Attenuation of hyperglycemia and hyperlipidemia in high calorie fed/streptozotocin-treated rats by hydromethanolic extract of Padina tetrastromatica

Authors

  • Divya S. Mohan Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695 581, Kerala
  • Mini Saraswathy Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695 581, Kerala
  • Muraleedhara Kurup Gopala Kurup Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695 581, Kerala

DOI:

https://doi.org/10.3329/bjp.v9i1.17153

Keywords:

Antihyperglycemic, Antihyperlipidemic, Diabetes mellitus, Padina tetrastromatica

Abstract

In the present study, the effect of defatted hydromethanolic extract of Padina tetrastromatica on carbohydrate metabolism and serum lipid profile were evaluated. Diabetes mellitus was induced in male Wistar rats by feeding high calorie/energy diet for two months followed by a single intraperitoneal injecttion of streptozotocin. Diabetic rats were administered with the extract intragastrically at doses of 150, 300, 450, and 600 mg/kg body weight daily for 45 days. Treatment with graded doses showed a dose dependent reduction in blood glucose and glycated hemoglobin levels. Treatment significantly increased the activity of hexokinase, glucose-6-phosphate dehydrogenase and glycogen content while there was significant reduction in the activity of glucose-6-phosphatase and fructose-1,6-bisphosphatase. Serum lipid profile was also brought back to near normal levels in a dose dependent manner. The present study clearly indicates the antihyperglycemic and hypolipidemic effects of P. tetrastromatica at an optimum dose of 450 mg/kg body weight.

Downloads

Download data is not yet available.
Abstract
2768
Download
1916 Read
219

References

Agardh CD, Bjorgell P, Nlson, EP, The effect of tolbutamide on lipoproteins and lipoprotein lipase and hormone sensitive lipase. Diabetes Res Clin Pract. 1999; 46: 99-108.

Ahmed OM, Abdel-Hamid H, Bastawy M, Hasona NA Antihyperglycemic effects of Plantago ispaghula seeds aqueous extracts in diabetic and hypercholesterolemic rats. J Egypt Ger Soc Zool. 2006; 51: 371-93.

Ahmed OM. The hypoglycemic effect of curcumin and esculetin and their probable mechanism of action in STZ-induced diabetic albino rats. J Egypt Ger Soc Zool. 2005; 46: 351-75.

Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem. 1974; 20: 470-75.

Biddinger SB, Kahn CR. From mice to men: Insights into the insulin resistance syndromes. Annu Rev Physiol. 2006; 68: 123-58.

Bopanna KN, Kannan J, Sushma G, Balaraman R, Rathod SP. Antidiabetic and antihyperglycemic effects of neem seed kernel powder on alloxan diabetic rabbits. Indian J Pharmacol. 1997; 29: 162-67.

Brandstrup N, Kirk JE, Bruni C. Determination of hexokinase in tissues. J Gerontol. 1957; 12: 166-71.

Bucolo G, David H. Quantitative determination of serum triglyceride by use of enzymes. Clin Chem. 1973; 19: 475-82.

Carroll NV, Longley RW, Roe JH. The determination of glycogen in liver and muscle by use of anthrone reagent. J Biol Chem. 1956; 220: 583-93.

Cline GW, Petersen KF, Krssak M, Shen J, Hundal RS, Trajanoski Z, Inzucchi S, Dresner A, Rothman DL, Shulman GI. Impaired glucose transport as a cause of decreased insulin-stimulated muscle glycogen synthesis in type 2 diabetes. N Engl J Med. 1999. 341; 240-46.

Crane RK, Sols A. The association of hexokinase with particulate fractions of brain and other tissue homogenates. J Biol Chem. 1953; 203: 273-92.

Elsner M, Guldbakke B, Tiedge M, Munday R, Lenzen S. Relative importance of transport and alkylation for pancreatic beta-cell toxicity of streptozotocin. Diabetologia 2000; 43: 1528-33.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18: 499-502.

Grover JK, Vats V, Rathi SS. Antihyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol. 2000; 73: 461-70.

Halliwell B, Gutteridge JMC. Antioxidant defences, free radicals in biology and medicine. 3rd ed. Oxford Science, 1999, pp 105-59.

Huang S, Czech MP. The GLUT4 glucose transporter. Cell Metab. 2007; 5: 237-52.

Huang X, Vaag A, Hanson M, Weng J, Laurila E, Goop L. Impaired insulin stimulated expression of the glycogen synthase gene in skeletal muscle of type II diabetic patients in acquired rather than inherited. Clin Endocrin Metabol. 2006; 85: 1584-90.

Kim KY, Nama KA, Kurihara H, Kim SM. Potent alpha-glucosidase inhibitors purified from the red alga Grateloupia elliptica. Phytochemistry 2008; 69: 2820-25.

Kim MS, Kim JY, Choi WH, Lee SS. Effects of seaweed supplementation on blood glucose concentration, lipid profile, and antioxidant enzyme activities in patients with type 2 diabetes mellitus. Nutr Res Pract Summer. 2008; 2: 62-67.

Kornberg A, Horecker BL. Glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase. Methods Enzymology. Colowick SP, Kaplan NO (eds). Vol 1, New York, Academic Press, 1955, pp 323-27.

