Tocotrienol-Rich Fraction Modulates Genes Expression in Oxidative Stress-induced Caenorhabditis e legans

  • Goon Jo Aan Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
  • Noralisa Abdul Karim Cell Therapy Centre, Universiti Kebangsaan Malaysia Medical Center, Kuala Lumpur, Malaysia
  • Mohd Shahril Aszrin Zainudin Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
  • Suzana Makpol Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
Keywords: tocotrienol; C.elegans; antioxidant; oxidative stress

Abstract

Objective: The study was done to determine the effect of Tocotrienol rich fraction (TRF) on the expression of RNAs in C. elegans under oxidative stress.

Methods: The nematodes were divided into 4 groups and treated accordingly: control; TRF; hydrogen peroxide (H202); TRF treatment before and after H202-induction (TRF+H202+TRF). Expressions of RNAs were analyzed with Affymetrix Genechip C. elegans Genome Array and Genespring GX11 software where differentially expressed genes were further analyzed using gene ontology (GO). Selected genes (unc-15, cit-1.2, ftn-1, rsks-1, unc-4 and daf-12) were analyzed with RT-qPCR to validate the results.

Results: TRF modulated the expression of 314 genes involved in determination of adult lifespan, regulation of growth and lipid modification. A total of 440 genes involved in RNA metabolic processes, transcription, growth and differentiation of muscle and nerve cells were differently expressed following H202 induction. TRF treatment before and after H202- induction resulted in 438 differentially expressed genes involved in RNA metabolic processes, transcription, response to xenobiotic stimulus and protein amino acid phosphorylation.

Conclusion: TRF modulates the expression of genes involved in the regulation of lifespan in C. elegans.

Bangladesh Journal of Medical Science Vol.18(4) 2019 p.711-721

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Abstract
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Published
2019-08-30
How to Cite
Aan, G., Karim, N., Zainudin, M. S., & Makpol, S. (2019). Tocotrienol-Rich Fraction Modulates Genes Expression in Oxidative Stress-induced Caenorhabditis e legans. Bangladesh Journal of Medical Science, 18(4), 711-721. https://doi.org/10.3329/bjms.v18i4.42874
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Original Articles