Effects of neferine on TGF-β1 induced proliferation and gremlin expression in hepatic stellate cells

  • Xiaofei Li Department of Infectious Diseases, Yiwu Central Hospital, Zhejiang 322000
  • Lianqing Lou Department of Infectious Diseases, Yiwu Central Hospital, Zhejiang
  • Shuang Wu Department of Infectious Diseases, Yiwu Central Hospital, Zhejiang
  • Yongxin Chen Department of Infectious Diseases, Yiwu Central Hospital, Zhejiang
  • Lingzhen Jin Department of Infectious Diseases, Yiwu Central Hospital, Zhejiang
Keywords: Gremlin, Hepatic fibrosis, Neferine, Transforming growth factor-β1

Abstract

To clarify the possible molecular mechanism underlying the anti-fibrotic effect of neferine (Nef), we investigated the in vitro effects of Nef on the proliferation of hepatic stellate cells and expressions of gremlin, TGF-β1 and α-SMA in these cells. TGF-β1 had no influence on the proliferation of HSC-T6 cells (p>0.05) but significantly up-regulated the mRNA and protein expressions of gremlin, TGF-β1 and α- SMA in these cells (p<0.005). Nef could inhibit the proliferation of HSC-T6 cells in a dose and time dependent manner, and down-regulate the mRNA and protein expression of gremlin, TGF-β1 and α- SMA in these cells (p<0.005). Nef is an effective drug that can inhibit the proliferation of hepatic stellate cells to improve the hepatic fibrosis. Gremlin involves in the occurrence and development of hepatic fibrosis and may become a promising target in the treatment of hepatic fibrosis.

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Published
2012-08-23
How to Cite
Li, X., L. Lou, S. Wu, Y. Chen, and L. Jin. “Effects of Neferine on TGF-β1 Induced Proliferation and Gremlin Expression in Hepatic Stellate Cells”. Bangladesh Journal of Pharmacology, Vol. 7, no. 3, Aug. 2012, pp. 169-72, doi:10.3329/bjp.v7i3.11298.
Section
Research Articles