A double-blind, randomize, placebo-control trial to evaluate the effect of Nigella sativa on palmar arsenical keratosis patients
Keywords:Arsenicosis, Nigella sativa, Palmar keratosis
This study was done to examine the role of Nigella sativa in 28 patients of palmar arsenical keratosis. Patients were randomized into two groups: Group A (n-15) receive capsules of placebo and vitamin E (200 mg) whereas Group B (n= 13) receive capsules of N. sativa oil (500 mg) and vitamin E (200 mg) orally for 8 weeks. The mean (± SD) clinical scoring of palmer arsenical keratosis in Group A before and after treatment was 99.3 ± 21.5 and 62.3 ± 14.3 respectively (37% reduction). On the other hand, in N. sativa oil treated group, the mean clinical score was 119.1 ± 20.3 before treatment which was reduced to 39.2 ± 8.4 (p<0.0001) after treatment (67% reduction). There were 65% reduction of total arsenic in nail of Group A and 30% in Group B. In conclusion, oral administration of N. sativa oil improves the symptom of palmar arsenical keratosis as a result of reduction of body arsenic load.
Al-Hader A, Aqel A, Hasan Z. Hypoglycemic Effects of the volatile oil of Nigella sativa seeds. Pharm Biol. 1993; 31: 96-100.
Al-Jassir MS. Chemical composition and microflora of black cumin (Nigella sativa L.) seeds growing in Saudi Arabia. Food Chemistry.1992; 45: 239-42.
Al-Jishi S, Abuo Hozaifa B. Effect of Nigella sativa on blood hemostatic function in rats. J Ethnopharmacol. 2003; 85: 7-14.
Argos M, Kalra T, Rathouz PJ, Chen Y, Pierce B, Parvez F, Islam T, Ahmed A, Rakibuz-Zaman M, Hasan R, Sarwar G, Slavkovich V, van Geen A, Graziano J, Ahsan H. Arsenic exposure from drinking water, and all-cause and chronicdisease mortalities in Bangladesh (HEALS): A prospective cohort study. The Lancet. 2010; 376: 252-58.
Bamosa AO, Ali BA, Al-Hawsawi ZA. The effect of thymoquinone on blood lipids in rats. Indian J Physiol Pharmacol. 2002; 46: 195-201.
Bamosa AO, Ali BA, Spwayan SA. Effect of oral ingestion of Nigella sativa seeds in some blood parameters. Saudi Pharm J. 1997; 5: 126-29.
Boskabady M, Mohsenpoor N, Takaloo L. Antiasthmatic effect of Nigella sativa in air ways of asthmatic patients. Phytomed. 2010; 17: 707-13.
Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res. 2000; 14: 323-28.
Chen YC, Lin-Shiau SY, Lin JK. Involvement of reactive oxygen species and caspase 3 activation in arsenite induced apoptosis. J. Cell Physiol. 1998; 177: 324-33.
Chowdhury NJA, Mir Misbahuddin M, Rahman MS. Corn extracts lower tissue arsenic level in rat. Bangladesh Med Res Counc Bull. 2009; 35: 21-25.
Dina AN, Misbahuddin M. Randomized double-blind trial to evaluate the effectiveness of topical administration of propylene glycol in arsenical palmer keratosis. Bangladesh J Pharmacol. 2010; 5: 98-102.
Ebru U, Burak U, Yusuf S, Reyhan B, Arif K, Faruk TH, Emin M, Aydin K, Atilla II, Semsettin S, Kemal E. Cardioprotective effects of Nigella sativa oil on cyclosporine A-induced cardiotoxicity in rats. Basic Clin Pharmacol Toxicol. 2008; 103: 574-80.
El-Said SM, Alamri MBS, El-Barak ABS, Alsogair O. Adsorptive removal of arsenite as (III) and arsenate as (V) heavy metals from waste water using Nigella sativa L. Asian J Sci Res. 2009; 2: 96-104.
Fariduddin AKM, Misbahuddin M, Manun MIR, Nahar N. Alcohol extract and residue of spirulina in the prevention of accumulation of arsenic in rats. Bangladesh J Physiol Pharmacol. 2001; 17: 15-17.
Flora SJ, Bhadauria S, Pant SC, Dhaked RK. Arsenic induced blood and brain oxidative stress and its response to some thiol chelators in rats. Life Sci. 2005; 77: 2324-37.
Flora, SJ. Arsenic induced oxidative stress and its reversibility following combined administration of N-acetyl cysteine and meso 2,3-dimercaptosuccinic acid in rats. Clin. Exp. Pharmacol. Physiol. 1999; 26: 865-69.
