In vitro study on the hypoglycemic potential of Nicotiana tabacum leaf extracts


  • Mutiu Idowu Kazeem Department of Biochemistry, Lagos State University, P. M. B 0001, Ojo, Lagos
  • Semawon Mauton Ogungbe Department of Biochemistry, Lagos State University, PMB 0001, Ojo, Lagos
  • Gbemisola Morounke Saibu Department of Biochemistry, Lagos State University, PMB 0001, Ojo, Lagos
  • Oluwaseyi Mayode Aboyade South African Herbal Science and Medicine Institute, University of the Western Cape, Bellville 7535, South Africa



Diabetes, Enzyme inhibition, Hyperglycemia, Nicotiana tabacum, Phytochemical


Inhibition of some carbohydrate metabolizing enzymes is one of the modes of action of antihyperglycemic agents. The aim of this study was to investigate the in vitro inhibition of α-amylase and α-glucosidase by extracts of N. tabacum leaf. Powdered leaves were extracted with acetone, ethanol and water, and tested for their ability to inhibit α-amylase from Aspergilus oryzae and α-glucosidase from Saccharomyces cerevisae. The results revealed that aqueous extract of the plant was most effective inhibitor of α-amylase (IC50 5.70 mg/mL) while acetone extract exhibited the best inhibitory potential on α-glucosidase (IC50 4.50 mg/mL). Kinetic studies showed that the mode of inhibition of α-amylase by aqueous extract was non-competitive while that of the acetone extract on α-glucosidase was competitive. The observed inhibitions of α-amylase and α-glucosidase suggest that the leaf extracts of N. tabacum may be useful in the management of Diabetes mellitus, which may due to the presence of phytochemicals.


Download data is not yet available.
2001 Read

Author Biography

Mutiu Idowu Kazeem, Department of Biochemistry, Lagos State University, P. M. B 0001, Ojo, Lagos



Ali H, Houghton PJ, Soumyanath A. Alpha-amylase inhibitory activity of some Malaysian plants used to treat diabetes with particular reference to Phyllanthus amarus. J Ethnopharmacol. 2006; 107: 449-55.

Amarowicz R. Tannins: The new natural antioxidants. Eur J Lipid Sci Tech. 2007; 109: 549-51.

Bakht J, Azra, Shafi M. Antimicrobial activity of Nicotiana tabacum using different solvents extracts. Pak J Bot. 2012; 44: 459-63.

Brownlee M. The pathobiology of diabetic complications: A unifying mechanism. Diabetes 2005; 54; 1615-25.

Deshpande MC, Venkateswarlu V, Babu RK, Trivedi RK. Design and evaluation of oral bioadhesive controlled release formulations of miglitol, intended for prolonged inhibition of intestinal alpha-glucosidases and enhancement of plasma glycogen like peptide-1 levels. Int J Pharm. 2009; 380: 16-24.

Di carli MF, Janisse J, Grunberger G, Ager J. Role of chronic hyperglycemia in the pathogenesis of coronary microvascular dysfunction in diabetes. J Am Coll Cardiol. 2003; 41: 1387-93.

Ezeja MI, Omeh YS. Anti-nociceptive activities of the metha-nolic leaf extract of Nicotiana tabacum (Linn). Continental J Pharmacol Toxicol Res. 2010; 3: 5-10.

Gin H, Rigalleau V, Caubet O, Masquelier J, Aubertin J. Effects of red wine, tannic acid, or ethanol on glucose tolerance in non-insulin dependent diabetic patients and on starch digestibility in vitro. Metabolism. 1999; 48: 1179-83.

Hirsh AJ, Yao SY, Young JD, Cheeseman CI. Inhibition of glucose absorption in the rat jejunum: A novel action of alpha-D-glucosidase inhibitors. Gastroenterol. 1997; 113: 205-11.

Horii S, Fukasse K, Matsuo T, Kameda K, Asano N, Matsui Y. Synthesis and α-D-glucosidase inhibitory activity of N-substituted valiolamine derivatives as potent oral antidiabetic agents. J Med Chem. 1987; 29: 1038-46.

Jacobi J, Jag JJ, Sundram U, Dayoub H, Farjado LF, Cooke JP. Nicotine accelerates angiogenesis and wound healing in genetically diabetic mice. Am J Pathol. 2002; 161: 97-104.

