Hepatoprotective activity of Chenopodium murale in carbon tetrachloride-induced hepatic damage in rabbits

  • Aftab Ullah Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur
  • Mahmood Ahmad Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur
Keywords: Chenopodium murale, Hepatoprotective activity, CCl4, Silymarin, serum enzymes, Histopathology

Abstract

The main purpose of this study was to determine the in vivo hepatoprotective activity of the ethanol extract of whole plant extract of Chenopodium murale (500 and 750 mg/kg; orally) in carbon tetrachloride-induced (0.75 mL/kg; subcutaneously) hepatotoxic rabbit. Silymarin (100 mg/kg/day orally) was used as a standard drug. Hepatotoxic rabbits boosted levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase and total bilirubin. Extracts of C. murale (both doses) proved to have hepatoprotective activity by reducing the elevated level of enzymes. Extract at a dose of 500 mg/kg revealed highly significant (p<0.001) results as compared to 750 mg/kg. Histopathological study of liver tissues additionally authenticated these findings. On the basis of our findings it is concluded that extracts of C. murale can be used in liver disorders.

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References

Ahmad B, Jan Q, Choudhary MI, Nisar M. Phytochemical evaluation of Chenopodium murale L. Asian J Plant Sci. 2003; 2: 1072–78.

Ahmad M, Eram S. Hepatoprotective studies on Haloxylon salicornicum: A plant from cholistan desert. Pakistan J Pharm Sci. 2011; 24: 377-82.

Amaca M, Bilgin HM, Obay BD, Diken H, Kelle M, Kale E. The hepatoprotective effect of coumarin and coumarin derivates on carbon tetrachloride-induced hepatic injury by antioxidant activities in rats. J Physiol Biochem. 2011; 67: 569-76.

Brattin WJ, Pencil SD, Waller RL, Glende EA, Reckenjel O. Assessment of role of calcium ion in hydrocarbon hepatotoxicity. Environ Health Perspect. 1984; 57: 321-23.

Brent JA, Rumack BH. Role of free radicals in toxic hepatic injury. II. Are free radicals the cause of toxin-induced liver injury? J Toxicol Clin Toxicol. 1993; 31: 173-96.

Bhayee A, Sarkar A, Chatterjee M. Hepatoprotective activity of carrot (Dacuscarota Linn) against CCl4-intoxication in mouse liver. J Ethnopharmacol. 1995; 47: 69-74.

Chattopadhyay, RR. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract. Part II. J Ethnopharmacol. 2003; 89: 217-19.

Chatterjee TK. Medicinal plants with hepatoprotective properties in herbal opinions. vol. III. Calcutta, Books and Allied (P) Ltd., 2000, p 135.

Dianzani, MU, Muzia G, Biocca ME, Canuto RA. Lipid peroxidation in fatty liver induced by caffeine in rats. Int J Tissue React. 1991; 13: 79-85.

Elkreemi A, Eideh RA, Zaiter A. The growth of Chenopodium murale irrigated with polluted and unpolluted water: A modeling approach. Australian J Basic Appl Sci. 2009; 3: 1827-37.

Halliwel B, Gutteridge JM. Role of free radicals and catalytic metal ions in human disease: An overview. Methods in enzymology. 1990; 186: 1-85.

Handa SS, Sharma A. Hepatoprotective activity of androgra-pholide from Andrographis paniculata against carbon tetrachloride. Indian J Med Res. 1990; 92: 276-83.

He G, Aoyama Y. Effects of adding some dietary fibers to a cystine diet on the activities of liver antioxidant enzymes and serum enzymes in rats. Biosci Biotechnol Biochem. 2005; 67: 617-21.

Humason GL. Animal tissue techniques. 4th ed. San Francisco, WH Freeman and Company, 1979, pp 1-16.

Johnston DE, Korening C. Mechanism of early carbon tetrachloride toxicity in cultured rat hepatocytes. Pharmacol Toxicol. 1998; 83: 231-39.

Jan G, Khan MA, Jan F. Medicinal value of the Asteraceae of DirKohistan Valley, NWFP. Ethnobotanical Leaflets. 2009; 13: 1205.

Khan M, Farrukh H, Shahana M. Preliminary floristic range of Tehsil Takht-e-Nasrati Pakistan. Int J Biosci. 2011; 23: 88-99.

