An in vitro study to elucidate the effects of Septilin TM on immune pathways

Authors

  • Mujeeb Hoosen (Coordinator of Unani-Tibb), School of Natural Medicine, Faculty of Community and Health Sciences, The University of the Western Cape, Bellville
  • Edmund John Pool (Professor of Microbiology), The Department of Medical Biosciences, Faculty of Natural Sciences, The University of the Western Cape, Bellville

DOI:

https://doi.org/10.3329/bjms.v17i2.35877

Keywords:

SeptilinTM, immune pathways biomarkers, immunomodulatory, herbal medicinal products, whole blood cultures

Abstract

Objective: The use of herbal immunomodulatory preparations to prevent and treat immunological complications is increasing in popularity. Rigorous in vitro, in vivo and clinical trial studies are needed to ensure safety, quality and efficacy for the wellbeing of patients. SeptilinTM, a proprietary herbal medicinal product has been reported to have immunomodulatory effects. This study investigated the in vitro effects of SeptilinTM on biomarkers of specific immune pathways by using whole blood culture assays (WBC).

Materials and Methods: Stimulated and unstimulated WBC have been incubated with the product. Enzyme linked immunosorbent assays have been used to screen for IL-6, IL-10, and IFN γ as biomarkers for inflammation, humoral immunity, and cell mediated immunity, respectively.

Results: SeptilinTM had no effect on the release of IL-6 production by (lipopolysaccharide) LPS stimulated WBC across all concentrations tested. However, SeptilinTM induced significant higher levels (P<0.001) of IL-6 release in unstimulated WBC across all concentrations between 0µg/ml-258µg/ml. SeptilinTM had no effect on the release of IL-10 release in unstimulated WBC across all concentrations. However the presence of SeptilinTM in phytohaemagglutinin (PHA) stimulated WBC induced significant higher release (P<0.01) of IL-10 release at concentrations between 64.5µg/ml- 258µg/ml when compared to the control. The presence of SeptilinTM in unstimulated WBC had no effect on the release of IFN γ production across all concentrations. The presence of SeptilinTM in PHA stimulated WBC release of IFN γ is inconclusive.

Conclusion: This study shows that SeptilinTM has immunomodulatory effects on WBC in vitro.

Bangladesh Journal of Medical Science Vol.17(2) 2018 p.238-244

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Author Biography

Mujeeb Hoosen, (Coordinator of Unani-Tibb), School of Natural Medicine, Faculty of Community and Health Sciences, The University of the Western Cape, Bellville



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Published

2018-03-23

How to Cite

Hoosen, M., & Pool, E. J. (2018). An in vitro study to elucidate the effects of Septilin TM on immune pathways. Bangladesh Journal of Medical Science, 17(2), 238–244. https://doi.org/10.3329/bjms.v17i2.35877

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Section

Original Articles