Antihyperlipidemic effect of Berberis orthobotrys in hyperlipidemic animal models

  • . Alamgeer Faculty of Pharmacy University of Sargodha, Sargodha
  • Aqsa Ghuffar Faculty of Pharmacy University of Sargodha, Sargodha
  • Taseer Ahmad Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Islamabad
  • Muhammad Naveed Mushtaq Faculty of Pharmacy University of Sargodha, Sargodha
Keywords: Berberis orthobotrys extract, antihyperlipidemic, atheroprotective, Sprague Dawley rats


The present study was undertaken to evaluate antihyperlipidemic effect of aqueous methanolic extract of Berberis orthobotrys in experimentally induced hyperlipidemic animal models. Atorvastatin was used as standard drug. The crude extract (50 and 100 mg/kg) significantly prevented the increase in low density lipoprotein (LDL), very low density lipoprotein (VLDL), total cholesterol (TC), triglyceride (TG), atherogenic index and coronary risk index in high fat diet, cholesterol, fructose and olive oil-induced hyperlipidemic rat models whereas in ethanol treated mice, the extract reduced VLDL, TG and TC levels. The high density lipoprotein (HDL) level was significantly increased in high fat diet, olive oil and cholesterol induced hyperlipidemic rats. The reduction in serum level of above parameters with 100 mg/kg dose of extract was comparable with atorvastatin. It is concluded that B. orthobotrys has atheroprotective and lipid lowering potential.


Download data is not yet available.
1017 Read

Author Biography

. Alamgeer, Faculty of Pharmacy University of Sargodha, Sargodha


Abbott R, Wilson P, Kannel W, Castelli WP. High density lipoprotein-cholesterol, total cholesterol screening and myocardial infarction. The Framingham Study. J Am Heart Assoc. 1988; 8: 207-11.

Alamgeer, Malik MNH, Bashir S, Khan AQ, Mushtaq MN, Rashid M, Akram M, Samreen.S. Evaluation of diuretic activity of Paspalidium flavidum in rats. Bangladesh J Pharmacol. 2013; 8: 177-80.

Alladi S, Khada S. Induction of hypercholesterolemia by supplementing soy protein with acetate generating amino acids. Nutr Rep Int. 1989; 40: 893-99.

Akerele O, Heywood V, Synge H. The conservation of medicinal plants. United Kingdom, Cambridge University Press, 1991.

Byron J, Dennis L, Vijay N. A truly deadly quartet: obesity, hypertension, hypertriglyceridemia, and hyperinsulinemia. Cleveland Clinic J Med. 2002; 69: 989-89.

Carr MC, Brunzell JD. Abdominal obesity and dyslipidemia in the metabolic syndrome: importance of type 2 diabetes and familial combined hyperlipidemia in coronary artery disease risk. J Clin Endocrinol Metab. 2004; 89: 2601-07.

Chapman M, Assmann G, Fruchart J, Shephard J, Sirtori C. Raising high-density lipoprotein cholesterol with reduction of cardiovascular risk: the role of nicotinic acid: A position paper developed by the European Consensus Panel on HDL-C. J Curr Med Res Opin. 2004; 20: 1253-68.

Emily S. Van A, Mieke D, Maud N, Jessica R, Jhon J. Clinical diagnostic and therapeutic aspects of (inherited) hypercholesterolemia. J Drug Discov Today Dis Mech. 2004; 1: 165-70.

Ferreira JM, Sousa DF, Dantas MB, Fonseca SGC, Menezes DB, Martins AMC, deQueiroz MGR. Effects of Bixa orellana L. seeds on hyperlipidemia. Phytother Res. 2013; 27: 14447.

Friedewald V, Levy R, Fredrickson S, Estimation of low density lipoprotein cholesterol in plasma, without use of the preparative centrifuge. Clin chem. 1972; 18: 499-504.

Goodman and Gilmans The pharmacological basis of therapeutics. 10th ed. Hardman J.G., Limbird L.E. (eds.). New York, The McGraw Hill Co. Inc., 2001.

Guo H, Ling W, Wang Q, Liu C, Hu Y, Xia M, et al. Effect of anthocyanin-rich extract from black rice (Oryza sativa L. indica) on hyperlipidemia and insulin resistance in fructose-fed rats. J Plant Foods Human Nutr. 2007; 62: 1-6.

Jain K, Kathiravan M, Somani R, Shishoo CJ. The biology and chemistry of hyperlipidemia. J Bioorg Med Chem. 2007; 15: 4674-99.

Khanna A, Kumar V, Mubin M, Ranjana S, Sushma S, Remesh C, Farzana M, Ali M, Jitendra K, Raj. Lipid lowering activity of Anthocephalus indicus root in hyperlipidemic rats. J Evid Based Complement Alternat Med. 2008; 7: 31722.

Leiber S, Schmid R. The effect of ethanol on fatty acid metabolism: stimulation of hepatic fatty acid synthesis in vitro. J Clin Invest. 1961; 40:39499.

Mokdad A, Ford E, Bowman B, Dietz W, Vinicor F, Bales V, Marks J. Prevalence of obesity, diabetes and obesity related health risk factors. J Am Med Assoc. 2003; 289:76-79.

Panchamoorthy R, Mambakkam K, Ravichandran C. Venkatraman A. Intraperitoneal l-carnitine regulates lipid metabolism and reduces oxidative stress in fructose-induced hyperlipidemic rats. J Diab Croa. 2005; 34: 89-95.

Santosh Kumar J, Manjunath S, Sakhare Pranavkumar M. A study of anti-hyperlipidemia, hypolipedimic and anti-atherogenic activity of fruit of Emblica officinalis (amla) in high fat fed albino rats. Int J Med Res Health Sci. 2012; 2: 70-77.

Schwartz C, Valente A, Sprague E. A modern view of atherogenesis. Am J Cardiol. 1993; 71: 9b14b.

Silwa R, santos F, Rao V, Macial M, Pinto A. Blood glucose and triglyceride lowering effect of trans-dehydrocrotonin a diterpene from croton cajucara benth, in rats. J Diab Obes Meteb. 2001; 3: 452-56.

Spence JT, Koudelka AP, Tseng-Crank JC. Role of protein synthesis in the carbohydrate-induced changes in the activities of acetyl-CoA carboxylase and hydroxymethylglutaryl-CoA reductase in cultured rat hepatocytes. Biochem J. 1985; 227: 939-47.

Srikanth I, Vikas K. Anti-hyperlipidemic activity of hyperforin: An in vivo study in rats. Int J Pharmacol Online. 2009; 2: 133-39.

Zulet A, Barber A, Garcin H, Higueret P, Alfredo M. Alterations in carbohydrate and lipid metabolism induced by diet rich in coconut oil and cholesterol in a rat model. J Am Col Nutr. 1999; 18: 36-42.

How to Cite
Alamgeer, ., A. Ghuffar, T. Ahmad, and M. Mushtaq. “Antihyperlipidemic Effect of Berberis Orthobotrys in Hyperlipidemic Animal Models”. Bangladesh Journal of Pharmacology, Vol. 9, no. 3, Aug. 2014, pp. 377-82, doi:10.3329/bjp.v9i3.19922.
Research Articles