Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling

  • Yang Chen Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003
  • Xiang-Zhen Han Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003
  • Wei Wang Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003
  • Ren-Tao Zhao Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003
  • Xiao Li Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003
Keywords: Withaferin A, Withanolides, Adenocarcinoma, Phosphorylation, Apoptosis


Human osteosarcoma cells were treated with withaferin A and the antiproliferative activity was evaluated by the 3-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Real-time RT-PCR and western blot were used to investigate whether the down-regulation of Notch-1 contributes to withaferin A-induced inhibition of proliferation in osteosar-coma cells. The results showed that withaferin A caused marked inhibition of osteosarcoma cell growth and G2?M phase cell cycle arrest. This was associated with concomitant attenuation of Notch-1 and down-regulation of its downstream genes, such as matrix metalloproteinases. These results suggest that antitumor activity of withaferin A is mediated through a novel mechanism involving inactivation of the Notch-1 signalling pathway. Our data provide the first evidence that the down regulation of Notch-1 by withaferin A may be an effective approach for the treatment of osteosarcoma.


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Author Biography

Xiao Li, Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003
Emergency Department of Bone Tumor


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How to Cite
Chen, Y., X.-Z. Han, W. Wang, R.-T. Zhao, and X. Li. “Withaferin A Inhibits Osteosarcoma Cells through Inactivation of Notch-1 Signalling”. Bangladesh Journal of Pharmacology, Vol. 9, no. 3, Aug. 2014, pp. 364-70, doi:10.3329/bjp.v9i3.19568.
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