Hepatoprotective and nephroprotective activity of hydroalcoholic extract of Ipomoea staphylina leaves

  • Anupam Kanti Bag Department of Pharmacology, Ratnam Institute of Pharmacy, Pidathapolur Village, Muthukur SPSR Nellore, Andhra Pradesh
  • S. M. Firdous Mumtaz Department of Pharmacology, Calcutta Institute of Pharmaceutical Technology and AHS, Uluberia, Howrah 711 316, West Bengal
Keywords: Carbon tetrachloride, Gentamicin, Ipomoea staphylina, Nephroprotective, Nephrotoxicity


The present study was undertaken to investigate the protective effect of hydroalcoholic extract of leaves of Ipomoea staphylina against carbon tertrachloride- and gentamicin-induce hepatotoxicity and nephrotoxicity in rats respectively. The extract (200 mg/kg, p.o) showed hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity  by significantly (p<0.001) reducing the levels of AST, ALT, ALP, total bilirubin and significantly (p<0.001) increasing the serum total protein. The extract also showed nephro-protective activity against gentamicin-induced nephrotoxicity by significantly (p<0.001) reducing the levels of blood urea, blood urea nitrogen (BUN), serum creatinine and significantly (p<0.001) increasing the serum total protein. The extract also improves the histology of the liver and kidney. The total phenolic content and the total flavonoid content of the extract were 94.59 ± 2.54 mg gallic acid equivalent/g and 44.68 ± 2.00 mg rutin equivalent/g respectively. 


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AL-Howiriny TA, AL-Sohaibani MO, AL-Said MS, Rafatullah S. Hepatoprotective properties of Commiphora opobalsamum ("Balessan"), a traditional medicinal plant of Saudi Arabia. Drugs Exp Clin. 2004; 30: 213-20.

Firdous M, Nitesh SC. Evaluation of antiulcer activity of Ipomoea staphylina. Pharmacologyonline 2009; 3: 195-99.

Firdous SM, Raju K. In vivo and in vitro antiinflammatory activity of leaves of Ipomoea staphylina. Int J Pharm Pharm Sci. 2012; 4: 339-43.

Firdous SM, Raju K, Pallab H. Evaluation of hepatoprotective activity of saponin of Momordica dioica roxb. Against CCl4 induced hepatic injury in rats. Pharmacologyonline. 2008; 3: 487-94.

Harlalka GV, Patil CR, Patil MR. Protective effect of Kalanchoe pinnata pers. (crassulaceae) on gentamicin-induced nephrotoxicity in rats. Indian J Pharmacol. 2007; 39: 201-05.

Kaplowitz N, Aw TY, Simon FR, Stolz A. Drug induced hepatotoxicity. Ann Intern Med. 1986; 104: 826.

Kim DO, Jeong SW, Lee CY: Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 2003; 81: 321-26.

Krishna BKL, Mruthunjaya K, Patel JA. Antioxidant and hepatoprotective activity of leaf extract of Justica gendrerussa. Int J Biol Chem. 2009; 3: 99-110.

Levey AS, Atkins R, Coresh J, Cohen EP, Collins AJ, Eckardt KU, Nahas ME, Jaber BL, Jadoul M, Levin A, Powe N R, Rossert J, Wheeler DC, Lameire N, Eknoyan G. Chronic kidney disease as a global public health problem: Approaches and initiatives - a position statement from Kidney Disease Improving Global Outcomes. Kidney Int. 2007; 10: 1-5.

Manokaran S, Jaswanth A, Sengottuvelu S, Nandhakumar J. Hepatoprotective activity of Aerva lanata Linn. against Paracetamol induced hepatotoxicity in rats. Res J Pharm Tech. 2008; 1: 398-400.

OECD, 2002. Acute oral toxicity. Acute oral toxic class method guideline 423 adopted 23.03.1996. In: Eleventh Addendum to the, OECD, guidelines for the testing of chemicals organisation for economics co-operation, development, Paris, June, 2000, p 11.

Park YS, Jung ST, Kang SG, Heo BK, Arancibia-Avila P, Toledo F, Drzewiecki J, Namiesnik J, Gorinstein S. Antioxidants and proteins in ethylene-treated kiwifruits. Food Chem. 2008; 107: 640-48.

Poormoosavi SM, Behmanesh MA, Najafzadeh H. Effect of cimetidine on gentamicin-losartan induced-nephrotoxicity in rats. Afr J Pharm Pharmacol. 2010; 4: 341-45.

Punitha ISR, Shirwaikar A, Shirwaikar A. Antidiabetic activity of benzyltetraisoquinoline alkaloid berberine in streptozotocin nicotinamide induced type 2 diabetic rats. Diabetologia Croatica. 2005; 34: 117-28.

Saleem TSM, Chetty SM, Ramkanth S, Rajan VST, Kumar KM, Gauthaman K. Hepatoprotective herbs: A review. Int J Res Pharm Sci. 2010; 1: 1-5.

Sanjay J, Vinod KD, Neelzesh M, Vijay A. Antioxidant and hepatopraotective activity of hydroalcoholic and aqueous extracts of Amorphophallus campanulatus roxb. tubers. Acta Poloniac Pharm Drug Res. 2009; 66: 423-28.

Rajib A, Monirul I, Israt JB. Hepatoprotective activity of methanol extract of some medicinal plants against carbon tetrachloride-induced hepatotoxicity in rats. Eur J Sci Res. 2009; 37: 302-10.

Reddy DP, Kota R, Renuka S, Anarthe SJ, Nulgumnall, Manjunathaiah R. Isolation, characterization of phytoconstituents and pharmacological screening of Ipomoea staphylina. Asian J Pharm Clin Res. 2013; 6: 30-33.

Wahid AM, Vijay RS, Satish BB. Hepatoprotective activity of hydroalcoholic extract leaves of Alocassie indica. Indian J Exp Biol. 2009; 47: 816-21.

How to Cite
Bag, A., and S. M. Mumtaz. “Hepatoprotective and Nephroprotective Activity of Hydroalcoholic Extract of Ipomoea Staphylina Leaves”. Bangladesh Journal of Pharmacology, Vol. 8, no. 3, May 2013, pp. 263-8, doi:10.3329/bjp.v8i3.14845.
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