Quality assessment of sputum for Acid Fast Bacilli(AFB) test in microbiology laboratories before and after implementation of Standard Operating Procedure (SOP): a comparative study

Authors

  • K Khadeza Department Of Microbiology, Dhaka Medical College, Dhaka
  • AHM Kamal Department Of Anatomy, Dhaka Medical College, Dhaka
  • M Naima Department Of Microbiology, Dhaka Medical College, Dhaka
  • SM Shamsuzzaman Department Of Microbiology, Dhaka Medical College, Dhaka

DOI:

https://doi.org/10.3329/bjms.v11i1.9824

Keywords:

Quality assessment, Sputum for AFB test, Microbiology laboratories, Standard Operating Procedure (SOP).

Abstract

Aims: To evaluate the present status of microbiology laboratory by comparing the test results of investigator with that of laboratory staff for sputum for Acid Fast Bacilli (AFB) withZiehl-NeelsenStaining (Z-N Staining) at primary and secondary level andto assess the present status of a microbiology laboratory for sputum for Acid Fast Bacilli (AFB) withZiehl-NeelsenStaining (Z-N Staining) at tertiary level.

Material and Methods:

Type of study : Cross sectional, descriptive type of study.

Place of study: Department of Microbiology of Dhaka Medical College,Narsingdi Sadar Hospital,Narsingdi,PolashUpzilla Health Complex ,Polash, Narsingdi andDOTS centers.

Duration of study: From July, 2007 to June,2008

Method of sampling: Non probability, purposive and convenient sampling

Sample Size: Sputum for AFB: 300 sputum samples were collected for detection of AFB by direct Z-N staining, Z-N staining after bleach centrifugation and Auramine phenol staining before implementing SOP. After following SOP 150 sputum samples were collected and tested in direct Z-N method.

Results: In the present study, before SOP out of 100 sputum smear at each level, discrepancy was found in 3% cases at primary level, 2% cases at secondary level and 1% case at tertiary level.After following SOP out of 50 sputum smear at each level, discrepancy was reduced to 2% cases at primary level and no discrepancy was found at secondary and tertiary level.

Conclusion: Each laboratory must have SOP for laboratory testing to set the minimum acceptable standard for every test in order to improve and maintain the quality of laboratory services.

DOI: http://dx.doi.org/10.3329/bjms.v11i1.9824

BJMS 2012; 11(1): 51-56

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How to Cite

Khadeza, K., Kamal, A., Naima, M., & Shamsuzzaman, S. (2012). Quality assessment of sputum for Acid Fast Bacilli(AFB) test in microbiology laboratories before and after implementation of Standard Operating Procedure (SOP): a comparative study. Bangladesh Journal of Medical Science, 11(1), 51–56. https://doi.org/10.3329/bjms.v11i1.9824

Issue

Vol. 11 No. 1 (2012)

Section

Original Articles

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