Kazeem MI, Abimbola SG, Ashafa AOT. Inhibitory potential of Gossypium arboreum Linn leaf extracts on diabetes key enzymes (α-amylase and α-glucosidase). Bangladesh J Pharmacol. 2013a; 8: 149-55.

Kazeem MI, Dansu TV, Adeola SA. Inhibitory effect of Azadirachta Indica A. Juss Leaf Extract on the activities of α-amylase and α-glucosidase. Pak J Biological Sci. 2013b; 16: 1358-62.

Kazeem MI, Oyedapo BF, Raimi OG, Adu OB. Evaluation of Ficus exasperata Vahl leaf extracts in the management of Diabetes mellitus in vitro. J Medical Sci. 2013c; 13: 269-75.

Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI. Inhibitory effects of pine bark extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition 2005; 21: 756-61.

Kwon YI, Apostolidis E, Shetty K. In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension. Bioresource technol. 2008a; 99: 2981-88.

Kwon YI, Apostolidis E, Shetty K. Inhibitory potential of wine and tea against ?-amylase and ?-glucosidase for manage-ment of hyperglycemia linked to type 2 diabetes. J Food Biochem. 2008b; 32: 15-31.

Kwon YI, Vattem DA, Shetty K. Evaluation of clonal herbs of Lamiaceae species for management of diabetes and hypertension. Asia Pac J Clin Nutr. 2005; 15: 107-18.

Liu X, Kim J, Li Y, Li J, Liu F, Chen X. Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-Li cells. J Nutr. 2005; 135: 165-71.

Mayur B, Sandesh S, Shruti S, Sung-Yum S. Antioxidant and ?-glucosidase inhibitory properties of Carpesium abrotanoides L. J Medicinal Plant Res. 2010; 4: 1547-53.

Mccue PP, Shetty K. Inhibitory effects of rosmarinic acid extracts on porcine pancreatic mylase in vitro. Asia Pac J Clin Nutr. 2004; 13: 101-106.

Ono K, Hasegawa K, Naiki H, Yamada M. Anti-amyloidogenic activity of tannic acid and its activity to destabilize Alzheimers α-amyloid fibrils in vitro. Biochem Biophys Acta. 2004; 1690: 193-202.

Ren N, Timko M. AFLP analysis of genetic polymorphism and evolutionary relationship among cultivated and wild Nicotiana species. Genome 2001; 44: 559-71.

Ru Q, Wang L, Li W, Wang J, Ding Y. In vitro antioxidant properties of flavonoids and polysaccharides extract from tobacco (Nicotiana tabacum L.) leaves. molecules. 2012; 17: 11281-91.

Shai LJ, Masoko P, Mokgotho MP, Magano SR, Mogale AM, Boaduo N, Eloff JN. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. South Afr J Bot. 2010; 76: 465-70.

Smith C, Marks AD, Lieberman M. Marks basic medical biochemistry: A clinical approach. 2nd ed. USA, Lipincott Williams and Wilkins, Maryland, 2005, pp 2120-36.

Sofowara A. Medical plants and traditional medicine in Africa. Rep. edition, Ibadan, Spectrum Books Ltd., 2006, pp 191-289.

Song W, Wang H, Bucheli P, Zhang P, Wei D, Lu Y. Phytochemical profiles of different Mulberry (Morus. sp.) species from China. J Agric Food Chem. 2009; 57: 9133-40.

Sudha P, Zinjarde SS, Bhargava SY, Kumar AR. Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Compl Alternative Med. 2011; 11: 1-10.

Trease GE, Evans WC. Pharmacognosy. 4th ed. USA, W.B. Saunders, 1996, pp 243-83.

Tundis R, Loizzo MR, Menichini F. Natural products as α-amylase and α-glucosidase inhibitors and their hypoglycemic potential in the treatment of diabetes: An update. Minirev Med Chem. 2010; 10: 31531.



How to Cite

Kazeem, M. I., S. M. Ogungbe, G. M. Saibu, and O. M. Aboyade. “In Vitro Study on the Hypoglycemic Potential of Nicotiana Tabacum Leaf Extracts”. Bangladesh Journal of Pharmacology, vol. 9, no. 2, Mar. 2014, pp. 140-5, doi:10.3329/bjp.v9i2.17540.



Research Articles