Screening of antimicrobial compound from the sea slug Armina babai

  • Mayavan Sivaprakasam Ramya Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
  • Kanagasabapathy Sivasubramanian Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
  • Samuthirapandian Ravichandran Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
  • Ramasamy Anbuchezhian Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
Keywords: Antibacterial, Armina babai, SDS-PAGE, Sea slug

Abstract

Different solvents such as acetone, butanol, ethanol, hexane and methanol of Sea slug Armina babai was evaluated for its biomedical properties. Most potent extracts were purified using silica gel column and the active fractions were characterized by TLC, SDS-PAGE and FTIR. Maximum zone of inhibition was recorded against Pseudomonas sp., and minimum zone of inhibition was recorded against Proteus mirabilis by butanol extracts. TLC profiling with Rf value 0.82 indicating the presence of amino acids and peptides. Total protein was estimated as 21.36% with molecular weight range between 13 and 72 kDa in SDS-PAGE. The FT-IR spectrum of fractions, obtained from sea slug, reveals characteristic functional groups range between 465.75 and 3423.82 cm-1. Thus the obtained results indicate the presence of potent antimicrobial compounds in sea slug A. babai may pave the way to explore the potential development of new compounds to be launched in the pharmaceutical filling.

Downloads

Download data is not yet available.
Abstract
1547
Download
921 Read
228

References

Abu GO, Weiner RM, Rice J, Colwell RR. Properties of an extracellular adhesive polymer from the marine bacterium Shewanella colwelliana. Bio foul. 1991; 3: 69-84.

Anderson RS, Beaven AE. Antibacterial activities of oyster (Crassostrea virginica) mussel (Mytilus edulis and Geukensia demissa) plasma. Aquat Living Resour. 2001; 14: 343-49.

Bagińska K, Makowska J, Wiczk W, Kasprzykowski F, Chmurzyński L. Conformational studies of alanine-rich peptide using CD and FTIR spectroscopy. J Peptide Sci. 2008; 14: 283–89.

Benkendorff K, Bremner JB, Davis AR. Indole derivatives from the egg masses of Muricid molluscs. Molecules 2001; 6: 70-78.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dyebinding. Anal Bio chem. 1976; 72: 248-54.

Cimino G, Fontana A, Cutignano A, Gavagnin M. Biosynthesis in opisthobranch molluscs: General outline in the light of recent use of stable isotopes. Phytochem Rev. 2004; 3: 285-307.

Johnson PM, Willows AOD. Defense in sea hares (Gastropoda, Opisthobranchia, Anaspidea): Multiple layers of protection from egg to adult. Mar Fresh Behav Physiol. 1999; 32: 147–80.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-85.

Ngameni B, Kuete V, Simo IK, Mbaveng AT, Awoussong PK, Patnam R, et al. Antibacterial and antifungal activities of the crude extract and compounds from Dorstenia turbinata (Moraceae). S Afr J Bot. 2009; 75: 256-61.

Patterson Edward JK, Murugan A. Screening of cephalopods for bioactivity. Phuket. Marine Biological Center Space Weather Prediction Center Publications, 2000; 21: 253-56.

Prem Anand T, Patterson Edward JK. Antimicrobial activity in the tissue extracts of five species of cowries Cyprea sp. (Mollusca: Gastropoda) and an ascidian Didemnum psammathodes (Tunicata: Didemnidae). Indian J Mar Sci. 2002; 25: 239-08.

Ramasamy P, Subhapradha N, Srinivasan A, Shanmugam V, Jayalakshmi K, Shanmugam A. In vitro evaluation of antimicrobial activity of methanolic extract from selected species of Cephalopods on clinical isolates. Afr J Microbiol Res. 2011a; 5: 3884-89.

Ramasamy P, Vino AB, Saravanan R, Subhapradha N, Shanmugam V, Shanmugam A. Screening of antimicrobial potential of polysaccharide from cuttlebone and methanolic extract from body tissue of Sepia prashadi Winkworth, 1936. Asian Pac J Trop Biomed. 2011b; 1: S244-48.

Rodrigues E, Tilvi S, Naik CG. Antimicrobial activity of marine organisms collected off the coast of South East India. J Exp Mar Biol Ecol. 2004; 309: 121-27.

Shanmugam A, Amalraj T, Palpandi C. Antimicrobial activity of sulfated mucopolysaccharides [heparin and heparin–like glycosaminoglycans (GAGs)] from cuttlefish Euprymna berryi, Sasaki, 1929. Trends Applied Sci Res. 2008; 3: 97-102.

Vennila R, Kumar RK, Kanchana S, Arumugam M, Balasubramanian T. Investigation of antimicrobial and plasma coagulation property of some molluscan ink extracts: Gastropods and cephalopods. Afr J Biochem Res. 2011; 5: 14-21.

Voultsiadou E. Therapeutic properties and uses of marine invertebrates in the ancient Greek world and early Byzantium. J Ethnopharmacol. 2010; 130: 237-47.

Wägele H, Ballesteros M, Avila C. Defensive glandular structures in opisthobranch mollusks-from histology to ecology: In Gibson RN, Atkinson RJA, Gordon JDM, editors. Oceanography and Marine Biology: An Annual Review CRC Press; 2006; 44: 197-276.

Wägele H, Klussmann-Kolb A. Opisthobranchia (Mollusca, Gastropoda): More than just slimy Slug, shell reduction and it implications on defence and foraging. Front Zool. 2005; 2: 1-18.

Published
2014-07-03
How to Cite
Ramya, M., K. Sivasubramanian, S. Ravichandran, and R. Anbuchezhian. “Screening of Antimicrobial Compound from the Sea Slug Armina Babai”. Bangladesh Journal of Pharmacology, Vol. 9, no. 3, July 2014, pp. 268-74, doi:10.3329/bjp.v9i3.18483.
Section
Research Articles