Experimental research of Harmine hydrochloride effect on internal organs

  • Galina U Zhanaidarova Karaganda State Medical University, Karaganda, Kazakhstan
  • Roza Zh Yessimova Karaganda State Medical University, Karaganda, Kazakhstan
  • Kulash T Nurseitova Karaganda State Medical University, Karaganda, Kazakhstan
  • Roza B Seidakhmetova Karaganda State Medical University, Karaganda, Kazakhstan
  • Leila I Arystan Karaganda State Medical University, Karaganda, Kazakhstan
  • Sergazy M Adekenov CEO JSC ISPH «Phytohimiya», Karaganda, Kazakhstan
  • Nurlan N Nauryzov Karaganda State Medical University, Karaganda, Kazakhstan
  • Bakhyt Kh Berikbaeva Karaganda State Medical University, Karaganda, Kazakhstan
Keywords: preclinical studies, chronic toxicity, harmine hydrochloride capsules, morphological examination, internal organs.

Abstract

Objective. The purpose of thisstudy was to determine the chronic toxicity of the drug “Harmine hydrochloride, capsules” for preclinical evaluation of its safety.

Materials and methods.The experiment was performed on 48 CD-1 rats. Harmine hydrochloride was injectedto the animals intragastrically at doses of 2.5 mg / kg/per day, 5 mg / kg/per day, 9 mg / kg/per day, 10 mg / kg/per day for 3 months. After 3 months, the animals were withdrew from the experiment, internal organs (brain, heart, spleen, adrenals) were weighed and set in 10% neutral formalin. Histological specimenswere mounted in accordance with standard procedures.

Results and Discussion. All morphological, histopathological changes, in addition to mortality and bodyweight changes were recorded.Microtome cuts, 5 micrometers thick, were stained with hematoxylin and eosin.

Conclusion. As a result of the morphological study, there was no toxic effect of harmine hydrochloride at doses of 2.5 mg / kg, 5 mg / kg, 9 mg / kg and 10 mg / kg on the brain, at doses of 2.5 mg / kg and 5 mg / kg on the structure of the heart, spleen and adrenal glands. At doses of 9 mg / kg and 10 mg / kg, there is an initial toxic dose-dependent effect on the heart, spleen and adrenal glands.

Bangladesh Journal of Medical Science Vol.18(3) 2019 p.598-606

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Abstract
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Published
2019-05-30
How to Cite
Zhanaidarova, G., Yessimova, R., Nurseitova, K., Seidakhmetova, R., Arystan, L., Adekenov, S., Nauryzov, N., & Berikbaeva, B. (2019). Experimental research of Harmine hydrochloride effect on internal organs. Bangladesh Journal of Medical Science, 18(3), 598-606. https://doi.org/10.3329/bjms.v18i3.41635
Section
Original Articles