Centella asiatica decrease bax expression In prefrontal cortex of rat models
Keywords:Centella asiatica; prefrontal cortex; bax; chronic restraint stress
Introduction: Centella asiatica is a herb, which has neuroprotective properties, thus, it can be used to prevent neural damage caused by stress and increased glucocorticoid level. Madecassoside, asiaticoside, asiatic acid, and madecassic acid that are contained inside Centellaasiatica extract have antioxidant and neuroprotective properties.
Method: This research is an experimental research using an animal model with post test-only control group design. We used samples from biologically stored product, in the form of 25 rat-brain tissue paraffin blocks. These brain tissues originated from 25 rat models that were divided into groups P1, P2, P3, P4, and P5. Group P1 was intervened with restrain stress.Group P2, P3, and P4 was intervened with restrain stress and ethanolic extract of Centella asiaticaat 150, 300, and 600 mg/kgBW/days, respectively. Group P5 was intervened with stress and fluoxetinat 10 mg/kgBW/days. On day 23, brain tissues were terminated, perfused, and extracted. Brain tissues were then made into paraffin blocks, turned into preparation pieces, histochemically stained, and cells that expressed caspace-bax were counted.
Results: There was a statistically significant difference of Bax expression between P1 group and P3, P4, and P5 (p=0,01). Bax expression in group P1 was significantly higher than P2, P3, P4, and P5. There was no statistically significant difference of Bax expression between group P1 and P2 (p=0,08).
Conclusion: Ethanolic extract of Centella asiatica was able to decrease Bax expression in prefrontal cortex of chronic restraint stress induced Spraque Dawley rat models.
Bangladesh Journal of Medical Science Vol.18(1) 2019 p.112-117
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