Tectorigenin inhibits Caco-2 human colon cells via NF-κB pathway suppression

Authors

  • Xiao-Yu Dai Department of Anus and Intestine Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Yong-Ming Yu Department of Anus and Intestine Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Zheng-Fang Kong Department of Anus and Intestine Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Yi-Sheng Cao Department of Anus and Intestine Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Dan-Dan Huang Department of Stem Cell Laboratory, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Xiao-Rong Hu Department of Stem Cell Laboratory, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Zuo-An Huang Department of Stem Cell Laboratory, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Yang-Yang Xie Department of Anus and Intestine Surgery, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China
  • Shun Zhang Department of Stem Cell Laboratory, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315010, China http://orcid.org/0000-0002-7652-2716

DOI:

https://doi.org/10.3329/bjp.v10i4.23833

Keywords:

Caco-2 human colon cell, NF-κB pathway, Tectorigenin

Abstract

Effective immunomodulator and pro-inflammatory cytokine, Tumor necrosis factor-α (TNF-α) are considered to be responsible for connecting autoimmune pathological process and contagious diseases. TNF-α stimulates the C-X-C motif chemokine 10 (CXCL10) expression that is participated in migration of tumor, metastasis, and invasion. Tectorigenin, an o-methylated isoflavone, is present as a major portion in the Iris tectorum rhizomes. In this study, we investigated the tectorigenin effects as an anticancer drug. The obtained results showed that tectorigenin hinders the invasion of human colon cancer cells (Caco-2). We used reverse transcription PCR, q-PCR and enzyme linked immunosorbent assays to test whether the tectorigenin is involved in the inhibition of TNF-α induced CXCL-10 expression in the Caco-2 cell lines. Further, we tested TNF-α induced NF-κB activity using the tectorigenin. Collective results showed that tectorigenin inhibits the CXCL10 production by using TNF-α via NF-κB inhibition.

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Published

2015-11-18

How to Cite

Dai, X.-Y., Y.-M. Yu, Z.-F. Kong, Y.-S. Cao, D.-D. Huang, X.-R. Hu, Z.-A. Huang, Y.-Y. Xie, and S. Zhang. “Tectorigenin Inhibits Caco-2 Human Colon Cells via NF-κB Pathway Suppression”. Bangladesh Journal of Pharmacology, vol. 10, no. 4, Nov. 2015, pp. 948-55, doi:10.3329/bjp.v10i4.23833.

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Section

Research Articles