All-Trans Retinoic Acid Reduces Matrix Metalloproteinase-2 and Increases E-Cadherin Levels in BeWo Choriocarcinoma Cells

Authors

  • Tatit Nurseta Department of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University – RSUD Dr. Saiful Anwar, Malang, East Java, Indonesia
  • Stefani Harumsari Resident of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
  • Nanda Agus Prasetya Resident of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia
  • I Wayan Agung Indrawan Department of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University – RSUD Dr. Saiful Anwar, Malang, East Java, Indonesia

DOI:

https://doi.org/10.3329/bjms.v22i2.64992

Keywords:

ATRA, BeWo cell line; choriocarcinoma; MMP-2; E-cadherin

Abstract

Background: Choriocarcinoma is a malignant trophoblastic tumor that can degrade the uterine basement membrane to facilitate local and distant metastasis. Matrix metalloproteinase-2 (MMP-2) is a vital proteinase produced by trophoblasts and can cleave type IV collagen in the basement membrane of the uterine epithelium. In addition, E-cadherin controls cell adhesion, which is associated with tumorprogression, and loss of E-cadherin function is associated with metastasis. Vitamin A analogues, including all-trans retinoic acid (ATRA), exhibit anticancer properties, including metastasis inhibition.This study aimed to investigate the ability of ATRA to prevent trophoblast invasion and metastasis in choriocarcinoma by assessing MMP-2 and E-cadherin activity.

Methods: This study administered various doses of ATRA to the BeWo (American Type Culture Collection CCL-98) choriocarcinoma cell line and assessed MMP-2 and E-cadherin activity through flow cytometry. The research was performed at the Biomedical Laboratory of the Faculty of Medicine of Universitas Brawijaya Malang, East Java, Indonesia.

Results: BeWo cells were exposed to 0, 50, 100, 200, 400, or 800 μg/mL ATRA. MMP-2 was downregulated after ATRA treatment. The greatest reduction in MMP-2 level was observed after 200 μg/mL ATRA treatment, but this result was not statistically significant (P = 0.550). However, ATRA treatment significantly reduced the number of BeWo cells with low E-cadherin levels (P = 0.012).

Conclusion: ATRA downregulates MMP-2 and upregulates E-cadherin. Further in vitro and animal studies are required to evaluate the ability of ATRA to inhibit metastasis.

Bangladesh Journal of Medical Science Vol. 22 No. 02 April’23 Page : 336-340

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Published

2023-04-11

How to Cite

Nurseta, T. ., Harumsari, S. ., Prasetya, N. A. ., & Indrawan, I. W. A. . (2023). All-Trans Retinoic Acid Reduces Matrix Metalloproteinase-2 and Increases E-Cadherin Levels in BeWo Choriocarcinoma Cells. Bangladesh Journal of Medical Science, 22(2), 336–340. https://doi.org/10.3329/bjms.v22i2.64992

Issue

Vol. 22 No. 2 (2023)

Section

Original Articles

License

Copyright (c) 2023 Tatit Nurseta, Stefani Harumsari, Nanda Agus Prasetya, I Wayan Agung Indrawan

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