In vitro antiviral activity of Lactobacillus plantarum using SPF embryonated eggs and hemagglutination assay

Authors

DOI:

https://doi.org/10.3329/bjp.v10i3.24475

Keywords:

Antiviral activity, Hemagglutination assay, Lactobacillus plantarum, SPF embryonated egg

Abstract

Serum pathogen-free (SPF) embryonated egg for culturing virus has three main steps: a) candling of egg, b) inoculation of egg and c) harvesting of inoculated egg. Furthermore, hemagglutination assay consists of only one step. The method is easy to perform. However, this experiment must be performed in a class 2 biosafety cabinet.

 

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In vitro antiviral activity test using SPF embryonated eggs:   7.5 min

Hemagglutination test:                                                    7.3 min

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Author Biographies

Rajib Majumder, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan

Graudate student 

Irfan A. Rather, Department of Applied Microbiology and Biotechnology, Gyeongsan Si, Gyeongsan 712 749

Assitant Professor

Google Scholar Citation

Vivek K. Bajpai, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan

Assistant Professor

Google Scholar Citation

Yong-Ha Park, Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, South Korea.

Professor

References

Rather IA, Choi KH, BajpaiVK, Park YH. Antiviral mode of action of Lactobacillus plantarum YML009 on Influenza virus H1N1. Bangladesh J Pharmacol. 2015; 10: 475-82.

Seo BJ, Rather IA, Kumar VJR, Choi UH, Moon MR, Lim JH, Park YH. Evaluation of Leuconostoc mesenteroides YML003 as a probiotic against low-pathogenic avian influenza (H9N2) virus in chickens. J Appl Microbiol. 2012; 113: 163-71.

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Published

2015-08-11

How to Cite

Majumder, R., I. A. Rather, V. K. Bajpai, and Y.-H. Park. “In Vitro Antiviral Activity of Lactobacillus Plantarum Using SPF Embryonated Eggs and Hemagglutination Assay”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, Aug. 2015, pp. 688-91, doi:10.3329/bjp.v10i3.24475.

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Section

Visual Experiment