Pharmacological basis for the medicinal use of Morus alba in gut and airways disorders

  • Munasib Khan Natural Products Research Unit, Department of Biological and Biomedical, Sciences, The Aga Khan University Medical College, Karachi 74800
  • Najeeb-ur -Rehman Natural Products Research Unit, Department of Biological and Biomedical, Sciences, The Aga Khan University Medical College, Karachi-74800
  • Arif-ullah Khan Department of Pharmacy, Kohat University of Science and Technology, Kohat 26000
  • Anwar Hassan Gilani Natural Products Research Unit, Department of Biological and Biomedical, Sciences, The Aga Khan University Medical College, Karachi-74800
Keywords: Airways disorder, Gut, Morus alba

Abstract

Crude extract of Morus alba at 100 mg/kg exhibited protective effect against castor oil-induced diarrhea in mice. In isolated rabbit jejunum, M. alba (0.3-10 mg/mL) inhibited the spontaneous contractions and caused glibenclamide-sensitive inhibition of low K+ (20 mM)-induced contractions, with mild effect on high K+ (80 mM). Similarly, cromakalim caused inhibition of low K+, but not of high K+, while verapamil did not differentiate in its inhibitory effect on two concentrations of K+. M. alba (3.0-30 mg/kg) caused suppression of carbachol (100 µg/kg)-induced increase in inspiratory pressure of anaesthetized rats. In guinea-pig trachea, M. alba completely inhibited low K+ contractions, with partial effect on high K+. M. alba (0.3-1.0 mg/mL) caused leftward shift of isoprenaline-induced inhibitory concentration response curves, like papaverine. These results indicate that M. alba possesses a combination of KATP channel opening, weak Ca++-antagonist and phosphodiesterase inhibitory mechanisms, which explain its medicinal use in hyperactive gut and airways disorders.

Downloads

Download data is not yet available.
Abstract
2228
Download
749 Read
262

References

Abdel-HaqH, Cometa MF, Palmery M, Leone MG, Silvestrini B, Saso L. Relaxant effect of Hydrastis canadensis and its major alkaloids on guinea-pig isolated trachea. Pharmacol Toxicol. 2000; 87: 218-22.

Aguilar-Bryan L, Clement JP, Gonzalez G, Kunjilwar K, Babenko A, Bryan J. Towards understanding the assembly and structure of KATP channels. Physiol Rev. 1998; 78: 227-45.

Baquar SR. Medicinal and Poisonous Plants of Pakistan. Printas, Karachi, 1989, p 291.

Barnes PJ. Drugs for asthma. Br J Pharmacol. 2006; 147: S297-S303.

Baurin N, Arnoult E, Scior T, Do QT, Bernard P. Preliminary screening of some tropical plants for anti-tyrosinase activity. J Ethnopharmacol. 2002; 82: 155-58.

Bown D. Encyclopaedia of herbs and their uses. Dorling Kindersley, London, 1995.

Brown TJ, Raeburn D. RP 49356 and cromakalim relax airway smooth muscle in-vitro by opening a sulphonylurea-sensitive K+ channel: A comparison with nifedipine. J Pharmacol Exp Ther. 1991; 256: 480-85.

Buchheit KH, Fozard JR. KATP channel openers for the treatment of airways hyperactivity. Pulm Pharmacol Ther. 1999; 12: 103-05.

Chen F, Nakashima N, Kimura I, Kimura M. Hypoglycemic activity and mechanisms of extracts from mulberry leaves and Cortex moriradicis in streptozotocin-induced diabetic mice. Yakugaku.Zasshi. 1995; 115: 476-82.

Chiej R. Encyclopaedia of medicinal plants. MacDonald, 1984.

Choi EM, Hwang JK. Effects of Morusalba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264.7 macrophages. Fitoterapia 2005; 76: 608-13.

Cook NS. The pharmacology of potassium channels and their therapeutic potential. Trends Pharmacol Sci. 1988; 9: 21-28.

Croci T, Landi M, Elmonds-Alt X, Le Fur G, Maffrand JP, Manara L. Role of tachykinins in castor oil-induced diarrhoea in rats. Bri J Pharmacol. 1997; 121: 375-80.

Davies MP, McCurrie JR, Wood D. Comparative effects of K+ channel modulating agents on contractions of rat intestinal smooth muscle. Eur J Pharmacol. 1996; 297: 249-56.

Deitmer P, Golenhofen K, Noack T. Comparison of the relaxing effects of cicletanine and cromakalim on vascular smooth muscle. J Cardiovasc Pharmacol. 1992; 20: 35-42.

Deshpande RS, Smitha B, Naveen H, Neelakanta NT. Cultivation of medicinal crops and aromatic crops as a means of diversification in agriculture (consolidated report) 2008, p 205.

DiCarlo G, Autore G, Izzo AA, Maiolino P, Mascolo N, Viola P, Diurno MV, Capasso F. Inhibition of intestinal motility and secretion by flavonoids in mice and rats: Structure-activity relationships. J Pharm Pharmacol. 1993; 45: 1054-59.

