Evaluation of anticonvulsant, antimicrobial and hemolytic activity of Aitchisonia rosea

  • Shahid Rasool University College of Pharmacy, University of the Punjab, Lahore 54000 http://orcid.org/0000-0003-4489-1362
  • Farrukh Zia Khan University College of Pharmacy, University of the Punjab, Lahore 54000
  • Mobasher Ahmad University College of Pharmacy, University of the Punjab, Lahore 54000
Keywords: Aitchisonia rosea, Anticonvulsant, Antimicrobial, Hemolytic

Abstract

The purpose of this study was to evaluate the anticonvulsant, antimicrobial and hemolytic effect of Aitchisonia rosea. The anticonvulsant effect was studied at doses 400 and 800 mg/kg against pentylenetetrazole, strychnine and picrotoxin-induced seizures in albino mice. The antimicrobial assay was conducted by disc diffusion method and minimum inhibitory concentration. Hemolytic effect was analyzed by reported method. Phenolic compounds present in the n-butanol fraction of the plant were estimated by HPLC. The plant showed maximum response against drug-induced convulsions and provided protection to animals at both doses. It also showed maximum zone of inhibition and highly significant MIC against all bacterial and fungal strains. The plant protected the RBCs from hemolysis. The highest amount of phenolics found was caffeic acid (7.5 ± 0.04).

Downloads

Download data is not yet available.
Abstract
1377
Download
637 Read
714

References

Adeyemi OO, Akindele AJ, Yemitan OK, Fagbo FI. Anticonvulsant, anxiolytic and sedative activities of the aqueous root extract of Securida calonge and pendunculata fresen. J Ethanopharmacol. 2010; 130: 191-95.

Ali SI, Nasir E. Flora of Pakistan. Department of Botany, University of Karachi, Karachi, 1989.

Aslam F, Rasool N, Riaz M, Zubair M, Rizwan K, Abbas M, Bukhari TH, Bukhari IH. Antioxidant, haemolytic activities and GC-MS profiling of Carissa carandas. Inter J Phytomed. 2011; 3: 567-78.

Brain KR, Turner KR. The practical evaluation of phytopharmaceuticals. UK, Wright- Scientehnica, 1975, pp 187-89.

Bum EN, Schmutz M, Meyer C, Rakotonirina A, Bopelet M, Porter C. Anticonvulsant properties of the methanolic extract of Cyperus articulates. J Ethnopharmacol. 2001; 76: 145-50.

Curtis DR, Duggan AW, Felix D, Johnston GAR, McLennan H. Antagonism between bicculline and GABA in the cat brain. Brain Res. 1971; 33: 57-73.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afri J Biotech. 2005; 4: 685-88.

Ergene A, Guler P, Tan S, Mirci S, Hamzaoglu E. Antimicrobial and antifungal activity of Heracleums phondylium subsp. artivinense. Afr J Biotech. 2006; 11: 1087.

Erol NT, Sar? F, Vel??oglu YS. Polyphenols, alkaloids and antioxidant activity of different grades of turkish black tea. GIDA J Food. 2010; 35: 161-68.

Fan M, Chen J. Studies on antimicrobial activity of extracts from thyme. Wei Sheng Wu Xue Bao. 2001; 41: 499-504.

Gnyther BD, Curtis DR. Pyridazinyl GABA derivatives as GABA and glycine antagonist in the spinal cord of the cat. Neurosci Lett. 1986; 68: 585-87.

Ha JH, Lee DU, Lee JT, Kim JS, Yong CS, Kim JA. 4-Hydroxy-benzaldehyde from gastrodiaelata as active in the anti-oxidation and GABAergic neuromodulation of the rat brain. J Ethnopharmacol. 2004; 73: 32933.

Kokate CK, Purohit AP, Gokhale SB. Pathway to screen phytochemical nature of natural drugs. In: Pharmacognosy. 39th ed. India, Nirali Prakashan, 2007, pp 607-11.

