In vitro study on the hypoglycemic potential of Nicotiana tabacum leaf extracts

Authors

  • Mutiu Idowu Kazeem Department of Biochemistry, Lagos State University, P. M. B 0001, Ojo, Lagos
  • Semawon Mauton Ogungbe Department of Biochemistry, Lagos State University, PMB 0001, Ojo, Lagos
  • Gbemisola Morounke Saibu Department of Biochemistry, Lagos State University, PMB 0001, Ojo, Lagos
  • Oluwaseyi Mayode Aboyade South African Herbal Science and Medicine Institute, University of the Western Cape, Bellville 7535, South Africa

DOI:

https://doi.org/10.3329/bjp.v9i2.17540

Keywords:

Diabetes, Enzyme inhibition, Hyperglycemia, Nicotiana tabacum, Phytochemical

Abstract

Inhibition of some carbohydrate metabolizing enzymes is one of the modes of action of antihyperglycemic agents. The aim of this study was to investigate the in vitro inhibition of α-amylase and α-glucosidase by extracts of N. tabacum leaf. Powdered leaves were extracted with acetone, ethanol and water, and tested for their ability to inhibit α-amylase from Aspergilus oryzae and α-glucosidase from Saccharomyces cerevisae. The results revealed that aqueous extract of the plant was most effective inhibitor of α-amylase (IC50 5.70 mg/mL) while acetone extract exhibited the best inhibitory potential on α-glucosidase (IC50 4.50 mg/mL). Kinetic studies showed that the mode of inhibition of α-amylase by aqueous extract was non-competitive while that of the acetone extract on α-glucosidase was competitive. The observed inhibitions of α-amylase and α-glucosidase suggest that the leaf extracts of N. tabacum may be useful in the management of Diabetes mellitus, which may due to the presence of phytochemicals.

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Author Biography

Mutiu Idowu Kazeem, Department of Biochemistry, Lagos State University, P. M. B 0001, Ojo, Lagos

Lecturer

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Published

2014-03-29

How to Cite

Kazeem, M. I., S. M. Ogungbe, G. M. Saibu, and O. M. Aboyade. “In Vitro Study on the Hypoglycemic Potential of Nicotiana Tabacum Leaf Extracts”. Bangladesh Journal of Pharmacology, vol. 9, no. 2, Mar. 2014, pp. 140-5, doi:10.3329/bjp.v9i2.17540.

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Section

Research Articles