Doi K, Kojima T, Makino M, Kimura Y, Fujimoto Y. Studies on the constituents of the leaves of Morus alba L. Chem Pharm Bull. 2001; 49: 151-53.

Duke JA, Ayensu ES. Medicinal plants of China. Inc, 1985.

Edeoga HO, OkwuDE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005; 4: 685-88.

Empfield JR, Russell K, Trainor DA. Potassium channel openers: Therapeutic possibilities. Pharmaceut News. 1995; 6: 23-27.

Fleckenstein A. Specific pharmacology of Ca++ in myocardium, cardiac pacemakers and vascular smooth muscle. Rev Pharmacol Toxicol. 1977; 17: 149-66.

Frank H, Puschmann A, Schusdziarra V, Allescher HD. Functional evidence for a glibenclamide-sensitive K+ channel in rat ileal smooth muscle. Eur J Pharmacol.1994; 271: 379-86.

Gayton AC, Hall JE. Textbook of medical physiology. 9th ed. WB Saunders, Philadelphia, 1996, pp 95-103.

Ghayur MN, Gilani AH. Pharmacological basis for the medicinal use of ginger in gastrointestinaldisorders. Dig Dis Sci. 2005; 50: 1889-97.

Ghayur MN, Khan H, Gilani AH. Antispasmodic, bronchodilator and vasodilator activites of (+)-catechin, a naturally occurring flavonoid. Arch Pharmacol Res. 2007; 30: 970-75.

Gilani AH, Khan A, Jabeen Q, Subhan F, Ghafar R. Antispasmodic and blood pressure lowering effects of Valeriana wallichii are mediated through K+ channel activation. J Ethnopharmacol. 2005; 100: 347-52.

Gilani AH, Shah AJ, Zubair A, Khalid S, Kiani J, Ahmed A, Rasheed M, Ahmad VU. Chemical composition and mechanisms underlying the spasmolytic and bronchodilatory properties of the essential oil of Nepeta cataria L. J Ethnopharmacol. 2009; 121: 405-11.

Gopalakrishnan M, Buckner SA, Shieh CC, Fey T, Fabiyi A, Whiteaker KL, Taber RD, Milicic I, Daza AV, Scott VES, Castle NA, Printzenhoff D, London B, Turner SC, Carroll WA, Sullivan JP, Coghlan MJ, Brune ME. In-vitro and in-vivo characterization of a novel naphthylamide ATP-sensitive K+ channel opener, A-151892. Br J Pharmacol. 2004; 143: 81-90.

Hamilton TC, Weir SW, Weston TH. Comparison of theeffects of BRL34915 and verapamil on electrical and mechanical activity in rat portal vein. Br J Pharmacol.1986; 88: 103-11.

Hong CH, Hur SK, Oh OJ, Kim SS, Nam KA, Lee SK. Evaluation of natural products on inhibition of inducible cyclooxygenase (COX-2) and nitricoxide synthase (iNOS) in cultured mouse macrophage cells. J Ethnopharmacol. 2002; 3: 153-59.

Iwao I, Terada Y. On the mechanism of diarrhea due to castor oil. Jap J Pharmacol. 1962; 12: 137-45.

Jacoby HI, Tomko KA, Steffler C, Lee DKH. Vasorelaxant effect of the potassium cannel activator, RWJ 29009 is tissue selective. Life Sci.1993; 52: 233-38.

Jenkinson DH. Potassium channels-multiplicity and challenges. Br J Pharmacol. 2006; 147: S63-S71.

Khan A, Gilani AH. Selective bronchodilatory effect of Rooibos Tea (Aspalathuslinearis) and its flavonoid: chrysoeriol. Eur J Nutr. 2006; 45: 463-69.

Khan A, Reh

man N, AlKharfy KM, Gilani AH. Antidiarrheal and antispasmodic activities of Salvia officinalis are mediated through activation of K+ channels. Bangladesh J Pharmacol. 2011a; 6: 111-16.

Khan A, Jabeen Q, Gilani AH. Pharmacological basis for the medicinal use of cardamom in asthma. Bangladesh J Pharmacol. 2011b; 6: 34-37.

Khan M, Khan A, Rehman N, Gilani AH. Pharmacological explanation for the use of Juniperus excels in hyperactive gastrointestinal and respiratory disorders. J Nat Med. 2012a; 66: 292-301.

Khan M, Khan A, Rehman N, Gilani AH. Gut and airways relaxant effects of Carum roxburghianum.J Ethnopharmacol. 2012b; 141: 938-46.

Kim SY, Gao JJ, Kang HK. Two flavonoids from the leaves of Morus alba induce differentiation of the human promyelocytic leukemia (HL-60) cell line. Biol Pharm Bull. 2000; 23: 451-55.

Kishii KI, Morimoto T, Nakajima N, Yamazaki K, Tsujitani M, Takayanagi I. Effects of LP-805, a novel vasorelaxant agent, a potassium channel opener on rat thoracic aorta. Gen Pharmacol. 1992; 23: 347-53.

