Alantolactone pyrazoline analogue inhibits cancer cell proliferation and induces apoptosis in non-small cell lung carcinoma cells

Authors

  • Jing lv Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao 266000
  • Ming-Qin Cao Health Examination Center, Dongying People?s Hospital, Dongying 257091
  • Jian-Chun Yu Department of Emergency, Linzi District People?s Hospital, Zibo 255400

DOI:

https://doi.org/10.3329/bjp.v10i2.22619

Keywords:

Alantolactone pyrazoline analogue, Anti-cancer, Apoptosis, Cell cycle, Lung cancer

Abstract

The aim of the current study was to evaluate the anticancer and apoptotic effects of alantolactone pyrazoline analogue in human non-small cell lung cancer (NCI-H460) cells. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide) assay was used to evaluate the cell viability while as fluorescence microscopy was used to assess the effect on apoptosis, cellular and nuclear morphology. Flow cytometry evaluated the effect of APA on cell cycle arrest in these cells. The results revealed that APA induced potent, time and dose-dependent cytotoxic effects on the growth of NCI-H460 cells. It also inhibited colony forming tendency as well as cell invasion capability of these cancer cells. APA induced dose-dependent nuclear and cellular morphological effects including chromatin condensation and DNA fragmentation. Flow cytometry revealed that the anticancer effects of APA might be due to its cell cycle arrest inducing tendency in G0/G1 phase of the cell cycle.

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Published

2015-05-05

How to Cite

lv, J., M.-Q. Cao, and J.-C. Yu. “Alantolactone Pyrazoline Analogue Inhibits Cancer Cell Proliferation and Induces Apoptosis in Non-Small Cell Lung Carcinoma Cells”. Bangladesh Journal of Pharmacology, vol. 10, no. 2, May 2015, pp. 409-15, doi:10.3329/bjp.v10i2.22619.

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Section

Research Articles