 7.5%). The AI for untreated tumor tissues was 4.2 ± 1.5%. Thus, the combination of cucurbitacin B and cerulenin can be a promising regimen in the treatment of osteosarcoma.

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Published

2015-11-19

How to Cite

Zhang, Z., Y. Shen, J. Wu, and L. Cai. “Enhanced Antitumor Activity of Cucurbitacin B Combined With Cerulenin in Osteosarcoma”. Bangladesh Journal of Pharmacology, vol. 10, no. 4, Nov. 2015, pp. 956-61, doi:10.3329/bjp.v10i4.24601.

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Section

Research Articles