Association between polymorphism of lys198asn endothelin-1 gene and endothelin-1 plasma level in javanese obesity population

  • Dewi Karita Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia
  • Ahmad Hamim Sadewa Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia
  • Pramudji Hastuti Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada
Keywords: endothelin-1; obesity; polymorphism; vasoconstrictor; ET-1.

Abstract

Background: Endothelin-1 plays a role as a vasoconstrictor and also inhibits the expression of adiponectin in obesity. The Lys198Asn polymorphism of the endothelin-1 gene will increase the level of endothelin-1 plasma and increase the risk factor of obesity.

Objective: In this study, we investigated the relationship between Lys198Asn polymorphism of endothelin-1 gene and endothelin-1 plasma level among individuals with obesity in Javanese population.

Method: This study was conducted on 61 obese subjects and 65 control subjects. All subjects were measured for anthropometrics and endothelin-1 plasma level by ELISA (Enzyme-linked Immune Sorbent Assay). The polymorphism of Lys198Asn of ET-1 (Endothelin-1) was screened by using PCRRFLP (Polymerase Chain Reaction- Restriction Fragment Length Polymorphism).

Results: TT genotype was a risk factor of obesity (OR 5.344 CI=95% 1.77-16.16) compared with GG genotype. Levels of endothelin-1 plasma were higher in obese subjects than that of control subjects (p=0.013), with TT genotype having the highest endothelin-1 plasma level (p<0.001).

Conclusion: The Lys198Asn polymorphism of endothelin-1 gene plays a role in increasing endothelin-1 plasma levels and is risk factors of obesity.

Bangladesh Journal of Medical Science Vol.18(1) 2019 p.46-49

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Author Biographies

Dewi Karita, Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia

Department Of Biochemistry

Ahmad Hamim Sadewa, Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada, Indonesia

Department Of Biochemistry

Pramudji Hastuti, Department Of Biochemistry, Medical Faculty Universitas Gadjah Mada

Department Of Biochemistry

Published
2018-12-30
How to Cite
Karita, D., Sadewa, A., & Hastuti, P. (2018). Association between polymorphism of lys198asn endothelin-1 gene and endothelin-1 plasma level in javanese obesity population. Bangladesh Journal of Medical Science, 18(1), 46-49. https://doi.org/10.3329/bjms.v18i1.39546
Section
Original Articles