Studies to determine antidiarrhoeal and spasmolytic activities of extract of Aegle marmelos. L fruit

Authors

  • Syed Intasar Husain Taqvi Department of Pharmacology, Federal Urdu University of Arts, Science and Technology, Karachi
  • Atiqur Rahman Pharmacology Section, Pharmaceutical Research Centre, PCSIR Labs; complex, Karachi
  • Mohammad Ali Versiani Dr Iqbal Choudhry Research Lab, Department of Chemistry, Federal Urdu University of Arts, Science and Technology, Karachi
  • Hina Imran Pharmacology Section, Pharmaceutical Research Centre, PCSIR Labs; complex, Karachi
  • Amna Khatoon Dr Iqbal Choudhry Research Lab, Department of Chemistry, Federal Urdu University of Arts, Science and Technology, Karachi
  • Tehmina Sohail Pharmacology Section, Pharmaceutical Research Centre, PCSIR Labs; complex, Karachi

DOI:

https://doi.org/10.3329/bjms.v17i2.35872

Keywords:

antidiarrhoeal, spasmolytic, calcium channel blocking activity, mice ileum

Abstract

Objective: To investigate and determine the antidiarrhoeal and spasmolytic potential of fruit of Aegle marmelos is due to CCBs compounds and not tannic acid.

Materials and methods: The extract of ripe and dry fruit of A. marmelos (Am.Cr) was prepared in methanol: water (70:30). The antidiarrhoeal activities of the extract, loperamide and tannic acid were studied in vivo; in castor oil induced diarrhoeal model in mice whereas spasmolytic effect was studied in vitro; in isolated mice ileum. Calcium channel blocking (CCB) activity was investigated after preincubation of mice ileum by Am. Cr or loperamide and subsequent adding of K+80 mM.

Results: Am. Cr inhibited castor oil-induced diarrhoea: onset of diarrhoea, total no. of faeces, and total no. of wet faeces dose-dependently, the dose of 800 mg/mL was found statistically significant, total weight of feces and total weight of wet faeces were also inhibited. Concentration-dependent inhibition of spontaneous contraction and contractile effect of K+80 mM after pre-incubation by Am. Cr were confirmed in isolated mice ileum. The results of Am. Cr are comparable with Loperamide. Tannic acid produced neither antidiarrhoeal effect nor exhibited CCB activity, however relaxant effect was observed in isolated mice ileum.

Conclusion: The antidiarrhoeal and spasmolytic effects of Am.Cr may be mediated through compounds which posses CCB effect. Tannic acid exhibited relaxant effect only which is not sufficient evidence scientifically to classify as antidiarrhoeal.

Bangladesh Journal of Medical Science Vol.17(2) 2018 p.205-211

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Author Biography

Syed Intasar Husain Taqvi, Department of Pharmacology, Federal Urdu University of Arts, Science and Technology, Karachi



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Published

2018-03-23

How to Cite

Taqvi, S. I. H., Rahman, A., Versiani, M. A., Imran, H., Khatoon, A., & Sohail, T. (2018). Studies to determine antidiarrhoeal and spasmolytic activities of extract of Aegle marmelos. L fruit. Bangladesh Journal of Medical Science, 17(2), 205–211. https://doi.org/10.3329/bjms.v17i2.35872

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Original Articles