Khurro AA, Dar GH, Khan ZS, Malik AH. Exploring an inherent interface between taxonomy and biodiversity: Current problems and future challenges. J Nat Conserv. 2007; 15: 256-61.

Liechtfield CJT, Wilcoxon F. A simplified method of evaluating dose-effect experiments. J Pharmacol Exp Ther. 1949; 96: 99-113.

Marie CN. In: Gardens of Hawaii. Bishop Museum Press, 1965, p 331.

Muhammad Z, Wazir SM, Farooq A, Hussain Z. Distribution and checklist of weeds in maize crop of frontier region Bannu, Khyber Patunkhwa, Pakistan. Pakistan J Weed Sci. 2011; 17: 373-79.

Mukherjee PK. Quality control of herbal drugs, 1st ed. New Delhi, Business Horizons Pharmaceutical Publication, 2002, p 531.

Nkosi CZ, Opoku AR, Terblanche SE. Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4-induced liver injury in low protein fed rats. Phy ther Res. 2005; 19: 341–45.

Recknagel RO. A new direction in the study of carbon tetrachloride hepatotoxicity. Life Sci. 1983; 33: 401-08.

Shahani S. Evaluation of hepatoprotective efficacy of APCL: A polyherbal formulation in vivo in rats. Indian Drugs. 1999; 36: 628-31.

Subramoniam A, Pushpangadan P. Development of phytomedicine for liver diseases. Indian J Pharmacol. 1999; 31: 166-75.

Saraf S, Dixit VK, Patnaik GK, Tripathi SC. Antihepatotoxic activity of Euphorbia antisyphilitica. Indian J Pharm Sci. 1996; 58: 137-41.

Shirwaiker A, Sreenivasan KK, Krishnanand BR, Vasanth Kumar A. Chemical investigation and antihepatotixic activity of the root bark of Capparis spinosa. Fitoterapia 1996; 67: 200-04.

Singh T, Sharma A, Sharma LR, Amandeep S. Common weeds of rabi (winter) crops of tehsil Nowshera, District Rajouri (Jammu & Kashmir), India. Pakistan J Weed Sci. 2010; 16: 39-45.

Sturgill MG, Lambert GH. Xenobiotic-induced hepatotoxicity: Mechanisms of liver injury and methods of monitoring hepatic function. Clin Chem. 1997; 43: 1512-26.

Shah M, Patel P, Phadke M, Menon S, Sane RT. Evaluation of the effect of aqueous extract from powders of root, stem, leaves and whole plant of Phyllanthus debilis against CCL4 induced rat liver dysfunction. Indian Drugs. 2002; 39: 333-37.

Sallie R, Tredger JM, William R. Drugs and the liver. Biopharm Drug Dispos. 1999; 12: 251-59.

Tran QL, Adnyana IK, Tezuka Y, Nagaoka T, Tran QK, Kadota S. Triterpene saponins from Vietnamese ginseng (Panax vietnamensis) and their hepatocytoprotective activity. J Nat Prod. 2001; 64: 456-61.

Visioli F, Keaney JF, Halliwell B. Antioxidant and cardiovascular disease; panaceas or tonics for tired sheep? Cardivasc Res. 2000; 47: 409.

Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model. Crit Rev Toxicol. 2003; 33: 105-36.

Woodson RR. Statistical methods for the analysis of biochemical data, series in probability and mathematical statistics, New York, Wiley, 1987, p 316.

Wright JW. The effects of a heart puncture blood sample upon body water maintenance in rats. Physiol Behavior. 1973; 10: 407-10.

Wendel A, Feurensteins S, Konz KH. Acute paracetamol intoxication of starved mice leads to lipid peroxidation in vivo. Biochem Pharmacol. 1987; 28: 2051-53.

Published
2014-03-10
How to Cite
Ullah, A., and M. Ahmad. “Hepatoprotective Activity of Chenopodium Murale in Carbon Tetrachloride-Induced Hepatic Damage in Rabbits”. Bangladesh Journal of Pharmacology, Vol. 9, no. 1, Mar. 2014, pp. 118-23, doi:10.3329/bjp.v9i1.17754.
Section
Research Articles