Hepatoprotective potential of Azima tetracantha and Tribulus terrestris on ferrous sulfate-induced toxicity in rat

  • Manikandaselvi Sambasivam Postgraduate and Research Department of Biochemistry, S. T. E. T. Women’s College, Mannargudi, Tamilnadu
  • R. Ravikumar Department of Chemistry, School of Chemical and Bio-Technology, SASTRA University, Thanjavur, Tamilnadu
  • R. Thinagarbabu Department of Chemistry, School of Chemical and Bio-Technology, SASTRA University, Thanjavur, Tamilnadu
  • C. Davidraj CARISM, SASTRA University, Thanjavur, Tamilnadu
  • S. Arvind Postgraduate and Research Department of Biochemistry, S. T. E. T. Women’s College, Mannargudi, Tamilnadu
Keywords: Azima tetracantha, Ferrous sulphate, Hepatotoxicity, Tribulus terrestris


The present study is to evaluate the antihepatotoxic effect of hydroalcoholic extract of leaf powder of Azima tetracantha and the fruit powder of Tribulus terrestris. Ferrous sulfate was used to induce hepatotoxicity and Silymarin was used as a standard drug. The level of biochemical parameters such as protein, albumin, globulin, HDL, vitamin E, superoxide dismutase and catalase were observed to be decreased and the level of glucose, LDL, VLDL, bilirubin, cholesterol, triglycerides, alkaline phosphatase and TBARS were increased in hepatotoxicity-induced rats. Retrieval of liver parameters to normal level was obtained after the oral administration of herbal drugs. Histopathological studies revealed diminished hepatocellular injury in the herbal drugs treated rats. As a conclusion hydro alcoholic extract of leaf powder of A. tetracantha and fruit powder of T. terrestris were possesses significant hepatoprotective activity.


Download data is not yet available.
379 Read

Author Biography

Manikandaselvi Sambasivam, Postgraduate and Research Department of Biochemistry, S. T. E. T. Women’s College, Mannargudi, Tamilnadu
Assistant Professor


Ahmad A, Najmi AK, Ahmad S, Pal SN, Balani DK. Evaluation of hepatoprotective potential of Jigrine post-treatment against thioacetamide induced hepatic damage. J Ethnopharmacol. 2002; 9: 35-41.

Akhtar MS, Asjad HMM, Bashir S, Malik A, Khalid R, Gulzar F, Irshad N. Evaluation of antioxidant and hepatoprotective effects of Khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (KGA). Bangladesh J Pharmacol. 2013; 8: 44-48.

Balamurugan C, Muthusamy P, Dhonde SM. Observation of the hepatoprotective effect and antioxidant activities of Trianthema decandra Linn. (Ballai sharunnai) roots on carbon tetra chloride treated rats. Bangladesh J Pharmacol. 2008; 32: 83-89.

Bancroft J. Theory and practice of histological technique. 1977, p 411.

Bennett RN, Mellon FA, Roaa EA, Perkins L, Kroon PA. Profiling glucosinolates, flavonoids, alkaloids and other secondary metabolites in tissues of Azima tetracantha Lam. J Agr Food Chem. 2004; 52: 5856-62.

Bhattacharya A, Ramanathan M, Ghosals, Bhattacharya SK. Effect of withania somnifera glycowithanolides on iron-induced hepatatoxicity in rats. Phytother Res. 2000; 14: 568-70.

Boerth J, Strong KM. The clinical utility of milk thistle (Silybum marianum) in cirrhosis of the liver. J Herbal Pharmacotherapy 2002; 2: 11-17.

Doumas BT, Watson WA, Biggs HG. Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chim Acta. 1971; 258: 21-30.

Firdous SM, Sravanthi K, Debnath R, Neeraja K. Protective effect of ethanolic extract and its ethyl acetate and n-butanol fractions of Sechium edule fruits against carbon tetrachloride induced hepatic injury in rats. Int J Pharm Pharm Sci. 2012 ;4: 354-59.