Lee SH, Choi SY, Kim H, Hwang JS, Lee BG, Gao JJ, Kim SY. Mulberroside F isolated from the leaves of Morus alba inhibits melanin biosynthesis. Biol Pharm Bull. 2002; 25: 1045-48.

Lorenz KL, Wells JN. Potentiation of the effects of sodium nitroprusside and isoproterenol by selective phosphodiesterase inhibitors. Mol Pharmacol. 1983; 23: 424-30.

Moura RSD, Mello RFD, Daguinaga S. Inhibitory effect of cromakalim in human detrusor muscle is mediated by glibenclamide-sensitive potassium channels. J Urol. 1993; 149: 1174-77.

Nadkarni KM. Indian Materia Medica. Bombay, Popular Prakashan, 1976, p 816.

National Research Council. Guide for the care and use of laboratory animals. Washington, National Academy Press, 1996, pp 1-7.

Oh H, Ko EK, Jun JY, Oh MH, Park SU, Kang KH, Lee HS, KimYC. Hepatoprotective and free radical scavenging activities of prenylflavonoids, coumarin, and stilbene from Morus alba. Planta Med. 2002; 68: 932-34.

Pelaia G, Gallell L, Vatrella A, Grembiale RD, Maselli R, DeSarro GB, Marsico SA. Potential role of potassium channel openers in the treatment of asthma and chronic obstructive pulmonary disease. Life Sci. 2002; 70: 977-90.

Pietta P. Flavonoids in medicinal plants. In: Flavonoids in health and disease. Rive-Evans CV, Packer L (eds). New York, Marcel Dekker, 1998, pp 61-110.

Poggioli RA,Benelli R,Arletti E,Cavazzuti A. Bertolini: K+ channel openers delay intestinal transit and have antidiarrheal activity. Eur J Pharmacol. 1995; 287: 207-09.

Quest U. Potassium channel openers: Pharmacological and clinical aspects. Fund Clin Pharmacol. 1992; 6: 279-93.

Rang HP, Dale MM, Ritter JM. Pharmacology, 4th edn. New York, Churchill Livingstone, 1999, pp 289-90.

Rehman NU, Khan A, Alkharfy KM, Gilani AH. Pharmacological basis for the medicinal use of Lepidium sativum in airways disorders. Evid-Based Complement Alter Med. 2012; 8 pages.

Reynolds IJ, Gould RJ, Snyder SH. Loperamide: Blockade of calcium channels as a mechanism for antidiarrhoeal effects. J Pharmacol Exp Ther. 1984; 231: 628-32.

Rudy B. Diversity and ubiquity of K+ channels. Neuroscience 1988; 25: 729-49.

Shah AJ, Gilani AH. Bronchodilatory effect of Acorus calamus is mediated through multiple pathways. J Ethnopharmacol. 2010; 131: 471-77.

Shah AJ, Zaidi MA, Sajjad H, Hamidullah, Gilani AH. Antidiarrheal and antispasmodic activities of Vincetoxicum stocksii are mediated through calcium channel blockade. Bangladesh J Pharmacol. 2011a; 6: 46-50.

Shah AJ, Begum S, Hassan SI, Ali SN, Siddiqui BS, Gilani AH. Pharmacological basis for the medicinal use of Psidium guajava leave in hyperactive gut disorders. Bangladesh J Pharmacol. 2011b; 6: 100-06.

Sheng LX, Jian XY. Potassium channels in airway smooth muscle and airway hyperactivity in asthma. Chin Med J. 2005; 118: 574-80.

Shieh CC, Coghlan M, Sullivan JP, Gopalakrishnan M. Potassium channels: molecular defects, diseases and therapeutic opportunities. Pharmacol Rev. 2000; 52: 557-93.

Shinwari ZK, Khan AA, Nakaika T. Medicinal and other useful plants of District Swat Pakistan. Peshawar, Press, Al-Aziz Communications, 2003, p 78.

Usmanghani K, Saeed A, Alam MT. Indusyunic medicine. Karachi, University of Karachi Press, 1997, pp 295-96.

Weinberger M, Hendeles L. Theophylline in asthma. New Eng J Med. 1996; 334: 1380-88.

Weston AH, Edwards G. Recent progress in potassium channel opener pharmacology. Biochem Pharmacol. 1992; 43: 47-54.

Wiart C. Medicinal plants of Southeast Asia. 2nd ed, Malaysia, Pearson, 2002, p 40.

Williamson EM, Okpako DT, Evans FJ. Selection, preparation and pharmacological evaluation of plant material. John Wiley & Sons, Chichester, 1998, pp 15-23.

Published
2012-12-10
How to Cite
Khan, M., N.- ur -Rehman, A.- ullah Khan, and A. Gilani. “Pharmacological Basis for the Medicinal Use of Morus Alba in Gut and Airways Disorders”. Bangladesh Journal of Pharmacology, Vol. 7, no. 4, Dec. 2012, pp. 289-98, doi:10.3329/bjp.v7i4.12873.
Section
Research Articles