Effects of crude flavonoids from tatary buckwheat on alloxan-induced oxidative stress in mice
The present study was undertaken to evaluate the effects of crude flavonoids from tatary buckwheat (FTB) on alloxan-induced oxidative stress in mice. After induction of diabetes, diabetic mice were randomly divided into four groups: one diabetic control group and three different doses of FTB (100, 200 and 400 mg/kg) treated groups, with non-diabetic mice used as the normal control group. The mice were intragastrically administered once daily for 28 days. Fasting blood glucose (FBG), serum insulin, superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and malondialdehyde (MDA) were measured. The results showed that FTB could significantly reduce FBG levels and MDA contents, and increase serum insulin levels, SOD, GPx and CAT activities. These data suggested that FTB possess hypoglycemic effects and could reduce alloxan-induced oxidative stress in mice. Therefore, FTB might be of use as an antidiabetic drug.
Aronson D. Hyperglycemia and the pathobiology of diabetic complications. Adv Cardiol. 2008; 45: 1-16.
Bîcu M, Moţa M, Panduru NM, Grăunţeanu C, Moţa E. Oxidative stress in diabetic kidney disease. Rom J Intern Med. 2010; 48: 307-12.
Bikopoulos G, da Silva Pimenta A, Lee SC, Lakey JR, Der SD, Chan CB, Ceddia RB, Wheeler MB, Rozakis-Adcock M. Ex vivo transcriptional profiling of human pancreatic islets following chronic exposure to monounsaturated fatty acids. J Endocrinol. 2008; 196: 455-64.
Chen XM, Jin J, Tang J, Wang ZF, Wang JJ, Jin LQ, Lu JX. Extraction, purification, characterization and hypoglycemic activity of a polysaccharide isolated from the root of Ophiopogon japonicus. Carbohyd Polym. 2011; 83: 749-75.
Drel VR, Sybirna N. Protective effects of polyphenolics in red wine on diabetes associated oxidative/nitrative stress in streptozotocin-diabetic rats. Cell Biol Int. 2010; 34: 1147-53.
Egan JM, Asplin CM, Drumheller MA, Kerrigan JR, Scott J, Martha PM Jr, Evans WS. Glucose-stimulated insulin release by individual pancreatic beta-cells: Potentiation by glyburide. Proc Soc Exp Biol Med. 1991; 196: 203-09.
Fabjan N, Rode J, Kosir IJ, Wang Z, Zhang Z, Kreft I. Tartary buckwheat (Fagopyrum tataricum Gaertn.) as a source of dietary rutin and quercitrin. J Agric Food Chem. 2003; 51: 6452-55.
Gong F, Li F, Zhang L, Li J, Zhang Z, Wang G. Hypoglycemic effects of crude polysaccharide from purslane. Int J Mol Sci. 2009; 10: 880-88.
Grover JK, Vats V, Rathi SS. Anti-hyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol. 2000; 73: 461-70.
Guo XD, Ma YJ, Parry J, Gao JM, Yu LL, Wang M. Phenolics content and anti-oxidant activity of tartary buckwheat from different locations. Molecules 2011; 16: 9850-67.
Hordern SV, Wright JE, Umpleby AM, Shojaee-Moradie F, Amiss J, Russell-Jones DL. Comparison of the effects on glucose and lipid metabolism of equipotent doses of insulin detemir and NPH insulin with a 16 hours euglycaemic clamp. Diabetologia. 2005; 48: 420-26.
Jia ZS, Tang MC, Wu JM. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64: 555-99.
King GL, Loeken MR. Hyperglycemia-induced oxidative stress in diabetic complications. Histochem Cell Biol. 2004; 122: 333-38.
Li D, Li X, Ding X. Composition and antioxidative properties of the flavonoid-rich fractions from tartary buckwheat grains. Food Sci Biotechnol. 2010; 19: 711-16.
Li F, Li Q, Gao D, Peng Y. The optimal extraction parameters and anti-diabetic activity of flavonoids from Ipomoea batatas leaf. Afr J Tradit Complement Altern Med. 2009; 6: 195-202.
Li F, Tang H, Xiao F, Gong J, Peng Y, Meng X. Protective effect of salidroside from Rhodiolae radix on diabetes-induced oxidative stress in mice. Molecules. 2011; 16: 9912-24.
Li FL, Gong FY, Gong JL, Zhang Z, Xiao FR. Hypoglycemic effects of flavonoids from tartary buckwheat in alloxan-induced diabetic. Shanghai, 6th International Conference on Bioinformatics and Biomedical Engineering, 2012, pp 957-60.
Lopes JP, Oliveira SM, Soares Fortunato J. Oxidative stress and its effects on insulin resistance and pancreatic beta-cells dysfunction: Relationship with type 2 diabetes mellitus complications. Acta Med Port. 2008; 21: 293-302.
Maritim AC, Sanders RA, Watkins JB 3rd. Diabetes, oxidative stress, and anti-oxidants: A review. J Biochem Mol Toxicol. 2003; 17: 24-38.
Niedowicz DM, Daleke DL. The role of oxidative stress in diabetic complications. Cell Biochem Biophys. 2005; 43: 289-330.
Ren W, Qiao Z, Wang H, Zhu L, Zhang L, Lu Y, Cui Y, Zhang Z, Wang Z. Tartary buckwheat flavonoid activates caspase 3 and induces HL-60 cell apoptosis. Methods Find Exp Clin Pharmaco. 2001; 23: 427-32.
Rohilla A, Ali S. Alloxan Induced Diabetes: Mechanisms and Effects. Int J Res Pharma Biomedical Sci. 2012; 3: 819-21.
Sivakumar S, Palsamy P, Subramanian SP. Attenuation of oxidative stress and alteration of hepatic tissue ultrastructure by D-pinitol in streptozotocin-induced diabetic rats. Free Radic Res. 2010; 44: 668-78.
Spadella CT, Suarez OA, Lucchesi AN, Marques SF, Cataneo AJ. Effects of pancreas transplantation on oxidative stress in pulmonary tissue from alloxan-induced diabetic rats. Transplant Proc. 2010; 42: 2087-91.
Wang M, Liu JR, Gao JM, Parry JW, Wei YM. Anti-oxidant activity of tartary buckwheat bran extract and its effect on the lipid profile of hyperlipidemic rats. J Agric Food Chem. 2009; 57: 5106-12.
Xue SX, Chen XM, Lu JX, Jin LQ. Protective effect of sulfated Achyranthes bidentata polysaccharides on streptozotocin-induced oxidative stress in rats. Carbohy Poly. 2009; 75: 415-19.
Yu ZF, Wang M. Technique of macroporous resin for tartary buckwheat total flavonoids. Zhongguo Zhong Yao Za Zhi. 2007; 32: 585-89.
Zhang HN, He JH, Yuan L, Lin ZB. In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage. Life Sci. 2003; 73: 2307-19.
Zhang WM, Ma JH, Kun YC, Gong FY. Evaluation of the antioxidant activities of total flavonoids from tartary buckwheat. J Med Plants Res. 2012; 6: 1461-67.
Copyright (c) 2012 Fayong Gong, Fenglin Li, Wanming Zhang, Jing Li, Zhong Zhang
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).