Effects of neferine on TGF-β1 induced proliferation and gremlin expression in hepatic stellate cells
To clarify the possible molecular mechanism underlying the anti-fibrotic effect of neferine (Nef), we investigated the in vitro effects of Nef on the proliferation of hepatic stellate cells and expressions of gremlin, TGF-β1 and α-SMA in these cells. TGF-β1 had no influence on the proliferation of HSC-T6 cells (p>0.05) but significantly up-regulated the mRNA and protein expressions of gremlin, TGF-β1 and α- SMA in these cells (p<0.005). Nef could inhibit the proliferation of HSC-T6 cells in a dose and time dependent manner, and down-regulate the mRNA and protein expression of gremlin, TGF-β1 and α- SMA in these cells (p<0.005). Nef is an effective drug that can inhibit the proliferation of hepatic stellate cells to improve the hepatic fibrosis. Gremlin involves in the occurrence and development of hepatic fibrosis and may become a promising target in the treatment of hepatic fibrosis.
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Copyright (c) 2012 Xiaofei Li, Lianqing Lou, Shuang Wu, Yongxin Chen, Lingzhen Jin
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