Fraga CG, Leibovitz BE, Toppel AL. Lipid peroxidation measured as TBARS in tissue. Free Radic Med. 1988; 4: 155-61.

Fridwald WT, Levy RT, Fredricleson DS., Estimation of lipoproteins in plasma without use of preparative centrifuge. Clin Chem. 1972; 23: 499.

Hemalatha K, Geetha M, Senthamorai R. The hepatoprotective activity of lawsoia alba on carbon tetrachloride treated rats. Geobios. 2005; 2: 79-82.

Jasulanth A, Begum VH, Akilandeswari S, Bengu M, Manimaran S, Ruckmani K. Effects of Azima tetracantha on dermal wound healing in rats. Hamdard Medicus. 2001; 44: 13-16.

Kakkar R, Das B, Vishwanathan PN. Modified spectrophotometric method of super oxide dismutase. Indian J Biochem Bio. 1984; 21: 130-32.

Kaplan MM. Serum alkaline phospatase-another piece is added to the puzzle. Hepatology.1986; 6: 526-28.

Khan I, Singh V, Chaudhary AK. Hepatoprotective activity of Pinus roxburghii Sarg. wood oil against carbon tetrachloride and ethanol induced hepatotoxicity. Bangladesh J Pharmacol 2012; 7: 94-99.

Kirsch KR, Frith L, Black E, Hoffenberg R. Regulation of albumin synthesis and catabolism by alteration of dietary protein. Nature 1968; 217: 578-79.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem. 1951; 193: 265-75.

Malloy E, Evelyn K. The determination of bilirubin with the photoelectric calorimeter. J Biol Chem. 1937; 119: 481-85.

Pullareddy A, Lokesh BR. Effect of curcumin and eugenol on iron-induced hepatic toxicities in rats. Toxicology 1996; 107: 39-45.

Ramanathan A, Kittusamy R. Antihepatotoxic effect of isolated chitin from Rhizopus oryzae against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2011; 6: 64-67.

Ratnasooriya WD, Jayakody JRA, Premakumara GAS, Ediviweera ERH. Antioxidant activity of water extract of scoparia dulcis. Fitoterapia. 2005; 76: 220-22.

Reilly PM, Schilter HJ, Bulkey GB., Pharmacological approach to tissue injury mediated by free radical and other reactive oxygen metabolites. Am J Surg. 1991; 61: 488-503.

Rice EW. Standard methods in clinical chemistry. Vol.VI. NewYork, Academic Press, 1970; pp. 215-22.

Sinha KA. Colorimetric method of catalase. 1972; 47: 389-94.

Tiez NW. Study group on alkaline phosphatase: A reference method of measurement of alkaline phosphatase activity in human serum. Clin Chem. 1983; 29: 751.

Tuncer MA, Yaymaci B, Sati L, Cayli S, Acar G, Altug T, Demir R. Influence of Tribulus terrestris Linn. extract on lipid profile and endothelial structure in developing atherosclerotic lesions in the aorta of rabbits on a high-cholesterol diet. Acta Histochem. 2009; 142-143.

Winckes J, Tietz NW. Clinical guide to laboratory tests. 1971, p. 246.

Zaspal BJ, Determination of tocopherol in tissue and plasma. Anal Biochem. 1983; 130: 146-50.

Zlatis, Zak, Boyle JA. New method for the direct determination of serum cholesterol. J Lab Clin Med. 1953; 41: 486.

How to Cite
Sambasivam, M., R. Ravikumar, R. Thinagarbabu, C. Davidraj, and S. Arvind. “Hepatoprotective Potential of Azima Tetracantha and Tribulus Terrestris on Ferrous Sulfate-Induced Toxicity in Rat”. Bangladesh Journal of Pharmacology, Vol. 8, no. 3, Sept. 2013, pp. 357-60, doi:10.3329/bjp.v8i3.15579.
Research Articles