Gad AM, El-Dakhakhany M, Hassan MM. Studies on the chemical constitution of Egyptian Nigella sativa L oil. Planta Med. 1963; 11: 134-38.
Hall AF. Arsenical keratosis disappearing with vitamin A therapy. Arch Derm Syph. 1946; 53: 154.
Haq A, Abdullatif M, Lobo PI, Khabar KS, Sheth KV, al-Sedairy ST. Nigella sativa: Effect on human lymphocytes and polymorphonuclear leukocyte phagocytic activity. Immunopharmacol. 1995; 30: 147-55.
Hawsawi ZA, Ali BA, Bamosa AO. Effect of Nigella sativa (black seeds) and thymoquinone on blood glucose in albino rats. Ann Saudi Med. 2001; 21: 224-24.
Hosseinzadeh H, Parvardeh S. Anticonvulsant effects of thymoquinone, the major constituent of Nigella sativa seeds, in mice. Phytomedicine. 2004; 11: 56-64.
Hosseinzadeh H, Parvardeh S, Asl M, Sadeghnia H, Ziaee T. Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cerebral ischemia-reperfusion injury in rat hippocampus. Phytomedicine. 2007; 14: 621-27.
Houghton PJ, Zarka R, de las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med. 1995; 61: 33-36.
Islam AZMM, Misbahuddin M, Sikdar S, Biswas A K, Islam Z, Hadiuzzaman, Khandker S, Mahmud IA, Ahmad SA. Randomized controlled trial to evaluate the effectiveness of topical use of salicylic acid for treatment of keratosis in arsenicosis patients. In: Applied research on arsenic in Bangladesh. Misbahuddin M (ed.). Dhaka. World Health Organization (Bangladesh), Director General of Health Services, Govt. of Bangladesh, 2007, pp 92-100.
Kanter M. Effects of Nigella sativa seed extract on ameliorating lung tissue damage in rats after experimental pulmonary aspirations. Acta Histochemica. 2009; 111: 393-403.
Khan MAR, Misbahuddin MS, Khandker S, Ifthaker-Al-Mahmud. Arsenic estimation in foodstuffs of arsenic exposed areas in Bangladesh. In: Applied research on arsenic in Bangladesh. Misbahuddin M (ed.), World Health Organization, Dhaka, 2007, pp 31-42.
Kitchin, KT. Recent advances in arsenic carcinogenesis: Mode of action, animal model system and methylated arsenic metabolites. Toxicol Appl Pharmacol. 2001; 172: 249-61.
Le PM, Benhaddou AA, Settaf A, Cherrah Y, Haddad PS. The petroleum ether extract of Nigella sativa exerts lipid lowering action in the rats. J Ethanopharmacol. 2004; 94: 251-59.
Meddah B, Ducroc R, El Abbes Faouzi M. Nigella sativa inhibits intestinal glucose absorption and improves glucose tolerance in rats. J Ethnopharmacol. 2009; 121: 419-24.
Misbahuddin M, Bashar T, Hossain MA. Effectiveness of garlic oil in the treatment of arsenical palmer keratosis. Bangladesh J Pharmacol. 2013; 8: 22-27.
Misbahuddin M, Islam AZ, Khandker S, Ifthaker-Al-Mahmud, Islam N, Anjumanara. Efficacy of spirulina extract plus zinc in patients of chronic arsenicosis poisoning: a randomized placebo-controlled study. Clin Toxicol (Phila). 2006; 44: 135-41.
Misbahuddin M, Khandker S, Jakariya M. Arsenic contamina-tion of drinking water and foodstuffs. In: Drinking water contaminants in Bangladesh: Focuses on arsenic, fluoride, pesticides, manganese and cyanobacteria. Misbahuddin M, Khandker S (eds). Lambert Academic Publishing, Germany, 2011, pp 12-57.
Najmi A, Nasiruddin M, Khan RA, Haque SF. Effect of Nigella sativa oil on various clinical and biochemical parameters of insulin resistance syndrome. Int J Diabetes Dev Ctries. 2008; 28: 11-14.
Parhizkar S, Latiff LA, Rahman SA, Dollah MA. Preventive effect of Nigella sativa on metabolic syndrome in menopause induced rats. J Med Plants Res. 2011; 5: 1478-84.
Salem M. Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol. 2005; 5: 1749-70.
Umar BU. Effect of hexane extract of spinach in the removal of arsenic from rat. Bangladesh J Pharmacol. 2007; 2: 27-34.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).