Effects of aqueous extract of Fadogia agrestis stem in alloxan-induced diabetic rats

  • Musa T. Yakubu Phytomedicine, Toxicology, Reproductive and Developmental Biochemistry Research Laboratory, Department of Biochemistry, University of Ilorin
  • Olalekan B. Ogunro Phytomedicine, Toxicology, Reproductive and Developmental Biochemistry Research Laboratory, Department of Biochemistry, University of Ilorin
Keywords: Alloxan, Diabetes, Fadogia agrestis, Glibenclamide

Abstract

Aqueous extract of Fadogia agrestis stem at the doses of 18, 36, and 72 mg/kg body weight was investigated for antidiabetic activity in alloxan-induced diabetic rats. The results revealed that the administration of alloxan significantly increased (p<0.05) the levels of blood and hepatic glucose, serum urea, creatinine, albumin, total cholesterol, triacylglycerol, low-density lipoprotein cholesterol, atherogenic index and very low-density lipoprotein cholesterol whereas the organ/body weight ratio, activity of hexokinase, levels of high-density lipoprotein cholesterol, packed cell volume and hemoglobin reduced significantly. In contrast, all the doses of the extract significantly reversed the levels of these biomolecules/biochemical parameters. These reversals, however, did not compare well with their respective control values by the end of exposure period. Overall, the aqueous extract of F. agrestis stem showed promise as an antidiabetic agent.

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References

Abdel-Moneim A, El-Feki M, Salh E. Effect of Nigella sativa, fish and glicazide on alloxan diabetic rats. 1- Biochemical and histopathological studies. J Egyptian German Soc. 1997; 23: 237-65.

Alamgeer, Rashid M, Bashir S, Mushtaq MN, Khan HU, Malik MNH, Qayyum A, Muhammad Rahaman MSU. Comparative hypoglycemic activity of different extracts of Teucrium stocksianum in diabetic rabbits. Bangladesh J Pharmacol. 2013; 8: 186-93.

Aly F, Fatania H, Shamte U. The effect of a plants mixture extract on liver gluconeogenesis in streptozotocin induced diabetic rats. Diabetes Res Clin Exp. 2010; 18: 163-68.

Ameen OM, Olatunji G, Atata RF, Usman LA. Antimicrobial activity, cytotoxic test and phytochemical screening of extracts of the stem of Fadogia agrestis. Nigerian Soc Exptal Biol J. 2011; 11: 79-84.

Ananda PK, Kumarappan CT, Sunil C, Kalaichelvan VK. Effect of Biophytum sensitivum on streptozotocin and nicotinamide-induced diabetic rats. Asian Pac J Tropical Biomed. 2012, 11: 31-35.

Anero RI, IXaz-Lanza E, Ollivier B, Baghdikian G, Balansard MB. Monoterpene glycosides isolated from Fadogia agrestis. Phytochem. 2008; 69: 805-11.

Aruna RV, Ramesh B, Kartha VN. Effect of beta-carotene on protein glycosylation in alloxan induced diabetic rats. Indian J Exp Biol. 1999; 37: 399-401.

Barham D, Trinder P. An improved colour reagent for the determination of blood glucose by oxidase system. Analyst. 1972; 97: 142-45.

Brandstrup N, Kirk JE, Bruni C. Determination of hexokinase in tissues. J Gerontol. 1957; 12: 166-71.

Dey L, Xie JT, Wang A, Wu J, Maleckar SA, Yuan CS. Antihyperglycemic effects of ginseng: comparison between root and berry. Phytomed. 2003; 10: 600-05.

Fredrickson DS, Levy RI, Lees RS. Fat transport in lipoproteins: An integrated approach to mechanisms and disorders. New Engl J Med. 1967; 276: 148-56.

Friedewald WT, Levy RI, Fedrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18: 499-502.

Goodman LS, Gilman A. The pharmacological basis of therapeutics. 7th ed. New York, MacMillan, 1985, pp 1490-510.

Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol. 2002; 81: 81-100.

Gupta R, Gabrielsen B, Ferguson FM. Natures Medicines: Traditional knowledge and intellectual property management. case studies from the National Institutes of Health (NIH). USA. Curr Drug Discov Technol, 2005; 2: 203-19.

Hainline AJR, Cooper GR, Olansky AS. CDC Survey of high density lipoprotein cholesterol measurement: A report. Atlanta Lipid Standardization Program. Center for Disease Control. 1980, p 3.

Kemp A, Kits Van Heijningen AJM. A colorimeter micro-method for the determination of glycogen in tissues. Biochem J. 1954; 6: 646-48.

Khushbaktova KM, Ozsoy-Sacan O, Bolkent S, Yanardag R. Effect of glurenorm on immunohistochemical changes in pancreatic beta-cells of rats in experimental diabetes. Indian J Exp Biol. 2005; 43: 268-71.

Kumar GPS, Arulselvan P, Kumar DS, Subramanian SP. Antidiabetic activity of fruits of Terminalia chebula on streptozotocin-induced diabetic rats. J Health Sci. 2006; 52: 283-91

Mansi KMS. Effects of administration of alpha-melanocyte stimulating hormone (?-MSH) on some hematological values of alloxan-induced diabetic rats. Am J Pharmacol Toxicol. 2006; 1: 5-10.

