Evaluation of in vitro and in vivo anti-arthritic potential of Berberis calliobotrys

Authors

  • . Alamgeer Department of Pharmacology, Faculty of Pharmacy, University of Sargodha, Sargodha http://orcid.org/0000-0003-4775-7337
  • Umme Habiba Hasan Department of Pharmacology, Faculty of Pharmacy, University of Sargodha, Sargodha
  • Ambreen Malik Uttra Department of Pharmacology, Faculty of Pharmacy, University of Sargodha, Sargodha
  • Shahid Rasool Department of Pharmacology, Faculty of Pharmacy, University of Sargodha, Sargodha

DOI:

https://doi.org/10.3329/bjp.v10i4.23779

Keywords:

Anti-arthritic, Berberis calliobotrys, Rheumatoid factor

Abstract

The present study was commenced to evaluate the anti-arthritic effect of 70% methanol extract and n-butanol and aqueous fractions of Berberis calliobotrys using both in vitro and in vivo arthritis models. Extract and fractions were investigated in vitro for inhibition of protein (bovine serum and egg albumin) denaturation and human red blood cell membrane stabilization. In vivo anti-arthritic activity of extract and fractions at 50, 100 and 200 mg/kg was assessed using turpentine oil and formaldehyde-induced arthritis, while, 200 mg/kg dose was evaluated against complete Freunds adjuvant-induced arthritis. B. calliobotrys produced significant (p<0.001) dose dependent inhibition of protein denaturation and human red blood cell membrane stabilization. In turpentine oil, formaldehyde and complete Freunds adjuvant-induced arthritis models, B. calliobotrys significantly (p<0.001) reduced joint and paw swelling. B. calliobotrys markedly improved body weight, hematology profile, radiological and histopathological parameters in complete Freunds adjuvant model. It could be concluded that B. calliobotrys holds anti-arthritic potential, supporting its traditional use in treatment of rheumatoid arthritis.

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Author Biography

. Alamgeer, Department of Pharmacology, Faculty of Pharmacy, University of Sargodha, Sargodha

Assistant Professor

Published

2015-09-30

How to Cite

Alamgeer, ., U. H. Hasan, A. M. Uttra, and S. Rasool. “Evaluation of in Vitro and in Vivo Anti-Arthritic Potential of Berberis Calliobotrys”. Bangladesh Journal of Pharmacology, vol. 10, no. 4, Sept. 2015, pp. 807-19, doi:10.3329/bjp.v10i4.23779.

Issue

Section

Research Articles