Lalhlenmawia H, Kumarappani CT, Bhattacharjec BB, Mondal S. Antidiabetic activity of Mallotus roxburghianus leaves in diabetic rats induced by STZ. Pharmacology 2007; 3 : 244-54.

Mayes PA. The pentose phosphate pathway and other pathway of hexose metabolism. In: Harpers biochemistry. Murray RK, Granner DK, Mayes VW (eds.), USA, McGraw-Hill, 2000, pp 219-37.

Mithievre G, Vidal G, Zitovn C, Miriasian C. Glucose-6-phosphatase mRNA and activity are increased to the same extent in liver and kidney of diabetic rats. Diabetes. 1996; 45: 891-96.

Mohsin S, Muraleedhara Kurup G, Arun AR. Anti-inflammatory and antioxidant effect of sulphated polysa-ccharide isolated from marine algae Padina tetrastromatica from Kerala coast. J Pharm Res. 2011; 4: 784-88.

Mohsin S, Muraleedhara Kurup G, Mahadevan R. Effect of Ascophyllan from brown algae Padina tetrastromatica on inflammation and oxidative stress in carrageenan induced rats. Inflammation 2013; (Accepted for publication).

Mohsin S, Muraleedhara Kurup G. Mechanism underlying the anti-inflammatory effect of sulphated polysaccharide from Padina tetrastromatica against carrageenan induced paw edema in rats. Biomed Prev Nutr. 2011; 1: 294-301.

Mu J, Woods J, Zhou YP, Roy RS, Li Z, Zycband E, Feng Y, Zhu L, Li C, Howard AD, Moller DE, Thornberry NA, Zhang BB. Chronic inhibition of dipeptidyl peptidase-4 with a sitagliptin analog preserves pancreatic beta-cell mass and function in a rodent model of type 2 diabetes. Diabetes. 2006; 55: 1695-1704.

Nahla S El-Shenawy, Ismail M Abdel-Nabi. Hypoglycemic effect of Cleome droserifolia ethanolic leaf extract in experimental diabetes and on non-enzymatic antioxidant, glycogen, thyroid hormones and insulin levels. Diabetol Croat. 2006; 35-36.

Nisizawa K, Noda H, Kikuchi R, Watamaba T. The main seaweed foods in Japan. Hydrobiol J. 1987; 151/152: 5-29.

Pari L, Satheesh MA. Effect of pterostilbene on hepatic key enzymes of glucose metabolism in streptozotocin and nicotinamide-induced diabetic rats. Life Sci. 2006; 79: 641-45.

Pontremoli S. Fructose-1,6-diphosphatase I: Rabbit liver (crystalline). Methods Enzymology. Wood AW (ed). Vol 9, New York, Academic Press, 1966, pp 625-31.

Rajkumar M, Uttam KD, Debidas G. Attenuation of hyperglycemia and hyperlipidemia in streptozotocin-induced diabetic rats by aqueous extract of seed of Tamarindus indica. Biol Pharm Bull. 2005; 28: 1172-76.

Rawi SM. Physiological and biochemical evaluation of certain plants extracts as antidiabetic agents. Egypt J Zool. 2000; 32: 112-17.

Robert C, Nordlie, William JA. Methods Enzymology. Wood WA (ed). Vol 9. New York, London. Academic Press, 1966, pp 619-25.

Saltiel AR, Kahn CR. Insulin signalling and the regulation of glucose and lipid metabolism. Nature 2001; 414: 799-806.

Sunil C, Duraipandiyan V, Agastian P, Ignacimuthu S. Antidiabetic effect of plumbagin isolated from Plumbago zeylanica L. root and its effect on GLUT4 translocation in streptozotocin-induced diabetic rats. Food Chem Toxicol. 2012;. 50: 4356-63.

Triplitt CL. New technologies and therapies in the management of diabetes. Am J Manag Care. 2007; 13: S47-S54.

Trivelli LA, Ranney HM, Lai HT. Hemoglobin components in patients with diabetes mellitus. N Engl J Med. 1971; 284: 353-57.

Ugochukwu NH, Babady NE. Antihyperglycaemic effect of aqueous and ethanolic extract of Gongronema latifolium leaves on glucose and glycogen metabolism in liver of normal and streptozotocin-induced diabetic rats. Life Sci. 2003; 73: 1925-38.

Wang HJ, Jin YX, Shen W, Neng J, Wu T, Li YJ, Fu ZW. Low dose streptozotocin (STZ) combined with high energy intake can effectively induce type 2 diabetes through altering the related gene expression. Asia Pac J Clin Nutr. 2007; 16 (Suppl 1): 412-17.

Zhang F, Ye C, Li G, Ding W, Zhou W, Chen G, Luo T, Guang M, Liu Y, Zhang D, Zheng S Yang J, Gu Y, Xie X, Luo M. The rat model of type 2 diabetes mellitus and its glycometabolism characters. Exp Anim. 2003; 52: 401-07.

Additional Files

Published

2014-01-25

How to Cite

Mohan, D. S., M. Saraswathy, and M. K. G. Kurup. “Attenuation of Hyperglycemia and Hyperlipidemia in High Calorie fed/Streptozotocin-Treated Rats by Hydromethanolic Extract of Padina Tetrastromatica”. Bangladesh Journal of Pharmacology, vol. 9, no. 1, Jan. 2014, pp. 37-42, doi:10.3329/bjp.v9i1.17153.

Issue

Section

Research Articles