Antiproliferative and apoptotic effects of pinocembrin in human prostate cancer cells

  • Zhenyu Chen Dental Hospital, Jilin University, Changchun
  • Azhar Rasul The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024, China; Dental Hospital, Jilin University, Changchun 130041,
  • Chaoyue Zhao Dental Hospital, Jilin University, Changchun
  • Faya Martin Millimouno The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Ichiro Tsuji Department of Public Health, Tohoku University, Sendai 980-8576
  • Takaki Yamamura Food and Nutrition, Morioka College, Iwate,
  • Rehana Iqbal Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan 60800,
  • Mahadev Malhi The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024
  • Xiaomeng Li The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024
  • Jiang Li Dental Hospital, Jilin University, Changchun 130041
Keywords: Apoptosis, LNCaP cell, Pinocembrin, Prostate cancer

Abstract

Pinocembrin, (5, 7-dihydroxyflavanone), has been shown to possess anticancer activity against various cancer cells. However, its effect against prostate cancer cells remained enigmatic. In this study, for the first time, we investigated whether pinocembrin could inhibit growth of human prostate cancer cells. MTT assay and flow cytometric analysis were performed to examine the effects of pinocembrin on cell proliferation, cell cycle, and apoptosis. The results revealed that pinocembrin attenuated the cell viability of both androgen-sensitive (LNCaP) as well as androgen-independent (PC3 and DU-145) prostate cancer cell lines, with different p53 status. Further characterization showed that pinocembrin markedly induced apoptosis of LNCaP cells and arrested cell cycle at S and G2/M phase and involved in the dissipation of mitochondrial membrane potential before culminating in apoptosis in pinocembrin-treated LNCaP cells. These in vitro results suggested that pinocembrin should be further examined for in vivo activity in human prostate cancer.

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Author Biography

Azhar Rasul, The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun 130024, China; Dental Hospital, Jilin University, Changchun 130041,

Postdoctoral Research Fellow

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Published
2013-05-20
How to Cite
Chen, Z., A. Rasul, C. Zhao, F. Millimouno, I. Tsuji, T. Yamamura, R. Iqbal, M. Malhi, X. Li, and J. Li. “Antiproliferative and Apoptotic Effects of Pinocembrin in Human Prostate Cancer Cells”. Bangladesh Journal of Pharmacology, Vol. 8, no. 3, May 2013, pp. 255-62, doi:10.3329/bjp.v8i3.14795.
Section
Research Articles