In vitro antiviral activity of Lactobacillus plantarum using SPF embryonated eggs and hemagglutination assay
Keywords:Antiviral activity, Hemagglutination assay, Lactobacillus plantarum, SPF embryonated egg
Serum pathogen-free (SPF) embryonated egg for culturing virus has three main steps: a) candling of egg, b) inoculation of egg and c) harvesting of inoculated egg. Furthermore, hemagglutination assay consists of only one step. The method is easy to perform. However, this experiment must be performed in a class 2 biosafety cabinet.
Hemagglutination test: 7.3 min
Rather IA, Choi KH, BajpaiVK, Park YH. Antiviral mode of action of Lactobacillus plantarum YML009 on Influenza virus H1N1. Bangladesh J Pharmacol. 2015; 10: 475-82.
Seo BJ, Rather IA, Kumar VJR, Choi UH, Moon MR, Lim JH, Park YH. Evaluation of Leuconostoc mesenteroides YML003 as a probiotic against low-pathogenic avian influenza (H9N2) virus in chickens. J Appl Microbiol. 2012; 113: 163-71.
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