Trichoderma harzianum : A new fungal source for the production of cyclosporin A
Pure cultures of Trichoderma harzianum, T. aureoviride, T. reesei, T. koningii and T. hemantum were checked for their potential to produce cyclosporin A. Production medium used for drug production was consisted of: glucose, 5%; peptone, 1%; KH2PO4, 0.5%; KCL, 0.25% (w/v). Whereas, butyl acetate was used to extract the fermentation medium for cyclosporine A. This was then analyzed through High Performance Liquid Chromatography and the chromatograms obtained were compared with that of cyclosporine and with the external standard cyclosporin A 98.5% pure. Only chromatogram of T. harzianum showed a peak at 2.779, which was comparable with both the standards. Mass spectroscopy of this peak showed [CsA + H] + ion of m/z 1203. The amount of drug calculated was 44.06 µg/mL.
Agathos SN, Marshell JW, Maraiti C, Parekh R, Modhosingh C. Physiological and genetic factors for process development of cyclosporin fermentation. J Industrial Microbiol. 1986; 1: 39-48.
Ajith PS, Lakshmidevi N. Effect of volatile and non-volatile compounds from Trichoderma spp. against Colletotrichum capsici incitant of Anthracnose on Bell peppers. Nat Sci. 2010; 8: 265-69.
Borel JF, Feurer C, Magnee AC, Stahelin H. Effects of the new anti-lymphocytic peptide cyclosporin A in animals. Immunology 1977; 32: 1017-25.
Borel JF. Cyclosporin and its future. In: Cyclosporin. Borel JF (ed). Progress in Allergy. Vol. 38. Switzerland, Karger, Basel, 1986, pp 9-18.
Balakrishnan K, Pandey A. Growth and Cyclosporin A production by an indigenously isolated strain of Tolypocladium inflatum. Folia Microbiol. 1996; 41: 401-06.
Deo YM, Gaucher GM. Semi-continuous and continuous production of penicillin G by Penicillium chrysogenum cells immobilized in ?-carrageenan beads. Biotechnol Bioeng. 1984; 2: 285-95.
Dreyfuss M, Harri E, Hofmann H, Kobel H, Pache W. Tscherter H. Cyclosporin A and C: New metabolites from Trichoderma polysporum. European J Appl Microbiol. 1976; 3: 12533.
Gams W. Tolypocladium. Eine Hyphomycetengattung mit geschwollenen Phialiden. Persoonia. 1971; 6: 18591.
IARC. Pharmaceutical Drugs. IARC Monographs on the evaluation of carcinogenic risk of chemicals to humans. International Agency for Research on Cancer, 1990, p 415.
Park NS, Park HJ, Han K, Kim ES. Heterologous expression of novel cytochrome P450 hydroxylasegenes from Sebekia benihana. J Microbiol Biotechnol. 2006; 16: 295-98.
Sallam LA, El-Refai AM, Hamdi AH, El-Minofi A, Abd-Elsalam. Studies on the applications of immobilization technique for the production of cyclosporin A by a local strain of Aspergillus terreus. J Gen Appl Microbial. 2005; 51: 143-49.
Sallam LRA, Abdel-Monem H, El-Refai, Abdel-Hamid A, Hassaan A, El-Minofi, Ibrahim S, Abdel-Salam. Role of some fermentation parameters on cyclosporin A production by a new isolate of A. terreus. J Gen Appl Microbial. 2003; 49: 321-28.
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