Ameliorative effect of Silk Fibroin against 5-Fluorouracil (5-FU)-induced gastrointestinal damage in rats

Authors

  • Ahmet Özer Şehirli Department of Pharmacology, Faculty of Dentistry, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey
  • Aslı Aykaç Department of Biophysics, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey
  • Hanife Özkayalar Departments of Pathology, Faculty of Medicine, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey
  • Gökçe Savtekin Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey and Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of City Island, 9945 Famagusta, North Cyprus, Mersin 10, Turkey
  • Serkan Sayıner Department of Biochemistry, Faculty of Veterinary Medicine, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey
  • Serkan Sayıner Department of Biochemistry, Faculty of Veterinary Medicine, Near East University, 99138 Nicosia, North Cyprus, Mersin 10, Turkey

DOI:

https://doi.org/10.3329/bjms.v22i4.68679

Keywords:

Silk Fibroin; 5-Fluoruracil; iNOS; CD4 ; CD8

Abstract

Objective: 5-Fluorouracil (5-FU), a widely administered anti-cancer drug, causes gastrointestinal damage through various mechanisms. This study aimed to investigate the ameliorative effect of silk fibroin (SF), which has anti-inflammatory and antioxidant properties, against 5 FU-induced gastrointestinal damage.

Materials and methods: Wistar albino rats were divided into 3 groups; Control group, 5- FU group (30mg/kg/day on days 0, 2, 4), and 5-FU+SF (30 mg/kg/day 5-FU on days 0, 2, 4 + 600 mg/kg/day SF for 14 days). At the end of the experiment, stomach and intestinal tissue samples were collected and immunohistochemically iNOS, caspase-3, CD4+ and CD8+ were measured. Besides, structural damage was assessed with hematoxylin-eosin and caspase-3 and 9 activities were evaluated using western blot analysis.

Results and Discussion: It was determined that iNOS, caspase-3, caspase-9 activities, CD4+ and CD8+ levels and structural damage significantly increased in both stomach and intestinal tissues. Furthermore, elevated markers were regressed almost to control levels in SF administrated group.

Conclusion: SF may have an ameliorative effect, thus reducing the gastrointestinal damage caused by 5 FU. More clinical and scientific research should be conducted as the silk fibroin may be a suitable candidate against the adverse effects of chemotherapeuic agents.

Bangladesh Journal of Medical Science Vol. 22 No. 04 October’23 Page : 907-915

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Published

2023-09-07

How to Cite

Şehirli, A. Özer ., Aykaç, A. ., Özkayalar, H. ., Savtekin, G. ., Sayıner, S. ., & Sayıner, S. . (2023). Ameliorative effect of Silk Fibroin against 5-Fluorouracil (5-FU)-induced gastrointestinal damage in rats. Bangladesh Journal of Medical Science, 22(4), 907–915. https://doi.org/10.3329/bjms.v22i4.68679

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Original Articles