Mooradian AD. Dyslipidemia in type 2 diabetes mellitus. Nature Clin Practice Endocrinol Metab. 2009; 5: 150-59.

Moss PP. Blood banking: Concepts and applications. Philadelphia, W.B. Saunders Co., 1999, pp 12-34.

Muhammad NO, Oloyede OB. Haematological parameters of broiler chicks fed Aspergillus niger - fermented Terminalia catappa seed meal-based diet. Global J Biotechnol Biochem. 2009; 4: 179-83.

Nagappa AN, Thakurdesai PA, Venkat RN, Singh J. Antidiabetic activity of Terminalia catappa Linn fruits. J Ethnopharmacol. 2003; 88: 45-50.

Ng TKW, The CB, Vidyadaran MK, Tee ES, Thong ML, Kandiah M, Ehalid AH. A critical evaluation of high density lipoprotein cholesterol as an index of coronary artery disease risk in Malaysians. Malaysian J Nutr. 1997; 3: 61-70.

Noor A, Gunasekaran S, Manickam AS, Vijayalakshmi MA. Antidiabetic activity of Aloe vera and histology of organs in streptozotocin-induced diabetic rats. Curr Sci. 2008; 94: 1070-76.

Oyedemi SO, Yakubu MT, Afolayan AJ. Antidiabetic activities of aqueous leaves extract of Leonotis leonurus in streptozotocin induced diabetic rats. J Med Plants Res. 2011; 5: 119-25.

Pari L, Rajarajeswari N. Efficacy of coumarin on hepatic key enzymes of glucose metabolism in chemical induced type 2 diabetic rats. Chemico-Biological Interaction. 2009; 181: 292-96.

Pattanayak S, Nayak SS, Panda D, Shende V. Hypoglycemic of Cajanus scarabaeoides in glucose overloaded and streptozotocin-induced diabetic rats. Bangladesh J Pharmacol 2009; 4: 131-35.

Saha BK, Bhuiyan NH, Mazumder K, Haque KMF. Hypogly-cemic activity of Lagerstroemia speciosa L. extract on streptozotocin-induced diabetic rat: Underlying mechanism of action. Bangladesh J Pharmacol 2009; 4: 79-83.

Sanon S, Olliver E, Azas N, Mahiou V, Gassquet M, Quattara CT, Nebie I, Traore AS, Esposito F, Balansard G, Timon-David P, Fumoux F. Ethnobotanical survey and in vitro antiplasmodial activity of plant used in traditional medicine in Burkina Faso. J Ethnopharmacol. 2003; 86: 143-47.

Sarabu R, Tilley J. Recent advances in therapeutic approaches to type 2 diabetes. In: Annual reports in medicinal chemistry. Doherty AM (ed). Elsevier, Academic Press, 2005; 40: 167-81.

Shalm OW, Jain NC, Carroll EJ. Veterinary haematology. 3rd ed. Philadelphia, Lea and Fabiger, 1995, pp 498-512.

Sharma N, Garg V, Paul A. Antihyperglycemic, antihyperlipidemic and antioxidative potential of Prosopis cineraria bark. Indian J Clin Biochem. 2010; 25: 193-200.

Sheela CG, Augusti KT. Antidiabetic effects of S-allyl cysteine sulphoxide isolated from garlic Allium sativum Linn. Indian J Exp Biol. 1992; 30: 523-26.

Szkudelski T. The mechanism of alloxan and streptozotocin action on ?-cells of the rat pancreas. Physiol Res. 2001; 50: 536-46.

Temme E, Van Hoydonck PG, Schouten EG, Kesteloot H. Effect of plant sterol-enriched spread on serum lipids and lipoproteins in mildly hypercholesterolaemic subjects. Acta Cardiol. 2002; 57: 111-15.

Tietz NW. Clinical guide to laboratory tests. 3rd ed. Philadelphia, W. B. Saunders Co., 1995.

Wadkar KA, Magdun CS, Patil SS, Naikwade NS. Antidiabetic potential and Indian medicinal plants. J Herbal Med Toxicol. 2008; 2: 45-50.

Yakubu MT, Akanji MA, Oladiji AT. Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Heirn) stem in male albino rats. Asian J Androl. 2005; 7: 399-404.

Yakubu MT, Akanji MA, Oladiji AT. Alterations in serum lipid profile of male rats by oral administration of aqueous extract of Fadogia agrestis stem. Res J Med Plant. 2008; 2: 66-73.

Yakubu MT, Akanji MA, Nafiu MO. Antidiabetic activity of aqueous of Cochlospermum planchonii root in alloxan-induced diabetic. Cameroon J Exp Biol. 2010; 6: 91-100.

Yao XH, Chen L, Nyomba BL. Adult rats prenatally exposed to ethanol have increased gluconeogenesis and impaired insulin response of hepatic gluconeogenic genes. J Appl Physiol. 2006; 100: 642-48.

Published
2014-08-13
How to Cite
Yakubu, M., and O. Ogunro. “Effects of Aqueous Extract of Fadogia Agrestis Stem in Alloxan-Induced Diabetic Rats”. Bangladesh Journal of Pharmacology, Vol. 9, no. 3, Aug. 2014, pp. 356-63, doi:10.3329/bjp.v9i3.18610.
Section
Research Articles