Anticancer and apoptotic effects of theaflavin-3-gallate in non-small cell lung carcinoma

Authors

  • Da-Wei Li Department of Cardiothoracic Surgery, Laigang Hospital Affiliated to Taishan Medical College, Laiwu, Shandong 271104
  • Long Meng Department of Thoracic surgery, Shandong Provincial Hospital, Ji?nan, Shandong 250021
  • Kui-Xing Zhang Department of Cardiothoracic Surgery, Laigang Hospital Affiliated to Taishan Medical College, Laiwu, Shandong 271104
  • Wei-Ke Zhang Department of Oncology, Laigang Hospital Affiliated to Taishan Medical Colledge, Laiwu, Shandong 271104 http://orcid.org/0000-0002-0572-7546

DOI:

https://doi.org/10.3329/bjp.v10i4.23580

Keywords:

Cell cycle, Cell migration, Lung cancer, Theaflavin-3-gallate

Abstract

The objective was to determine the antiproliferative and apoptotic effects of theaflavin-3-gallate in human non-small cell lung cancer cells (A-549) along with determining the effect on cell cycle phase distribution, cell migration and invasion. Cell viability was determined by MTT assay while as phase contrast and fluorescence microscopies were involved to study apoptotic morphologi-cal features in these cells. Flow cytometry investigated the effect of theaflavin-3-gallate on cell cycle phase distribution. Theaflavin-3-gallate treatment led to a substantial cytotoxic effect in A-549 cancer cells with IC50 values of 42.1 µM and 27.9 µM at 24 and 48 hours respectively. Further, 80 and 160 µM dose of theaflavin-3-gallate induced apoptotic features including chromatin margina-tion and micronuclei presence. The population of cells in G2/M phase increased from 2.7% (control) to 6.8%, 17.2% and finally to 46.5% after treatment with 20, 80 and 160 µM concentration of theaflavin-3-gallate respectively indicating G2/M phase cell cycle arrest.

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Published

2015-09-27

How to Cite

Li, D.-W., L. Meng, K.-X. Zhang, and W.-K. Zhang. “Anticancer and Apoptotic Effects of Theaflavin-3-Gallate in Non-Small Cell Lung Carcinoma”. Bangladesh Journal of Pharmacology, vol. 10, no. 4, Sept. 2015, pp. 790-8, doi:10.3329/bjp.v10i4.23580.

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Section

Research Articles