Kone WM, Atindehou C, Terreaux K, Hostettmann D, Traore, Dosso M. Traditional medicine in North Cote-d'Ivoire: screening of 50 medicinal plants for antibacterial activity. J. Ethnopharmacol. 2004; 93: 43-49.

Michael, S.A. Life of its own. USA, The New Yorker, 2009, pp 1-50.

Mori A, Nishino C, Enoki N, Tawata S. Antibacterial activity and mode of action of plant flavonoids against Proteus vulgaris and Staphylococcus aureus. Phytochemistry 1987; 26: 2231-34.

Mozaffarian V. Studies on the flora of Iran, four new species and a short note on an interesting Rubiaceae. Ira J Bot. 2006; 107-13.

Nishanta R, Cory S, Harris, Towers GHN. Antimicrobial activity of plants collected from serpentine outcrops in Sri Lanka. Pharm Biol. 2002;.3: 235-44.

Noor AT, Perveen S, Begum A, Fatima I, Malik A, Tareen RB. Rosenones A and B, new anthraquinone derivatives from Aitchisonia rosea. J Asian Nat Prod. 2009a; 4: 209-12.

Noor AT, Perveen S, Begum A, Fatima I, Malik A, Tareen, RB. Aitchisonides A and B, new iridoid glucosides from Aitchisonia rosea. J Asian Nat Prod. 2009b; 11: 985-89.

Olayemi MA, Oluwasola OO. Effect of light irradiation on the antimicrobial activity of Zanthoxylum zanthoxyloides (lam) methanolic extract. Afr J Pharmacol. 2010; 4: 145-50.

Riaz M, Rasool N, Bukhari IH, Shahid M, Zubair M, Rizwan K, Rashid U. In vitro antimicrobial, antioxidant, cytotoxicity and GC-MS analysis of Mazus goodenifolius. Molecules 2012; 17: 14275-87.

Rogawski MA. Revisiting AMPA receptors as an antiepileptic drug target. Epilepsy Curr. 2011; 11: 56-63.

Sarker SD, Nahar L, Kumarasamy, Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 2007; 4: 321-24.

Sharma P, Sharma JD. In vitro hemolysis of human erythrocytes by plant extracts with antiplasmodial activity. J Ethnopharmacol. 2001; 74: 239-43.

Sultana B, Anwar F, Przybylyski R. Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica and Eugenia jambolana Lam. Trees. Food Chem. 2007; 104: 1106-14.

Swinyard EA, Woodhead JH, White HS, Franklin MR. General Principles: Experimental selection, quantification and evaluation of anticonvulsants. In: Antiepileptic drugs. Levy R, Mattson R, Meldrum B, Penry JK, Dreifuss FE (eds). New York, Raven Press, 1989, pp 85-103.

Uddin SJ, Grice ID, Tiralongo E. Cytotoxic effects of Bangladeshi medicinal plant extracts. Evid Based Complement Altern Med. 2011; 1-7.

United States Pharmacopoeia. 28th ed. U.S.A, The United States Pharmacopeial Convention, 1980, pp 864-69.

Vogel HG, Vogel WH. Drug discovery and evaluation, pharmacological assay. Berlin, Springer. 1997; 260-61.

Yuste J, Fung DY. Inactivation of Salmonella typhimurium and Escherichia coli O157: H7 in apple juice by a combination of nisin and cinnamon. J Food Prod. 2004; 67: 371-77.

Published
2015-11-26
How to Cite
Rasool, S., F. Khan, and M. Ahmad. “Evaluation of Anticonvulsant, Antimicrobial and Hemolytic Activity of Aitchisonia Rosea”. Bangladesh Journal of Pharmacology, Vol. 10, no. 4, Nov. 2015, pp. 980-7, doi:10.3329/bjp.v10i4.24153.
Section
Research Articles